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Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake

Snake venoms are composed of a complex mixture of active proteins and peptides which induce a wide range of toxic effects. Envenomation by Bothrops jararaca venom results in hemorrhage, edema, pain, tissue necrosis and hemolysis. In this work, the effect of a mixture of two secodolastane diterpenes...

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Autores principales: Domingos, Thaisa Francielle Souza, Vallim, Magui Aparecida, Cavalcanti, Diana Negrão, Sanchez, Eládio Flores, Teixeira, Valéria Laneuville, Fuly, André Lopes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272259/
https://www.ncbi.nlm.nih.gov/pubmed/25699595
http://dx.doi.org/10.3390/molecules20033515
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author Domingos, Thaisa Francielle Souza
Vallim, Magui Aparecida
Cavalcanti, Diana Negrão
Sanchez, Eládio Flores
Teixeira, Valéria Laneuville
Fuly, André Lopes
author_facet Domingos, Thaisa Francielle Souza
Vallim, Magui Aparecida
Cavalcanti, Diana Negrão
Sanchez, Eládio Flores
Teixeira, Valéria Laneuville
Fuly, André Lopes
author_sort Domingos, Thaisa Francielle Souza
collection PubMed
description Snake venoms are composed of a complex mixture of active proteins and peptides which induce a wide range of toxic effects. Envenomation by Bothrops jararaca venom results in hemorrhage, edema, pain, tissue necrosis and hemolysis. In this work, the effect of a mixture of two secodolastane diterpenes (linearol/isolinearol), previously isolated from the Brazilian marine brown alga, Canistrocarpus cervicornis, was evaluated against some of the toxic effects induced by B. jararaca venom. The mixture of diterpenes was dissolved in dimethylsulfoxide and incubated with venom for 30 min at room temperature, and then several in vivo (hemorrhage, edema and lethality) and in vitro (hemolysis, plasma clotting and proteolysis) assays were performed. The diterpenes inhibited hemolysis, proteolysis and hemorrhage, but failed to inhibit clotting and edema induced by B. jararaca venom. Moreover, diterpenes partially protected mice from lethality caused by B. jararaca venom. The search for natural inhibitors of B. jararaca venom in C. cervicornis algae is a relevant subject, since seaweeds are a rich and powerful source of active molecules which are as yet but poorly explored. Our results suggest that these diterpenes have the potential to be used against Bothropic envenomation accidents or to improve traditional treatments for snake bites.
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spelling pubmed-62722592018-12-31 Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake Domingos, Thaisa Francielle Souza Vallim, Magui Aparecida Cavalcanti, Diana Negrão Sanchez, Eládio Flores Teixeira, Valéria Laneuville Fuly, André Lopes Molecules Article Snake venoms are composed of a complex mixture of active proteins and peptides which induce a wide range of toxic effects. Envenomation by Bothrops jararaca venom results in hemorrhage, edema, pain, tissue necrosis and hemolysis. In this work, the effect of a mixture of two secodolastane diterpenes (linearol/isolinearol), previously isolated from the Brazilian marine brown alga, Canistrocarpus cervicornis, was evaluated against some of the toxic effects induced by B. jararaca venom. The mixture of diterpenes was dissolved in dimethylsulfoxide and incubated with venom for 30 min at room temperature, and then several in vivo (hemorrhage, edema and lethality) and in vitro (hemolysis, plasma clotting and proteolysis) assays were performed. The diterpenes inhibited hemolysis, proteolysis and hemorrhage, but failed to inhibit clotting and edema induced by B. jararaca venom. Moreover, diterpenes partially protected mice from lethality caused by B. jararaca venom. The search for natural inhibitors of B. jararaca venom in C. cervicornis algae is a relevant subject, since seaweeds are a rich and powerful source of active molecules which are as yet but poorly explored. Our results suggest that these diterpenes have the potential to be used against Bothropic envenomation accidents or to improve traditional treatments for snake bites. MDPI 2015-02-18 /pmc/articles/PMC6272259/ /pubmed/25699595 http://dx.doi.org/10.3390/molecules20033515 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Domingos, Thaisa Francielle Souza
Vallim, Magui Aparecida
Cavalcanti, Diana Negrão
Sanchez, Eládio Flores
Teixeira, Valéria Laneuville
Fuly, André Lopes
Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake
title Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake
title_full Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake
title_fullStr Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake
title_full_unstemmed Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake
title_short Effect of Diterpenes Isolated of the Marine Alga Canistrocarpus cervicornis against Some Toxic Effects of the Venom of the Bothrops jararaca Snake
title_sort effect of diterpenes isolated of the marine alga canistrocarpus cervicornis against some toxic effects of the venom of the bothrops jararaca snake
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272259/
https://www.ncbi.nlm.nih.gov/pubmed/25699595
http://dx.doi.org/10.3390/molecules20033515
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