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Urine and Serum Metabolite Profiling of Rats Fed a High-Fat Diet and the Anti-Obesity Effects of Caffeine Consumption

In this study, we investigated the clinical changes induced by a high fat diet (HFD) and caffeine consumption in a rat model. The mean body weight of the HFD with caffeine (HFDC)-fed rat was decreased compared to that of the HFD-fed rat without caffeine. The levels of cholesterol, triglycerides (TGs...

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Autores principales: Kim, Hyang Yeon, Lee, Mee Youn, Park, Hye Min, Park, Yoo Kyoung, Shon, Jong Cheol, Liu, Kwang-Hyeon, Lee, Choong Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272342/
https://www.ncbi.nlm.nih.gov/pubmed/25689639
http://dx.doi.org/10.3390/molecules20023107
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author Kim, Hyang Yeon
Lee, Mee Youn
Park, Hye Min
Park, Yoo Kyoung
Shon, Jong Cheol
Liu, Kwang-Hyeon
Lee, Choong Hwan
author_facet Kim, Hyang Yeon
Lee, Mee Youn
Park, Hye Min
Park, Yoo Kyoung
Shon, Jong Cheol
Liu, Kwang-Hyeon
Lee, Choong Hwan
author_sort Kim, Hyang Yeon
collection PubMed
description In this study, we investigated the clinical changes induced by a high fat diet (HFD) and caffeine consumption in a rat model. The mean body weight of the HFD with caffeine (HFDC)-fed rat was decreased compared to that of the HFD-fed rat without caffeine. The levels of cholesterol, triglycerides (TGs), and free fatty acid, as well as the size of adipose tissue altered by HFD, were improved by caffeine consumption. To investigate the metabolites that affected the change of the clinical factors, the urine and serum of rats fed a normal diet (ND), HFD, and HFDC were analyzed using ultra performance liquid chromatography quadruple time-of-flight mass spectrometry (UPLC-Q-TOF-MS), gas chromatography (GC-TOF-MS), and linear trap quadruple mass spectrometry (LTQ-XL-MS) combined with multivariate analysis. A total of 68 and 52 metabolites were found to be different in urine and serum, respectively. After being fed caffeine, some glucuronide-conjugated compounds, lysoPCs, CEs, DGs, TGs, taurine, and hippuric acid were altered compared to the HFD group. In this study, caffeine might potentially inhibit HFD-induced obesity and we suggest possible biomarker candidates using MS-based metabolite profiling.
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spelling pubmed-62723422018-12-13 Urine and Serum Metabolite Profiling of Rats Fed a High-Fat Diet and the Anti-Obesity Effects of Caffeine Consumption Kim, Hyang Yeon Lee, Mee Youn Park, Hye Min Park, Yoo Kyoung Shon, Jong Cheol Liu, Kwang-Hyeon Lee, Choong Hwan Molecules Article In this study, we investigated the clinical changes induced by a high fat diet (HFD) and caffeine consumption in a rat model. The mean body weight of the HFD with caffeine (HFDC)-fed rat was decreased compared to that of the HFD-fed rat without caffeine. The levels of cholesterol, triglycerides (TGs), and free fatty acid, as well as the size of adipose tissue altered by HFD, were improved by caffeine consumption. To investigate the metabolites that affected the change of the clinical factors, the urine and serum of rats fed a normal diet (ND), HFD, and HFDC were analyzed using ultra performance liquid chromatography quadruple time-of-flight mass spectrometry (UPLC-Q-TOF-MS), gas chromatography (GC-TOF-MS), and linear trap quadruple mass spectrometry (LTQ-XL-MS) combined with multivariate analysis. A total of 68 and 52 metabolites were found to be different in urine and serum, respectively. After being fed caffeine, some glucuronide-conjugated compounds, lysoPCs, CEs, DGs, TGs, taurine, and hippuric acid were altered compared to the HFD group. In this study, caffeine might potentially inhibit HFD-induced obesity and we suggest possible biomarker candidates using MS-based metabolite profiling. MDPI 2015-02-13 /pmc/articles/PMC6272342/ /pubmed/25689639 http://dx.doi.org/10.3390/molecules20023107 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Hyang Yeon
Lee, Mee Youn
Park, Hye Min
Park, Yoo Kyoung
Shon, Jong Cheol
Liu, Kwang-Hyeon
Lee, Choong Hwan
Urine and Serum Metabolite Profiling of Rats Fed a High-Fat Diet and the Anti-Obesity Effects of Caffeine Consumption
title Urine and Serum Metabolite Profiling of Rats Fed a High-Fat Diet and the Anti-Obesity Effects of Caffeine Consumption
title_full Urine and Serum Metabolite Profiling of Rats Fed a High-Fat Diet and the Anti-Obesity Effects of Caffeine Consumption
title_fullStr Urine and Serum Metabolite Profiling of Rats Fed a High-Fat Diet and the Anti-Obesity Effects of Caffeine Consumption
title_full_unstemmed Urine and Serum Metabolite Profiling of Rats Fed a High-Fat Diet and the Anti-Obesity Effects of Caffeine Consumption
title_short Urine and Serum Metabolite Profiling of Rats Fed a High-Fat Diet and the Anti-Obesity Effects of Caffeine Consumption
title_sort urine and serum metabolite profiling of rats fed a high-fat diet and the anti-obesity effects of caffeine consumption
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272342/
https://www.ncbi.nlm.nih.gov/pubmed/25689639
http://dx.doi.org/10.3390/molecules20023107
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