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Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species

The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropic...

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Autores principales: Raveendar, Sebastin, Lee, Gi-An, Jeon, Young-Ah, Lee, Yun Jeong, Lee, Jung-Ro, Cho, Gyu-Taek, Cho, Joon-Hyeong, Park, Jong-Hyun, Ma, Kyung-Ho, Chung, Jong-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272350/
https://www.ncbi.nlm.nih.gov/pubmed/25608853
http://dx.doi.org/10.3390/molecules20011543
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author Raveendar, Sebastin
Lee, Gi-An
Jeon, Young-Ah
Lee, Yun Jeong
Lee, Jung-Ro
Cho, Gyu-Taek
Cho, Joon-Hyeong
Park, Jong-Hyun
Ma, Kyung-Ho
Chung, Jong-Wook
author_facet Raveendar, Sebastin
Lee, Gi-An
Jeon, Young-Ah
Lee, Yun Jeong
Lee, Jung-Ro
Cho, Gyu-Taek
Cho, Joon-Hyeong
Park, Jong-Hyun
Ma, Kyung-Ho
Chung, Jong-Wook
author_sort Raveendar, Sebastin
collection PubMed
description The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch (Vicia sativa subsp. sativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species.
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spelling pubmed-62723502018-12-28 Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species Raveendar, Sebastin Lee, Gi-An Jeon, Young-Ah Lee, Yun Jeong Lee, Jung-Ro Cho, Gyu-Taek Cho, Joon-Hyeong Park, Jong-Hyun Ma, Kyung-Ho Chung, Jong-Wook Molecules Communication The temperate and herbaceous genus Vicia L. is a member of the legume tribe Fabeae of the subfamily Papilionoideae. The genus Vicia comprises 166 annual or perennial species distributed mainly in Europe, Asia, and North America, but also extending to the temperate regions of South America and tropical Africa. The use of simple sequence repeat (SSR) markers for Vicia species has not been investigated as extensively as for other crop species. In this study, we assessed the potential for cross-species amplification of cDNA microsatellite markers developed from common vetch (Vicia sativa subsp. sativa). For cross-species amplification of the SSRs, amplification was carried out with genomic DNA isolated from two to eight accessions of 22 different Vicia species. For individual species or subspecies, the transferability rates ranged from 33% for V. ervilia to 82% for V. sativa subsp. nigra with an average rate of 52.0%. Because the rate of successful SSR marker amplification generally correlates with genetic distance, these SSR markers are potentially useful for analyzing genetic relationships between or within Vicia species. MDPI 2015-01-16 /pmc/articles/PMC6272350/ /pubmed/25608853 http://dx.doi.org/10.3390/molecules20011543 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Raveendar, Sebastin
Lee, Gi-An
Jeon, Young-Ah
Lee, Yun Jeong
Lee, Jung-Ro
Cho, Gyu-Taek
Cho, Joon-Hyeong
Park, Jong-Hyun
Ma, Kyung-Ho
Chung, Jong-Wook
Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species
title Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species
title_full Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species
title_fullStr Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species
title_full_unstemmed Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species
title_short Cross-Amplification of Vicia sativa subsp. sativa Microsatellites across 22 Other Vicia Species
title_sort cross-amplification of vicia sativa subsp. sativa microsatellites across 22 other vicia species
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272350/
https://www.ncbi.nlm.nih.gov/pubmed/25608853
http://dx.doi.org/10.3390/molecules20011543
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