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Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia
A Streptomyces strain MS-6-6 with promising anti-tuberculous activity was isolated from soil samples in Saudi Arabia. The nucleotide sequence of its 16S rRNA gene (1426 bp) evidenced a 100% similarity to Streptomyces mutabilis. Through an anti-tuberculous activity-guided approach, a polyketide macro...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272392/ https://www.ncbi.nlm.nih.gov/pubmed/25648598 http://dx.doi.org/10.3390/molecules20022576 |
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author | Yassien, Mahmoud A. Abdallah, Hossam M. El-Halawany, Ali M. Jiman-Fatani, Asif A. M. |
author_facet | Yassien, Mahmoud A. Abdallah, Hossam M. El-Halawany, Ali M. Jiman-Fatani, Asif A. M. |
author_sort | Yassien, Mahmoud A. |
collection | PubMed |
description | A Streptomyces strain MS-6-6 with promising anti-tuberculous activity was isolated from soil samples in Saudi Arabia. The nucleotide sequence of its 16S rRNA gene (1426 bp) evidenced a 100% similarity to Streptomyces mutabilis. Through an anti-tuberculous activity-guided approach, a polyketide macrolide was isolated and identified as treponemycin (TP). The structure of the isolated compound was determined by comprehensive analyses of its 1D and 2D NMR as well as HRESI-MS. In addition to the promising anti-tuberculous activity (MIC = 13.3 µg/mL), TP showed broad spectrum of activity against the Gram positive, Gram negative strains, and Candida albicans. Improvement of TP productivity (150%) was achieved through modification in liquid starch nitrate medium by replacing KNO(3) with corn steep liquor and yeast extract or tryptone, and removing CaCO(3) and K(2)HPO(4). The follow up of TP percentage as well as its metabolites profile for each media was assessed by LC/DAD/MS. |
format | Online Article Text |
id | pubmed-6272392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62723922018-12-13 Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia Yassien, Mahmoud A. Abdallah, Hossam M. El-Halawany, Ali M. Jiman-Fatani, Asif A. M. Molecules Article A Streptomyces strain MS-6-6 with promising anti-tuberculous activity was isolated from soil samples in Saudi Arabia. The nucleotide sequence of its 16S rRNA gene (1426 bp) evidenced a 100% similarity to Streptomyces mutabilis. Through an anti-tuberculous activity-guided approach, a polyketide macrolide was isolated and identified as treponemycin (TP). The structure of the isolated compound was determined by comprehensive analyses of its 1D and 2D NMR as well as HRESI-MS. In addition to the promising anti-tuberculous activity (MIC = 13.3 µg/mL), TP showed broad spectrum of activity against the Gram positive, Gram negative strains, and Candida albicans. Improvement of TP productivity (150%) was achieved through modification in liquid starch nitrate medium by replacing KNO(3) with corn steep liquor and yeast extract or tryptone, and removing CaCO(3) and K(2)HPO(4). The follow up of TP percentage as well as its metabolites profile for each media was assessed by LC/DAD/MS. MDPI 2015-02-02 /pmc/articles/PMC6272392/ /pubmed/25648598 http://dx.doi.org/10.3390/molecules20022576 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yassien, Mahmoud A. Abdallah, Hossam M. El-Halawany, Ali M. Jiman-Fatani, Asif A. M. Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia |
title | Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia |
title_full | Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia |
title_fullStr | Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia |
title_full_unstemmed | Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia |
title_short | Anti-Tuberculous Activity of Treponemycin Produced by a Streptomyces Strain MS-6-6 Isolated from Saudi Arabia |
title_sort | anti-tuberculous activity of treponemycin produced by a streptomyces strain ms-6-6 isolated from saudi arabia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272392/ https://www.ncbi.nlm.nih.gov/pubmed/25648598 http://dx.doi.org/10.3390/molecules20022576 |
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