Cargando…

Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta

Ligusticum jeholense has been used as the traditional medicine ‘Go-Bon’ (Chinese name, Gao-ben) in China and Korea. Considering the increased use of medicinal herbs to treat hypertension, in this study, we aimed to investigate the mechanisms of the vasorelaxation effect caused by L. jeholense. We te...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Bumjung, Lee, Kyungjin, Chinannai, Khanita Suman, Ham, Inhye, Bu, Youngmin, Kim, Hocheol, Choi, Ho-Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272402/
https://www.ncbi.nlm.nih.gov/pubmed/26065836
http://dx.doi.org/10.3390/molecules200610721
_version_ 1783377148248064000
author Kim, Bumjung
Lee, Kyungjin
Chinannai, Khanita Suman
Ham, Inhye
Bu, Youngmin
Kim, Hocheol
Choi, Ho-Young
author_facet Kim, Bumjung
Lee, Kyungjin
Chinannai, Khanita Suman
Ham, Inhye
Bu, Youngmin
Kim, Hocheol
Choi, Ho-Young
author_sort Kim, Bumjung
collection PubMed
description Ligusticum jeholense has been used as the traditional medicine ‘Go-Bon’ (Chinese name, Gao-ben) in China and Korea. Considering the increased use of medicinal herbs to treat hypertension, in this study, we aimed to investigate the mechanisms of the vasorelaxation effect caused by L. jeholense. We tested the methanol (MeOH) extract of L. jeholense root and rhizoma for vasorelaxant effects; while using an isolated organ-chamber technique, L. jeholense extract (LJE) induced relaxation in the rat aortic rings by stimulating vascular endothelial and smooth muscle cells. LJE showed concentration-dependent relaxant effects on endothelium-intact and endothelium-denuded aortic rings pre-contracted with both phenylephrine (PE) and potassium chloride (KCl) in Krebs-Henseleit (KH) buffer. The vasorelaxant effect of LJE was partly attenuated by pre-treatment with glibenclamide or 4-aminopyridine (4-AP) as K(+) channel blockers. Moreover, LJE showed concentration-dependent inhibition of vasoconstriction by Ca(2+) supplementation in the aortic rings that were pre-contracted with PE or KCl in Ca(2+)-free KH buffer. In addition, a combination of LJE and nifedipine, pre-incubated further, decreased PE-induced contractions. The results suggested that LJE-induced vasorelaxation were related to blocking K(+) channels and inhibiting entry of extracellular Ca(2+) via receptor-operative Ca(2+) channels (ROCCs) or voltage-dependent Ca(2+) channels (VDCCs).
format Online
Article
Text
id pubmed-6272402
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62724022018-12-31 Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta Kim, Bumjung Lee, Kyungjin Chinannai, Khanita Suman Ham, Inhye Bu, Youngmin Kim, Hocheol Choi, Ho-Young Molecules Article Ligusticum jeholense has been used as the traditional medicine ‘Go-Bon’ (Chinese name, Gao-ben) in China and Korea. Considering the increased use of medicinal herbs to treat hypertension, in this study, we aimed to investigate the mechanisms of the vasorelaxation effect caused by L. jeholense. We tested the methanol (MeOH) extract of L. jeholense root and rhizoma for vasorelaxant effects; while using an isolated organ-chamber technique, L. jeholense extract (LJE) induced relaxation in the rat aortic rings by stimulating vascular endothelial and smooth muscle cells. LJE showed concentration-dependent relaxant effects on endothelium-intact and endothelium-denuded aortic rings pre-contracted with both phenylephrine (PE) and potassium chloride (KCl) in Krebs-Henseleit (KH) buffer. The vasorelaxant effect of LJE was partly attenuated by pre-treatment with glibenclamide or 4-aminopyridine (4-AP) as K(+) channel blockers. Moreover, LJE showed concentration-dependent inhibition of vasoconstriction by Ca(2+) supplementation in the aortic rings that were pre-contracted with PE or KCl in Ca(2+)-free KH buffer. In addition, a combination of LJE and nifedipine, pre-incubated further, decreased PE-induced contractions. The results suggested that LJE-induced vasorelaxation were related to blocking K(+) channels and inhibiting entry of extracellular Ca(2+) via receptor-operative Ca(2+) channels (ROCCs) or voltage-dependent Ca(2+) channels (VDCCs). MDPI 2015-06-10 /pmc/articles/PMC6272402/ /pubmed/26065836 http://dx.doi.org/10.3390/molecules200610721 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Bumjung
Lee, Kyungjin
Chinannai, Khanita Suman
Ham, Inhye
Bu, Youngmin
Kim, Hocheol
Choi, Ho-Young
Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta
title Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta
title_full Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta
title_fullStr Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta
title_full_unstemmed Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta
title_short Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta
title_sort endothelium-independent vasorelaxant effect of ligusticum jeholense root and rhizoma on rat thoracic aorta
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272402/
https://www.ncbi.nlm.nih.gov/pubmed/26065836
http://dx.doi.org/10.3390/molecules200610721
work_keys_str_mv AT kimbumjung endotheliumindependentvasorelaxanteffectofligusticumjeholenserootandrhizomaonratthoracicaorta
AT leekyungjin endotheliumindependentvasorelaxanteffectofligusticumjeholenserootandrhizomaonratthoracicaorta
AT chinannaikhanitasuman endotheliumindependentvasorelaxanteffectofligusticumjeholenserootandrhizomaonratthoracicaorta
AT haminhye endotheliumindependentvasorelaxanteffectofligusticumjeholenserootandrhizomaonratthoracicaorta
AT buyoungmin endotheliumindependentvasorelaxanteffectofligusticumjeholenserootandrhizomaonratthoracicaorta
AT kimhocheol endotheliumindependentvasorelaxanteffectofligusticumjeholenserootandrhizomaonratthoracicaorta
AT choihoyoung endotheliumindependentvasorelaxanteffectofligusticumjeholenserootandrhizomaonratthoracicaorta