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Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta
Ligusticum jeholense has been used as the traditional medicine ‘Go-Bon’ (Chinese name, Gao-ben) in China and Korea. Considering the increased use of medicinal herbs to treat hypertension, in this study, we aimed to investigate the mechanisms of the vasorelaxation effect caused by L. jeholense. We te...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272402/ https://www.ncbi.nlm.nih.gov/pubmed/26065836 http://dx.doi.org/10.3390/molecules200610721 |
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author | Kim, Bumjung Lee, Kyungjin Chinannai, Khanita Suman Ham, Inhye Bu, Youngmin Kim, Hocheol Choi, Ho-Young |
author_facet | Kim, Bumjung Lee, Kyungjin Chinannai, Khanita Suman Ham, Inhye Bu, Youngmin Kim, Hocheol Choi, Ho-Young |
author_sort | Kim, Bumjung |
collection | PubMed |
description | Ligusticum jeholense has been used as the traditional medicine ‘Go-Bon’ (Chinese name, Gao-ben) in China and Korea. Considering the increased use of medicinal herbs to treat hypertension, in this study, we aimed to investigate the mechanisms of the vasorelaxation effect caused by L. jeholense. We tested the methanol (MeOH) extract of L. jeholense root and rhizoma for vasorelaxant effects; while using an isolated organ-chamber technique, L. jeholense extract (LJE) induced relaxation in the rat aortic rings by stimulating vascular endothelial and smooth muscle cells. LJE showed concentration-dependent relaxant effects on endothelium-intact and endothelium-denuded aortic rings pre-contracted with both phenylephrine (PE) and potassium chloride (KCl) in Krebs-Henseleit (KH) buffer. The vasorelaxant effect of LJE was partly attenuated by pre-treatment with glibenclamide or 4-aminopyridine (4-AP) as K(+) channel blockers. Moreover, LJE showed concentration-dependent inhibition of vasoconstriction by Ca(2+) supplementation in the aortic rings that were pre-contracted with PE or KCl in Ca(2+)-free KH buffer. In addition, a combination of LJE and nifedipine, pre-incubated further, decreased PE-induced contractions. The results suggested that LJE-induced vasorelaxation were related to blocking K(+) channels and inhibiting entry of extracellular Ca(2+) via receptor-operative Ca(2+) channels (ROCCs) or voltage-dependent Ca(2+) channels (VDCCs). |
format | Online Article Text |
id | pubmed-6272402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62724022018-12-31 Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta Kim, Bumjung Lee, Kyungjin Chinannai, Khanita Suman Ham, Inhye Bu, Youngmin Kim, Hocheol Choi, Ho-Young Molecules Article Ligusticum jeholense has been used as the traditional medicine ‘Go-Bon’ (Chinese name, Gao-ben) in China and Korea. Considering the increased use of medicinal herbs to treat hypertension, in this study, we aimed to investigate the mechanisms of the vasorelaxation effect caused by L. jeholense. We tested the methanol (MeOH) extract of L. jeholense root and rhizoma for vasorelaxant effects; while using an isolated organ-chamber technique, L. jeholense extract (LJE) induced relaxation in the rat aortic rings by stimulating vascular endothelial and smooth muscle cells. LJE showed concentration-dependent relaxant effects on endothelium-intact and endothelium-denuded aortic rings pre-contracted with both phenylephrine (PE) and potassium chloride (KCl) in Krebs-Henseleit (KH) buffer. The vasorelaxant effect of LJE was partly attenuated by pre-treatment with glibenclamide or 4-aminopyridine (4-AP) as K(+) channel blockers. Moreover, LJE showed concentration-dependent inhibition of vasoconstriction by Ca(2+) supplementation in the aortic rings that were pre-contracted with PE or KCl in Ca(2+)-free KH buffer. In addition, a combination of LJE and nifedipine, pre-incubated further, decreased PE-induced contractions. The results suggested that LJE-induced vasorelaxation were related to blocking K(+) channels and inhibiting entry of extracellular Ca(2+) via receptor-operative Ca(2+) channels (ROCCs) or voltage-dependent Ca(2+) channels (VDCCs). MDPI 2015-06-10 /pmc/articles/PMC6272402/ /pubmed/26065836 http://dx.doi.org/10.3390/molecules200610721 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Bumjung Lee, Kyungjin Chinannai, Khanita Suman Ham, Inhye Bu, Youngmin Kim, Hocheol Choi, Ho-Young Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta |
title | Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta |
title_full | Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta |
title_fullStr | Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta |
title_full_unstemmed | Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta |
title_short | Endothelium-Independent Vasorelaxant Effect of Ligusticum jeholense Root and Rhizoma on Rat Thoracic Aorta |
title_sort | endothelium-independent vasorelaxant effect of ligusticum jeholense root and rhizoma on rat thoracic aorta |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272402/ https://www.ncbi.nlm.nih.gov/pubmed/26065836 http://dx.doi.org/10.3390/molecules200610721 |
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