A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines

A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions in M...

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Autores principales: Lambert, Marine, Meudec, Emmanuelle, Verbaere, Arnaud, Mazerolles, Gérard, Wirth, Jérémie, Masson, Gilles, Cheynier, Véronique, Sommerer, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272418/
https://www.ncbi.nlm.nih.gov/pubmed/25942371
http://dx.doi.org/10.3390/molecules20057890
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author Lambert, Marine
Meudec, Emmanuelle
Verbaere, Arnaud
Mazerolles, Gérard
Wirth, Jérémie
Masson, Gilles
Cheynier, Véronique
Sommerer, Nicolas
author_facet Lambert, Marine
Meudec, Emmanuelle
Verbaere, Arnaud
Mazerolles, Gérard
Wirth, Jérémie
Masson, Gilles
Cheynier, Véronique
Sommerer, Nicolas
author_sort Lambert, Marine
collection PubMed
description A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, the present method allows the selective quantification of up to 152 phenolic and two additional non-phenolic wine compounds in 30 min without sample purification or pre-concentration, even at low concentration levels. This method was repeatably applied to a set of 12 rosé wines and thus proved to be suitable for high-throughput and large-scale metabolomics studies.
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spelling pubmed-62724182019-01-07 A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines Lambert, Marine Meudec, Emmanuelle Verbaere, Arnaud Mazerolles, Gérard Wirth, Jérémie Masson, Gilles Cheynier, Véronique Sommerer, Nicolas Molecules Article A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, the present method allows the selective quantification of up to 152 phenolic and two additional non-phenolic wine compounds in 30 min without sample purification or pre-concentration, even at low concentration levels. This method was repeatably applied to a set of 12 rosé wines and thus proved to be suitable for high-throughput and large-scale metabolomics studies. MDPI 2015-04-30 /pmc/articles/PMC6272418/ /pubmed/25942371 http://dx.doi.org/10.3390/molecules20057890 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lambert, Marine
Meudec, Emmanuelle
Verbaere, Arnaud
Mazerolles, Gérard
Wirth, Jérémie
Masson, Gilles
Cheynier, Véronique
Sommerer, Nicolas
A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines
title A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines
title_full A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines
title_fullStr A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines
title_full_unstemmed A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines
title_short A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines
title_sort high-throughput uhplc-qqq-ms method for polyphenol profiling in rosé wines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272418/
https://www.ncbi.nlm.nih.gov/pubmed/25942371
http://dx.doi.org/10.3390/molecules20057890
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