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An Improved HPLC-DAD Method for Quantitative Comparisons of Triterpenes in Ganoderma lucidum and Its Five Related Species Originating from Vietnam

An HPLC-DAD method for the quality control of wild and cultivated Ganoderma lucidum (Linhzhi) and related species samples was developed and validated. The quantitative determination of G. lucidum and its related species using 14 triterpene constituents, including nine ganoderma acids (compounds 4–12...

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Autores principales: Ha, Do Thi, Loan, Le Thi, Hung, Tran Manh, Han, Le Vu Ngoc, Khoi, Nguyen Minh, Dung, Le Viet, Min, Byung Sun, Nguyen, Nguyen Phuong Dai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272446/
https://www.ncbi.nlm.nih.gov/pubmed/25584835
http://dx.doi.org/10.3390/molecules20011059
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author Ha, Do Thi
Loan, Le Thi
Hung, Tran Manh
Han, Le Vu Ngoc
Khoi, Nguyen Minh
Dung, Le Viet
Min, Byung Sun
Nguyen, Nguyen Phuong Dai
author_facet Ha, Do Thi
Loan, Le Thi
Hung, Tran Manh
Han, Le Vu Ngoc
Khoi, Nguyen Minh
Dung, Le Viet
Min, Byung Sun
Nguyen, Nguyen Phuong Dai
author_sort Ha, Do Thi
collection PubMed
description An HPLC-DAD method for the quality control of wild and cultivated Ganoderma lucidum (Linhzhi) and related species samples was developed and validated. The quantitative determination of G. lucidum and its related species using 14 triterpene constituents, including nine ganoderma acids (compounds 4–12), four alcohols (compounds 13–16), and one sterol (ergosterol, 17) were reported. The standard curves were linear over the concentration range of 7.5–180 µg/mL. The LOD and LOQ values for the analyses varied from 0.34 to 1.41 µg/mL and from 1.01 to 4.23 µg/mL, respectively. The percentage recovery of each reference compound was found to be from 97.09% to 100.79%, and the RSD (%) was less than 2.35%. The precision and accuracy ranged from 0.81%–3.20% and 95.38%–102.19% for intra-day, and from 0.43%–3.67% and 96.63%–103.09% for inter-day, respectively. The study disclosed in detail significant differences between the quantities of analyzed compounds in different samples. The total triterpenes in wild Linhzhi samples were significantly higher than in cultivated ones. The total constituent contents of the five related Linhzhi samples were considerably lower than that in the G. lucidum specimens, except for G. australe as its constituent content outweighed wild Linhzhi’s content by 4:1.
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spelling pubmed-62724462018-12-28 An Improved HPLC-DAD Method for Quantitative Comparisons of Triterpenes in Ganoderma lucidum and Its Five Related Species Originating from Vietnam Ha, Do Thi Loan, Le Thi Hung, Tran Manh Han, Le Vu Ngoc Khoi, Nguyen Minh Dung, Le Viet Min, Byung Sun Nguyen, Nguyen Phuong Dai Molecules Article An HPLC-DAD method for the quality control of wild and cultivated Ganoderma lucidum (Linhzhi) and related species samples was developed and validated. The quantitative determination of G. lucidum and its related species using 14 triterpene constituents, including nine ganoderma acids (compounds 4–12), four alcohols (compounds 13–16), and one sterol (ergosterol, 17) were reported. The standard curves were linear over the concentration range of 7.5–180 µg/mL. The LOD and LOQ values for the analyses varied from 0.34 to 1.41 µg/mL and from 1.01 to 4.23 µg/mL, respectively. The percentage recovery of each reference compound was found to be from 97.09% to 100.79%, and the RSD (%) was less than 2.35%. The precision and accuracy ranged from 0.81%–3.20% and 95.38%–102.19% for intra-day, and from 0.43%–3.67% and 96.63%–103.09% for inter-day, respectively. The study disclosed in detail significant differences between the quantities of analyzed compounds in different samples. The total triterpenes in wild Linhzhi samples were significantly higher than in cultivated ones. The total constituent contents of the five related Linhzhi samples were considerably lower than that in the G. lucidum specimens, except for G. australe as its constituent content outweighed wild Linhzhi’s content by 4:1. MDPI 2015-01-09 /pmc/articles/PMC6272446/ /pubmed/25584835 http://dx.doi.org/10.3390/molecules20011059 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ha, Do Thi
Loan, Le Thi
Hung, Tran Manh
Han, Le Vu Ngoc
Khoi, Nguyen Minh
Dung, Le Viet
Min, Byung Sun
Nguyen, Nguyen Phuong Dai
An Improved HPLC-DAD Method for Quantitative Comparisons of Triterpenes in Ganoderma lucidum and Its Five Related Species Originating from Vietnam
title An Improved HPLC-DAD Method for Quantitative Comparisons of Triterpenes in Ganoderma lucidum and Its Five Related Species Originating from Vietnam
title_full An Improved HPLC-DAD Method for Quantitative Comparisons of Triterpenes in Ganoderma lucidum and Its Five Related Species Originating from Vietnam
title_fullStr An Improved HPLC-DAD Method for Quantitative Comparisons of Triterpenes in Ganoderma lucidum and Its Five Related Species Originating from Vietnam
title_full_unstemmed An Improved HPLC-DAD Method for Quantitative Comparisons of Triterpenes in Ganoderma lucidum and Its Five Related Species Originating from Vietnam
title_short An Improved HPLC-DAD Method for Quantitative Comparisons of Triterpenes in Ganoderma lucidum and Its Five Related Species Originating from Vietnam
title_sort improved hplc-dad method for quantitative comparisons of triterpenes in ganoderma lucidum and its five related species originating from vietnam
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272446/
https://www.ncbi.nlm.nih.gov/pubmed/25584835
http://dx.doi.org/10.3390/molecules20011059
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