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Secondary Metabolite Localization by Autofluorescence in Living Plant Cells
Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localizatio...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272479/ https://www.ncbi.nlm.nih.gov/pubmed/25808147 http://dx.doi.org/10.3390/molecules20035024 |
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author | Talamond, Pascale Verdeil, Jean-Luc Conéjéro, Geneviève |
author_facet | Talamond, Pascale Verdeil, Jean-Luc Conéjéro, Geneviève |
author_sort | Talamond, Pascale |
collection | PubMed |
description | Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localization of the endogenous fluorophores in living plant cells. This method, which requires no previous treatment, provides an effective experimental tool for discriminating between multiple naturally-occurring fluorophores in living-tissues. Combined with advanced Linear Unmixing, the spectral analysis extends the possibilities and enables the simultaneous detection of fluorescent molecules reliably separating overlapping emission spectra. However, as with any technology, the possibility for artifactual results does exist. This methodological article presents an overview of the applications of tissular and intra-cellular localization of these intrinsic fluorophores in leaves and fruits (here for coffee and vanilla). This method will provide new opportunities for studying cellular environments and the behavior of endogenous fluorophores in the intracellular environment. |
format | Online Article Text |
id | pubmed-6272479 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62724792018-12-31 Secondary Metabolite Localization by Autofluorescence in Living Plant Cells Talamond, Pascale Verdeil, Jean-Luc Conéjéro, Geneviève Molecules Article Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localization of the endogenous fluorophores in living plant cells. This method, which requires no previous treatment, provides an effective experimental tool for discriminating between multiple naturally-occurring fluorophores in living-tissues. Combined with advanced Linear Unmixing, the spectral analysis extends the possibilities and enables the simultaneous detection of fluorescent molecules reliably separating overlapping emission spectra. However, as with any technology, the possibility for artifactual results does exist. This methodological article presents an overview of the applications of tissular and intra-cellular localization of these intrinsic fluorophores in leaves and fruits (here for coffee and vanilla). This method will provide new opportunities for studying cellular environments and the behavior of endogenous fluorophores in the intracellular environment. MDPI 2015-03-19 /pmc/articles/PMC6272479/ /pubmed/25808147 http://dx.doi.org/10.3390/molecules20035024 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Talamond, Pascale Verdeil, Jean-Luc Conéjéro, Geneviève Secondary Metabolite Localization by Autofluorescence in Living Plant Cells |
title | Secondary Metabolite Localization by Autofluorescence in Living Plant Cells |
title_full | Secondary Metabolite Localization by Autofluorescence in Living Plant Cells |
title_fullStr | Secondary Metabolite Localization by Autofluorescence in Living Plant Cells |
title_full_unstemmed | Secondary Metabolite Localization by Autofluorescence in Living Plant Cells |
title_short | Secondary Metabolite Localization by Autofluorescence in Living Plant Cells |
title_sort | secondary metabolite localization by autofluorescence in living plant cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272479/ https://www.ncbi.nlm.nih.gov/pubmed/25808147 http://dx.doi.org/10.3390/molecules20035024 |
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