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Secondary Metabolite Localization by Autofluorescence in Living Plant Cells

Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localizatio...

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Detalles Bibliográficos
Autores principales: Talamond, Pascale, Verdeil, Jean-Luc, Conéjéro, Geneviève
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272479/
https://www.ncbi.nlm.nih.gov/pubmed/25808147
http://dx.doi.org/10.3390/molecules20035024
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author Talamond, Pascale
Verdeil, Jean-Luc
Conéjéro, Geneviève
author_facet Talamond, Pascale
Verdeil, Jean-Luc
Conéjéro, Geneviève
author_sort Talamond, Pascale
collection PubMed
description Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localization of the endogenous fluorophores in living plant cells. This method, which requires no previous treatment, provides an effective experimental tool for discriminating between multiple naturally-occurring fluorophores in living-tissues. Combined with advanced Linear Unmixing, the spectral analysis extends the possibilities and enables the simultaneous detection of fluorescent molecules reliably separating overlapping emission spectra. However, as with any technology, the possibility for artifactual results does exist. This methodological article presents an overview of the applications of tissular and intra-cellular localization of these intrinsic fluorophores in leaves and fruits (here for coffee and vanilla). This method will provide new opportunities for studying cellular environments and the behavior of endogenous fluorophores in the intracellular environment.
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spelling pubmed-62724792018-12-31 Secondary Metabolite Localization by Autofluorescence in Living Plant Cells Talamond, Pascale Verdeil, Jean-Luc Conéjéro, Geneviève Molecules Article Autofluorescent molecules are abundant in plant cells and spectral images offer means for analyzing their spectra, yielding information on their accumulation and function. Based on their fluorescence characteristics, an imaging approach using multiphoton microscopy was designed to assess localization of the endogenous fluorophores in living plant cells. This method, which requires no previous treatment, provides an effective experimental tool for discriminating between multiple naturally-occurring fluorophores in living-tissues. Combined with advanced Linear Unmixing, the spectral analysis extends the possibilities and enables the simultaneous detection of fluorescent molecules reliably separating overlapping emission spectra. However, as with any technology, the possibility for artifactual results does exist. This methodological article presents an overview of the applications of tissular and intra-cellular localization of these intrinsic fluorophores in leaves and fruits (here for coffee and vanilla). This method will provide new opportunities for studying cellular environments and the behavior of endogenous fluorophores in the intracellular environment. MDPI 2015-03-19 /pmc/articles/PMC6272479/ /pubmed/25808147 http://dx.doi.org/10.3390/molecules20035024 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Talamond, Pascale
Verdeil, Jean-Luc
Conéjéro, Geneviève
Secondary Metabolite Localization by Autofluorescence in Living Plant Cells
title Secondary Metabolite Localization by Autofluorescence in Living Plant Cells
title_full Secondary Metabolite Localization by Autofluorescence in Living Plant Cells
title_fullStr Secondary Metabolite Localization by Autofluorescence in Living Plant Cells
title_full_unstemmed Secondary Metabolite Localization by Autofluorescence in Living Plant Cells
title_short Secondary Metabolite Localization by Autofluorescence in Living Plant Cells
title_sort secondary metabolite localization by autofluorescence in living plant cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272479/
https://www.ncbi.nlm.nih.gov/pubmed/25808147
http://dx.doi.org/10.3390/molecules20035024
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