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Untargeted NMR-Based Methodology in the Study of Fruit Metabolites

In this review, fundamental aspects of the untargeted NMR-based methodology applied to fruit characterization are described. The strategy to perform the structure elucidation of fruit metabolites is discussed with some examples of spectral assignments by 2D experiments. Primary ubiquitous metabolite...

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Detalles Bibliográficos
Autores principales: Sobolev, Anatoly Petrovich, Mannina, Luisa, Proietti, Noemi, Carradori, Simone, Daglia, Maria, Giusti, Anna Maria, Antiochia, Riccarda, Capitani, Donatella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272596/
https://www.ncbi.nlm.nih.gov/pubmed/25749679
http://dx.doi.org/10.3390/molecules20034088
Descripción
Sumario:In this review, fundamental aspects of the untargeted NMR-based methodology applied to fruit characterization are described. The strategy to perform the structure elucidation of fruit metabolites is discussed with some examples of spectral assignments by 2D experiments. Primary ubiquitous metabolites as well as secondary species-specific metabolites, identified in different fruits using an untargeted (1)H-NMR approach, are summarized in a comprehensive way. Crucial aspects regarding the quantitative elaboration of spectral data are also discussed. The usefulness of the NMR-based metabolic profiling was highlighted using some results regarding quality, adulteration, varieties and geographical origin of fruits and fruit-derived products such as juices.