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Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells

Carcinoma of the uterine cervix is the second most common female tumor worldwide, surpassed only by breast cancer. Natural products from seaweeds evidencing apoptotic activity have attracted a great deal of attention as new leads for alternative and complementary preventive or therapeutic anticancer...

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Autores principales: Gomes, Dayanne Lopes, Telles, Cinthia Beatrice Silva, Costa, Mariana Santana Santos Pereira, Almeida-Lima, Jailma, Costa, Leandro Silva, Keesen, Tatjana Souza Lima, Rocha, Hugo Alexandre Oliveira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272606/
https://www.ncbi.nlm.nih.gov/pubmed/25871374
http://dx.doi.org/10.3390/molecules20046573
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author Gomes, Dayanne Lopes
Telles, Cinthia Beatrice Silva
Costa, Mariana Santana Santos Pereira
Almeida-Lima, Jailma
Costa, Leandro Silva
Keesen, Tatjana Souza Lima
Rocha, Hugo Alexandre Oliveira
author_facet Gomes, Dayanne Lopes
Telles, Cinthia Beatrice Silva
Costa, Mariana Santana Santos Pereira
Almeida-Lima, Jailma
Costa, Leandro Silva
Keesen, Tatjana Souza Lima
Rocha, Hugo Alexandre Oliveira
author_sort Gomes, Dayanne Lopes
collection PubMed
description Carcinoma of the uterine cervix is the second most common female tumor worldwide, surpassed only by breast cancer. Natural products from seaweeds evidencing apoptotic activity have attracted a great deal of attention as new leads for alternative and complementary preventive or therapeutic anticancer agents. Here, methanol extracts from 13 species of tropical seaweeds (Rhodophytas, Phaeophyta and Chlorophyta) collected from the Northeast of Brazil were assessed as apoptosis-inducing agents on human cervical adenocarcinoma (HeLa). All extracts showed different levels of cytotoxicity against HeLa cells; the most potent were obtained from the brown alga Dictyota cilliolata (MEDC) and Dictyota menstrualis (MEDM). In addition, MEDC and MEDM also inhibits SiHa (cervix carcinoma) cell proliferation. Studies with these two extracts using flow cytometry and fluorescence microscopy showed that HeLa cells exposed to MEDM and MEDC exhibit morphological and biochemical changes that characterize apoptosis as shown by loss of cell viability, chromatin condensation, phosphatidylserine externalization, and sub-G1 cell cycle phase accumulation, also MEDC induces cell cycle arrest in cell cycle phase S. Moreover, the activation of caspases 3 and 9 by these extracts suggests a mitochondria-dependent apoptosis route. However, other routes cannot be ruled out. Together, these results point out the methanol extracts of the brown algae D. mentrualis and D. cilliolata as potential sources of molecules with antitumor activity.
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spelling pubmed-62726062018-12-03 Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells Gomes, Dayanne Lopes Telles, Cinthia Beatrice Silva Costa, Mariana Santana Santos Pereira Almeida-Lima, Jailma Costa, Leandro Silva Keesen, Tatjana Souza Lima Rocha, Hugo Alexandre Oliveira Molecules Article Carcinoma of the uterine cervix is the second most common female tumor worldwide, surpassed only by breast cancer. Natural products from seaweeds evidencing apoptotic activity have attracted a great deal of attention as new leads for alternative and complementary preventive or therapeutic anticancer agents. Here, methanol extracts from 13 species of tropical seaweeds (Rhodophytas, Phaeophyta and Chlorophyta) collected from the Northeast of Brazil were assessed as apoptosis-inducing agents on human cervical adenocarcinoma (HeLa). All extracts showed different levels of cytotoxicity against HeLa cells; the most potent were obtained from the brown alga Dictyota cilliolata (MEDC) and Dictyota menstrualis (MEDM). In addition, MEDC and MEDM also inhibits SiHa (cervix carcinoma) cell proliferation. Studies with these two extracts using flow cytometry and fluorescence microscopy showed that HeLa cells exposed to MEDM and MEDC exhibit morphological and biochemical changes that characterize apoptosis as shown by loss of cell viability, chromatin condensation, phosphatidylserine externalization, and sub-G1 cell cycle phase accumulation, also MEDC induces cell cycle arrest in cell cycle phase S. Moreover, the activation of caspases 3 and 9 by these extracts suggests a mitochondria-dependent apoptosis route. However, other routes cannot be ruled out. Together, these results point out the methanol extracts of the brown algae D. mentrualis and D. cilliolata as potential sources of molecules with antitumor activity. MDPI 2015-04-13 /pmc/articles/PMC6272606/ /pubmed/25871374 http://dx.doi.org/10.3390/molecules20046573 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gomes, Dayanne Lopes
Telles, Cinthia Beatrice Silva
Costa, Mariana Santana Santos Pereira
Almeida-Lima, Jailma
Costa, Leandro Silva
Keesen, Tatjana Souza Lima
Rocha, Hugo Alexandre Oliveira
Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells
title Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells
title_full Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells
title_fullStr Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells
title_full_unstemmed Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells
title_short Methanolic Extracts from Brown Seaweeds Dictyota cilliolata and Dictyota menstrualis Induce Apoptosis in Human Cervical Adenocarcinoma HeLa Cells
title_sort methanolic extracts from brown seaweeds dictyota cilliolata and dictyota menstrualis induce apoptosis in human cervical adenocarcinoma hela cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272606/
https://www.ncbi.nlm.nih.gov/pubmed/25871374
http://dx.doi.org/10.3390/molecules20046573
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