Cargando…
NKCS, a Mutant of the NK-2 Peptide, Causes Severe Distortions and Perforations in Bacterial, But Not Human Model Lipid Membranes
NKCS is an improved mutant of the bioactive peptide NK-2, which shows strong activity against Escherichia coli and low toxicity towards human cells. The different activity demonstrates the relevance of the physico-chemical nature of the target membrane for the biological effect of this peptide. We s...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272639/ https://www.ncbi.nlm.nih.gov/pubmed/25913932 http://dx.doi.org/10.3390/molecules20046941 |
_version_ | 1783377203460833280 |
---|---|
author | Ciobanasu, Corina Rzeszutek, Agnieszka Kubitscheck, Ulrich Willumeit, Regine |
author_facet | Ciobanasu, Corina Rzeszutek, Agnieszka Kubitscheck, Ulrich Willumeit, Regine |
author_sort | Ciobanasu, Corina |
collection | PubMed |
description | NKCS is an improved mutant of the bioactive peptide NK-2, which shows strong activity against Escherichia coli and low toxicity towards human cells. The different activity demonstrates the relevance of the physico-chemical nature of the target membrane for the biological effect of this peptide. We studied the effect of this potent antimicrobial peptide on model membranes by activity studies, differential scanning calorimetry, single molecule tracking and tracer efflux experiments. We found that NKCS severely distorted, penetrated and perforated model lipid membranes that resembled bacterial membranes, but not those that were similar to human cell membranes. The interactions of NKCS with phosphatidylethanolamine, which is abundant in bacterial membranes, were especially strong and are probably responsible for its antimicrobial activity. |
format | Online Article Text |
id | pubmed-6272639 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62726392018-12-03 NKCS, a Mutant of the NK-2 Peptide, Causes Severe Distortions and Perforations in Bacterial, But Not Human Model Lipid Membranes Ciobanasu, Corina Rzeszutek, Agnieszka Kubitscheck, Ulrich Willumeit, Regine Molecules Article NKCS is an improved mutant of the bioactive peptide NK-2, which shows strong activity against Escherichia coli and low toxicity towards human cells. The different activity demonstrates the relevance of the physico-chemical nature of the target membrane for the biological effect of this peptide. We studied the effect of this potent antimicrobial peptide on model membranes by activity studies, differential scanning calorimetry, single molecule tracking and tracer efflux experiments. We found that NKCS severely distorted, penetrated and perforated model lipid membranes that resembled bacterial membranes, but not those that were similar to human cell membranes. The interactions of NKCS with phosphatidylethanolamine, which is abundant in bacterial membranes, were especially strong and are probably responsible for its antimicrobial activity. MDPI 2015-04-16 /pmc/articles/PMC6272639/ /pubmed/25913932 http://dx.doi.org/10.3390/molecules20046941 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ciobanasu, Corina Rzeszutek, Agnieszka Kubitscheck, Ulrich Willumeit, Regine NKCS, a Mutant of the NK-2 Peptide, Causes Severe Distortions and Perforations in Bacterial, But Not Human Model Lipid Membranes |
title | NKCS, a Mutant of the NK-2 Peptide, Causes Severe Distortions and Perforations in Bacterial, But Not Human Model Lipid Membranes |
title_full | NKCS, a Mutant of the NK-2 Peptide, Causes Severe Distortions and Perforations in Bacterial, But Not Human Model Lipid Membranes |
title_fullStr | NKCS, a Mutant of the NK-2 Peptide, Causes Severe Distortions and Perforations in Bacterial, But Not Human Model Lipid Membranes |
title_full_unstemmed | NKCS, a Mutant of the NK-2 Peptide, Causes Severe Distortions and Perforations in Bacterial, But Not Human Model Lipid Membranes |
title_short | NKCS, a Mutant of the NK-2 Peptide, Causes Severe Distortions and Perforations in Bacterial, But Not Human Model Lipid Membranes |
title_sort | nkcs, a mutant of the nk-2 peptide, causes severe distortions and perforations in bacterial, but not human model lipid membranes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272639/ https://www.ncbi.nlm.nih.gov/pubmed/25913932 http://dx.doi.org/10.3390/molecules20046941 |
work_keys_str_mv | AT ciobanasucorina nkcsamutantofthenk2peptidecausesseveredistortionsandperforationsinbacterialbutnothumanmodellipidmembranes AT rzeszutekagnieszka nkcsamutantofthenk2peptidecausesseveredistortionsandperforationsinbacterialbutnothumanmodellipidmembranes AT kubitscheckulrich nkcsamutantofthenk2peptidecausesseveredistortionsandperforationsinbacterialbutnothumanmodellipidmembranes AT willumeitregine nkcsamutantofthenk2peptidecausesseveredistortionsandperforationsinbacterialbutnothumanmodellipidmembranes |