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Combinational Treatment of Curcumin and Quercetin against Gastric Cancer MGC-803 Cells in Vitro
Gastric cancer remains a major health problem worldwide. Natural products, with stronger antitumor activity and fewer side effects, are potential candidates for pharmaceutical development as anticancer agents. In this study, quercetin and curcumin were chosen for testing and were applied separately...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272649/ https://www.ncbi.nlm.nih.gov/pubmed/26111180 http://dx.doi.org/10.3390/molecules200611524 |
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author | Zhang, Jian-Ye Lin, Min-Ting Zhou, Meng-Jia Yi, Tao Tang, Yi-Na Tang, Si-Li Yang, Zhi-Jun Zhao, Zhong-Zhen Chen, Hu-Biao |
author_facet | Zhang, Jian-Ye Lin, Min-Ting Zhou, Meng-Jia Yi, Tao Tang, Yi-Na Tang, Si-Li Yang, Zhi-Jun Zhao, Zhong-Zhen Chen, Hu-Biao |
author_sort | Zhang, Jian-Ye |
collection | PubMed |
description | Gastric cancer remains a major health problem worldwide. Natural products, with stronger antitumor activity and fewer side effects, are potential candidates for pharmaceutical development as anticancer agents. In this study, quercetin and curcumin were chosen for testing and were applied separately and in combination to human gastric cancer MGC-803 cells. The MTT assay was used to evaluate cell growth inhibition. Annexin V-FITC/PI was carried out to measure apoptosis rate. Flow cytometry was performed to analyze mitochondrial membrane potential levels. Western blots were applied to detect expression of cytochrome c, total and phosphorylated ERK and AKT. Combined treatment with curcumin and quercetin resulted in significant inhibition of cell proliferation, accompanied by loss of mitochondrial membrane potential (ΔΨm), release of cytochrome c and decreased phosphorylation of AKT and ERK. These results indicate that the combination of curcumin and quercetin induces apoptosis through the mitochondrial pathway. Notably, effect of combined treatment with curcumin and quercetin on gastric cancer MGC-803 cells is stronger than that of individual treatment, indicating that curcumin and quercetin combinations have potential as anti-gastric cancer drugs for further development. |
format | Online Article Text |
id | pubmed-6272649 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62726492018-12-31 Combinational Treatment of Curcumin and Quercetin against Gastric Cancer MGC-803 Cells in Vitro Zhang, Jian-Ye Lin, Min-Ting Zhou, Meng-Jia Yi, Tao Tang, Yi-Na Tang, Si-Li Yang, Zhi-Jun Zhao, Zhong-Zhen Chen, Hu-Biao Molecules Article Gastric cancer remains a major health problem worldwide. Natural products, with stronger antitumor activity and fewer side effects, are potential candidates for pharmaceutical development as anticancer agents. In this study, quercetin and curcumin were chosen for testing and were applied separately and in combination to human gastric cancer MGC-803 cells. The MTT assay was used to evaluate cell growth inhibition. Annexin V-FITC/PI was carried out to measure apoptosis rate. Flow cytometry was performed to analyze mitochondrial membrane potential levels. Western blots were applied to detect expression of cytochrome c, total and phosphorylated ERK and AKT. Combined treatment with curcumin and quercetin resulted in significant inhibition of cell proliferation, accompanied by loss of mitochondrial membrane potential (ΔΨm), release of cytochrome c and decreased phosphorylation of AKT and ERK. These results indicate that the combination of curcumin and quercetin induces apoptosis through the mitochondrial pathway. Notably, effect of combined treatment with curcumin and quercetin on gastric cancer MGC-803 cells is stronger than that of individual treatment, indicating that curcumin and quercetin combinations have potential as anti-gastric cancer drugs for further development. MDPI 2015-06-22 /pmc/articles/PMC6272649/ /pubmed/26111180 http://dx.doi.org/10.3390/molecules200611524 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Jian-Ye Lin, Min-Ting Zhou, Meng-Jia Yi, Tao Tang, Yi-Na Tang, Si-Li Yang, Zhi-Jun Zhao, Zhong-Zhen Chen, Hu-Biao Combinational Treatment of Curcumin and Quercetin against Gastric Cancer MGC-803 Cells in Vitro |
title | Combinational Treatment of Curcumin and Quercetin against Gastric Cancer MGC-803 Cells in Vitro |
title_full | Combinational Treatment of Curcumin and Quercetin against Gastric Cancer MGC-803 Cells in Vitro |
title_fullStr | Combinational Treatment of Curcumin and Quercetin against Gastric Cancer MGC-803 Cells in Vitro |
title_full_unstemmed | Combinational Treatment of Curcumin and Quercetin against Gastric Cancer MGC-803 Cells in Vitro |
title_short | Combinational Treatment of Curcumin and Quercetin against Gastric Cancer MGC-803 Cells in Vitro |
title_sort | combinational treatment of curcumin and quercetin against gastric cancer mgc-803 cells in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272649/ https://www.ncbi.nlm.nih.gov/pubmed/26111180 http://dx.doi.org/10.3390/molecules200611524 |
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