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Bacillus toyonensis Strain AEMREG6, a Bacterium Isolated from South African Marine Environment Sediment Samples Produces a Glycoprotein Bioflocculant

A bioflocculant-producing bacteria, isolated from sediment samples of a marine environment in the Eastern Cape Province of South Africa demonstrated a flocculating activity above 60% for kaolin clay suspension. Analysis of the 16S ribosomal deoxyribonucleic acid (rDNA) nucleotide sequence of the iso...

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Autores principales: Okaiyeto, Kunle, Nwodo, Uchechukwu U., Mabinya, Leonard V., Okoh, Anthony I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272666/
https://www.ncbi.nlm.nih.gov/pubmed/25806549
http://dx.doi.org/10.3390/molecules20035239
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author Okaiyeto, Kunle
Nwodo, Uchechukwu U.
Mabinya, Leonard V.
Okoh, Anthony I.
author_facet Okaiyeto, Kunle
Nwodo, Uchechukwu U.
Mabinya, Leonard V.
Okoh, Anthony I.
author_sort Okaiyeto, Kunle
collection PubMed
description A bioflocculant-producing bacteria, isolated from sediment samples of a marine environment in the Eastern Cape Province of South Africa demonstrated a flocculating activity above 60% for kaolin clay suspension. Analysis of the 16S ribosomal deoxyribonucleic acid (rDNA) nucleotide sequence of the isolate in the GenBank database showed 99% similarity to Bacillus toyonensis strain BCT-7112 and it was deposited in the GenBank as Bacillus toyonensis strain AEMREG6 with accession number KP406731. The bacteria produced a bioflocculant (REG-6) optimally in the presence of glucose and NH(4)NO(3) as the sole carbon and nitrogen source, respectively, initial medium pH of 5 and Ca(2+) as the cation of choice. Chemical analysis showed that purified REG-6 was a glycoprotein mainly composed of polysaccharide (77.8%) and protein (11.5%). It was thermally stable and had strong flocculating activity against kaolin suspension over a wide range of pH values (3–11) with a relatively low dosage requirement of 0.1 mg/mL in the presence of Mn(2+). Fourier transform infrared spectroscopy (FTIR) revealed the presence of hydroxyl, carboxyl and amide groups preferred for flocculation. Scanning electron microscopy (SEM) revealed that bridging was the main flocculation mechanism of REG-6. The outstanding flocculating performance of REG-6 holds great potential to replace the hazardous chemical flocculants currently used in water treatment.
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spelling pubmed-62726662018-12-31 Bacillus toyonensis Strain AEMREG6, a Bacterium Isolated from South African Marine Environment Sediment Samples Produces a Glycoprotein Bioflocculant Okaiyeto, Kunle Nwodo, Uchechukwu U. Mabinya, Leonard V. Okoh, Anthony I. Molecules Article A bioflocculant-producing bacteria, isolated from sediment samples of a marine environment in the Eastern Cape Province of South Africa demonstrated a flocculating activity above 60% for kaolin clay suspension. Analysis of the 16S ribosomal deoxyribonucleic acid (rDNA) nucleotide sequence of the isolate in the GenBank database showed 99% similarity to Bacillus toyonensis strain BCT-7112 and it was deposited in the GenBank as Bacillus toyonensis strain AEMREG6 with accession number KP406731. The bacteria produced a bioflocculant (REG-6) optimally in the presence of glucose and NH(4)NO(3) as the sole carbon and nitrogen source, respectively, initial medium pH of 5 and Ca(2+) as the cation of choice. Chemical analysis showed that purified REG-6 was a glycoprotein mainly composed of polysaccharide (77.8%) and protein (11.5%). It was thermally stable and had strong flocculating activity against kaolin suspension over a wide range of pH values (3–11) with a relatively low dosage requirement of 0.1 mg/mL in the presence of Mn(2+). Fourier transform infrared spectroscopy (FTIR) revealed the presence of hydroxyl, carboxyl and amide groups preferred for flocculation. Scanning electron microscopy (SEM) revealed that bridging was the main flocculation mechanism of REG-6. The outstanding flocculating performance of REG-6 holds great potential to replace the hazardous chemical flocculants currently used in water treatment. MDPI 2015-03-23 /pmc/articles/PMC6272666/ /pubmed/25806549 http://dx.doi.org/10.3390/molecules20035239 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Okaiyeto, Kunle
Nwodo, Uchechukwu U.
Mabinya, Leonard V.
Okoh, Anthony I.
Bacillus toyonensis Strain AEMREG6, a Bacterium Isolated from South African Marine Environment Sediment Samples Produces a Glycoprotein Bioflocculant
title Bacillus toyonensis Strain AEMREG6, a Bacterium Isolated from South African Marine Environment Sediment Samples Produces a Glycoprotein Bioflocculant
title_full Bacillus toyonensis Strain AEMREG6, a Bacterium Isolated from South African Marine Environment Sediment Samples Produces a Glycoprotein Bioflocculant
title_fullStr Bacillus toyonensis Strain AEMREG6, a Bacterium Isolated from South African Marine Environment Sediment Samples Produces a Glycoprotein Bioflocculant
title_full_unstemmed Bacillus toyonensis Strain AEMREG6, a Bacterium Isolated from South African Marine Environment Sediment Samples Produces a Glycoprotein Bioflocculant
title_short Bacillus toyonensis Strain AEMREG6, a Bacterium Isolated from South African Marine Environment Sediment Samples Produces a Glycoprotein Bioflocculant
title_sort bacillus toyonensis strain aemreg6, a bacterium isolated from south african marine environment sediment samples produces a glycoprotein bioflocculant
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272666/
https://www.ncbi.nlm.nih.gov/pubmed/25806549
http://dx.doi.org/10.3390/molecules20035239
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