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Simultaneous Determination of Five Components in Rat Plasma by UPLC–MS/MS and Its Application to a Comparative Pharmacokinetic Study in Baihe Zhimu Tang and Zhimu Extract

Baihe Zhimu Tang (BZT) is a famous traditional Chinese medicine recipe to treat dry coughing due to yin deficiency and for moisturizing the lungs. Zhimu is an essential ingredient in BZT used to treat inflammation, fever and diabetes. The most important active components in Zhimu are flavonoids such...

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Autores principales: Li, Guolong, Tang, Zhishu, Yang, Jie, Duan, Jinao, Qian, Dawei, Guo, Jianming, Zhu, Zhenhua, Liu, Hongbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272737/
https://www.ncbi.nlm.nih.gov/pubmed/25884551
http://dx.doi.org/10.3390/molecules20046700
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author Li, Guolong
Tang, Zhishu
Yang, Jie
Duan, Jinao
Qian, Dawei
Guo, Jianming
Zhu, Zhenhua
Liu, Hongbo
author_facet Li, Guolong
Tang, Zhishu
Yang, Jie
Duan, Jinao
Qian, Dawei
Guo, Jianming
Zhu, Zhenhua
Liu, Hongbo
author_sort Li, Guolong
collection PubMed
description Baihe Zhimu Tang (BZT) is a famous traditional Chinese medicine recipe to treat dry coughing due to yin deficiency and for moisturizing the lungs. Zhimu is an essential ingredient in BZT used to treat inflammation, fever and diabetes. The most important active components in Zhimu are flavonoids such as neomangiferin, mangiferin, and steroid saponins (e.g., timosaponin BII, anemarsaponin BIII, timosaponin AIII). The aim of this study was to compare the pharmacokinetics of mangiferin, neomangiferin, timosaponin BII, anemarsaponin BIII and timosaponin AIII in rat plasma after oral administration of BZT and Zhimu extract (ZME). A sensitive, reliable and robust LC-MS/MS method to simultaneously determine steroid saponins and flavonoids in rat plasma was successfully validated. Significant differences (p < 0.05) were found in the pharmacokinetic parameters of timosaponin BII, anemarsaponin BIII and timosaponin AIII between BZT and ZME. It was surmised that formula compatibility could significantly influence the pharmacokinetics of BZT and our study is the first to study the administration of BZT based on pharmacokinetic studies.
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spelling pubmed-62727372018-12-03 Simultaneous Determination of Five Components in Rat Plasma by UPLC–MS/MS and Its Application to a Comparative Pharmacokinetic Study in Baihe Zhimu Tang and Zhimu Extract Li, Guolong Tang, Zhishu Yang, Jie Duan, Jinao Qian, Dawei Guo, Jianming Zhu, Zhenhua Liu, Hongbo Molecules Article Baihe Zhimu Tang (BZT) is a famous traditional Chinese medicine recipe to treat dry coughing due to yin deficiency and for moisturizing the lungs. Zhimu is an essential ingredient in BZT used to treat inflammation, fever and diabetes. The most important active components in Zhimu are flavonoids such as neomangiferin, mangiferin, and steroid saponins (e.g., timosaponin BII, anemarsaponin BIII, timosaponin AIII). The aim of this study was to compare the pharmacokinetics of mangiferin, neomangiferin, timosaponin BII, anemarsaponin BIII and timosaponin AIII in rat plasma after oral administration of BZT and Zhimu extract (ZME). A sensitive, reliable and robust LC-MS/MS method to simultaneously determine steroid saponins and flavonoids in rat plasma was successfully validated. Significant differences (p < 0.05) were found in the pharmacokinetic parameters of timosaponin BII, anemarsaponin BIII and timosaponin AIII between BZT and ZME. It was surmised that formula compatibility could significantly influence the pharmacokinetics of BZT and our study is the first to study the administration of BZT based on pharmacokinetic studies. MDPI 2015-04-15 /pmc/articles/PMC6272737/ /pubmed/25884551 http://dx.doi.org/10.3390/molecules20046700 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Guolong
Tang, Zhishu
Yang, Jie
Duan, Jinao
Qian, Dawei
Guo, Jianming
Zhu, Zhenhua
Liu, Hongbo
Simultaneous Determination of Five Components in Rat Plasma by UPLC–MS/MS and Its Application to a Comparative Pharmacokinetic Study in Baihe Zhimu Tang and Zhimu Extract
title Simultaneous Determination of Five Components in Rat Plasma by UPLC–MS/MS and Its Application to a Comparative Pharmacokinetic Study in Baihe Zhimu Tang and Zhimu Extract
title_full Simultaneous Determination of Five Components in Rat Plasma by UPLC–MS/MS and Its Application to a Comparative Pharmacokinetic Study in Baihe Zhimu Tang and Zhimu Extract
title_fullStr Simultaneous Determination of Five Components in Rat Plasma by UPLC–MS/MS and Its Application to a Comparative Pharmacokinetic Study in Baihe Zhimu Tang and Zhimu Extract
title_full_unstemmed Simultaneous Determination of Five Components in Rat Plasma by UPLC–MS/MS and Its Application to a Comparative Pharmacokinetic Study in Baihe Zhimu Tang and Zhimu Extract
title_short Simultaneous Determination of Five Components in Rat Plasma by UPLC–MS/MS and Its Application to a Comparative Pharmacokinetic Study in Baihe Zhimu Tang and Zhimu Extract
title_sort simultaneous determination of five components in rat plasma by uplc–ms/ms and its application to a comparative pharmacokinetic study in baihe zhimu tang and zhimu extract
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272737/
https://www.ncbi.nlm.nih.gov/pubmed/25884551
http://dx.doi.org/10.3390/molecules20046700
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