Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolor

An 86-kDa homodimeric angiotensin I-converting enzyme (ACE) inhibitory protein designated as LTP was isolated from fruit bodies of the mushroom Leucopaxillus tricolor. The isolation procedure involved ultrafiltration through a membrane with a molecular weight cutoff of 10-kDa, ion exchange chromatog...

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Autores principales: Geng, Xueran, Tian, Guoting, Zhang, Weiwei, Zhao, Yongchang, Zhao, Liyan, Ryu, Mansok, Wang, Hexiang, Ng, Tzi Bun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272794/
https://www.ncbi.nlm.nih.gov/pubmed/26039337
http://dx.doi.org/10.3390/molecules200610141
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author Geng, Xueran
Tian, Guoting
Zhang, Weiwei
Zhao, Yongchang
Zhao, Liyan
Ryu, Mansok
Wang, Hexiang
Ng, Tzi Bun
author_facet Geng, Xueran
Tian, Guoting
Zhang, Weiwei
Zhao, Yongchang
Zhao, Liyan
Ryu, Mansok
Wang, Hexiang
Ng, Tzi Bun
author_sort Geng, Xueran
collection PubMed
description An 86-kDa homodimeric angiotensin I-converting enzyme (ACE) inhibitory protein designated as LTP was isolated from fruit bodies of the mushroom Leucopaxillus tricolor. The isolation procedure involved ultrafiltration through a membrane with a molecular weight cutoff of 10-kDa, ion exchange chromatography on Q-Sepharose, and finally fast protein liquid chromatography-gel filtration on Superdex 75. LTP exhibited an IC(50) value of 1.64 mg∙mL(−1) for its ACE inhibitory activity. The unique N-terminal amino acid sequence of LTP was disclosed by Edman degradation to be DGPTMHRQAVADFKQ. In addition, seven internal sequences of LTP were elucidated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Results of the Lineweaver-Burk plot suggested that LTP competitively inhibited ACE. Both LTP and the water extract of L. tricolor exhibited a clear antihypertensive effect on spontaneously hypertensive rats.
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spelling pubmed-62727942018-12-31 Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolor Geng, Xueran Tian, Guoting Zhang, Weiwei Zhao, Yongchang Zhao, Liyan Ryu, Mansok Wang, Hexiang Ng, Tzi Bun Molecules Article An 86-kDa homodimeric angiotensin I-converting enzyme (ACE) inhibitory protein designated as LTP was isolated from fruit bodies of the mushroom Leucopaxillus tricolor. The isolation procedure involved ultrafiltration through a membrane with a molecular weight cutoff of 10-kDa, ion exchange chromatography on Q-Sepharose, and finally fast protein liquid chromatography-gel filtration on Superdex 75. LTP exhibited an IC(50) value of 1.64 mg∙mL(−1) for its ACE inhibitory activity. The unique N-terminal amino acid sequence of LTP was disclosed by Edman degradation to be DGPTMHRQAVADFKQ. In addition, seven internal sequences of LTP were elucidated by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Results of the Lineweaver-Burk plot suggested that LTP competitively inhibited ACE. Both LTP and the water extract of L. tricolor exhibited a clear antihypertensive effect on spontaneously hypertensive rats. MDPI 2015-06-01 /pmc/articles/PMC6272794/ /pubmed/26039337 http://dx.doi.org/10.3390/molecules200610141 Text en © 2015 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Geng, Xueran
Tian, Guoting
Zhang, Weiwei
Zhao, Yongchang
Zhao, Liyan
Ryu, Mansok
Wang, Hexiang
Ng, Tzi Bun
Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolor
title Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolor
title_full Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolor
title_fullStr Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolor
title_full_unstemmed Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolor
title_short Isolation of an Angiotensin I-Converting Enzyme Inhibitory Protein with Antihypertensive Effect in Spontaneously Hypertensive Rats from the Edible Wild Mushroom Leucopaxillus tricolor
title_sort isolation of an angiotensin i-converting enzyme inhibitory protein with antihypertensive effect in spontaneously hypertensive rats from the edible wild mushroom leucopaxillus tricolor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272794/
https://www.ncbi.nlm.nih.gov/pubmed/26039337
http://dx.doi.org/10.3390/molecules200610141
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