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Simultaneous Saccharification and Fermentation of Sugar Beet Pulp with Mixed Bacterial Cultures for Lactic Acid and Propylene Glycol Production

Research into fermentative production of lactic acid from agricultural by-products has recently concentrated on the direct conversion of biomass, whereby pure sugars are replaced with inexpensive feedstock in the process of lactic acid production. In our studies, for the first time, the source of ca...

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Autores principales: Berlowska, Joanna, Cieciura, Weronika, Borowski, Sebastian, Dudkiewicz, Marta, Binczarski, Michal, Witonska, Izabela, Otlewska, Anna, Kregiel, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272889/
https://www.ncbi.nlm.nih.gov/pubmed/27763527
http://dx.doi.org/10.3390/molecules21101380
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author Berlowska, Joanna
Cieciura, Weronika
Borowski, Sebastian
Dudkiewicz, Marta
Binczarski, Michal
Witonska, Izabela
Otlewska, Anna
Kregiel, Dorota
author_facet Berlowska, Joanna
Cieciura, Weronika
Borowski, Sebastian
Dudkiewicz, Marta
Binczarski, Michal
Witonska, Izabela
Otlewska, Anna
Kregiel, Dorota
author_sort Berlowska, Joanna
collection PubMed
description Research into fermentative production of lactic acid from agricultural by-products has recently concentrated on the direct conversion of biomass, whereby pure sugars are replaced with inexpensive feedstock in the process of lactic acid production. In our studies, for the first time, the source of carbon used is sugar beet pulp, generated as a by-product of industrial sugar production. In this paper, we focus on the simultaneous saccharification of lignocellulosic biomass and fermentation of lactic acid, using mixed cultures with complementary assimilation profiles. Lactic acid is one of the primary platform chemicals, and can be used to synthesize a wide variety of useful products, including green propylene glycol. A series of controlled batch fermentations was conducted under various conditions, including pretreatment with enzymatic hydrolysis. Inoculation was performed in two sequential stages, to avoid carbon catabolite repression. Biologically-synthesized lactic acid was catalytically reduced to propylene glycol over 5% Ru/C. The highest lactic acid yield was obtained with mixed cultures. The yield of propylene glycol from the biological lactic acid was similar to that obtained with a water solution of pure lactic acid. Our results show that simultaneous saccharification and fermentation enables generation of lactic acid, suitable for further chemical transformations, from agricultural residues.
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spelling pubmed-62728892018-12-28 Simultaneous Saccharification and Fermentation of Sugar Beet Pulp with Mixed Bacterial Cultures for Lactic Acid and Propylene Glycol Production Berlowska, Joanna Cieciura, Weronika Borowski, Sebastian Dudkiewicz, Marta Binczarski, Michal Witonska, Izabela Otlewska, Anna Kregiel, Dorota Molecules Article Research into fermentative production of lactic acid from agricultural by-products has recently concentrated on the direct conversion of biomass, whereby pure sugars are replaced with inexpensive feedstock in the process of lactic acid production. In our studies, for the first time, the source of carbon used is sugar beet pulp, generated as a by-product of industrial sugar production. In this paper, we focus on the simultaneous saccharification of lignocellulosic biomass and fermentation of lactic acid, using mixed cultures with complementary assimilation profiles. Lactic acid is one of the primary platform chemicals, and can be used to synthesize a wide variety of useful products, including green propylene glycol. A series of controlled batch fermentations was conducted under various conditions, including pretreatment with enzymatic hydrolysis. Inoculation was performed in two sequential stages, to avoid carbon catabolite repression. Biologically-synthesized lactic acid was catalytically reduced to propylene glycol over 5% Ru/C. The highest lactic acid yield was obtained with mixed cultures. The yield of propylene glycol from the biological lactic acid was similar to that obtained with a water solution of pure lactic acid. Our results show that simultaneous saccharification and fermentation enables generation of lactic acid, suitable for further chemical transformations, from agricultural residues. MDPI 2016-10-17 /pmc/articles/PMC6272889/ /pubmed/27763527 http://dx.doi.org/10.3390/molecules21101380 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Berlowska, Joanna
Cieciura, Weronika
Borowski, Sebastian
Dudkiewicz, Marta
Binczarski, Michal
Witonska, Izabela
Otlewska, Anna
Kregiel, Dorota
Simultaneous Saccharification and Fermentation of Sugar Beet Pulp with Mixed Bacterial Cultures for Lactic Acid and Propylene Glycol Production
title Simultaneous Saccharification and Fermentation of Sugar Beet Pulp with Mixed Bacterial Cultures for Lactic Acid and Propylene Glycol Production
title_full Simultaneous Saccharification and Fermentation of Sugar Beet Pulp with Mixed Bacterial Cultures for Lactic Acid and Propylene Glycol Production
title_fullStr Simultaneous Saccharification and Fermentation of Sugar Beet Pulp with Mixed Bacterial Cultures for Lactic Acid and Propylene Glycol Production
title_full_unstemmed Simultaneous Saccharification and Fermentation of Sugar Beet Pulp with Mixed Bacterial Cultures for Lactic Acid and Propylene Glycol Production
title_short Simultaneous Saccharification and Fermentation of Sugar Beet Pulp with Mixed Bacterial Cultures for Lactic Acid and Propylene Glycol Production
title_sort simultaneous saccharification and fermentation of sugar beet pulp with mixed bacterial cultures for lactic acid and propylene glycol production
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272889/
https://www.ncbi.nlm.nih.gov/pubmed/27763527
http://dx.doi.org/10.3390/molecules21101380
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