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Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis
This article reviews recent developments in droplet microfluidics enabling high-throughput single-cell analysis. Five key aspects in this field are included in this review: (1) prototype demonstration of single-cell encapsulation in microfluidic droplets; (2) technical improvements of single-cell en...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272933/ https://www.ncbi.nlm.nih.gov/pubmed/27399651 http://dx.doi.org/10.3390/molecules21070881 |
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author | Wen, Na Zhao, Zhan Fan, Beiyuan Chen, Deyong Men, Dong Wang, Junbo Chen, Jian |
author_facet | Wen, Na Zhao, Zhan Fan, Beiyuan Chen, Deyong Men, Dong Wang, Junbo Chen, Jian |
author_sort | Wen, Na |
collection | PubMed |
description | This article reviews recent developments in droplet microfluidics enabling high-throughput single-cell analysis. Five key aspects in this field are included in this review: (1) prototype demonstration of single-cell encapsulation in microfluidic droplets; (2) technical improvements of single-cell encapsulation in microfluidic droplets; (3) microfluidic droplets enabling single-cell proteomic analysis; (4) microfluidic droplets enabling single-cell genomic analysis; and (5) integrated microfluidic droplet systems enabling single-cell screening. We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on key performances of throughput, multifunctionality, and absolute quantification. |
format | Online Article Text |
id | pubmed-6272933 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62729332018-12-28 Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis Wen, Na Zhao, Zhan Fan, Beiyuan Chen, Deyong Men, Dong Wang, Junbo Chen, Jian Molecules Review This article reviews recent developments in droplet microfluidics enabling high-throughput single-cell analysis. Five key aspects in this field are included in this review: (1) prototype demonstration of single-cell encapsulation in microfluidic droplets; (2) technical improvements of single-cell encapsulation in microfluidic droplets; (3) microfluidic droplets enabling single-cell proteomic analysis; (4) microfluidic droplets enabling single-cell genomic analysis; and (5) integrated microfluidic droplet systems enabling single-cell screening. We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on key performances of throughput, multifunctionality, and absolute quantification. MDPI 2016-07-05 /pmc/articles/PMC6272933/ /pubmed/27399651 http://dx.doi.org/10.3390/molecules21070881 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Wen, Na Zhao, Zhan Fan, Beiyuan Chen, Deyong Men, Dong Wang, Junbo Chen, Jian Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis |
title | Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis |
title_full | Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis |
title_fullStr | Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis |
title_full_unstemmed | Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis |
title_short | Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis |
title_sort | development of droplet microfluidics enabling high-throughput single-cell analysis |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6272933/ https://www.ncbi.nlm.nih.gov/pubmed/27399651 http://dx.doi.org/10.3390/molecules21070881 |
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