Molecular Cloning of cpcU and Heterodimeric Bilin Lyase Activity Analysis of CpcU and CpcS for Attachment of Phycocyanobilin to Cys-82 on the β-Subunit of Phycocyanin in Arthrospira platensis FACHB314

A new bilin lyase gene cpcU was cloned from Arthrospira platensis FACHB314 to study the assembly of the phycocyanin β-Subunit. Two recombinant plasmids, one contained the phycocyanobilin (PCB) producing genes (hoxI and pcyA), while the other contained the gene of the β-Subunit of phycobiliprotein (c...

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Autores principales: Wu, Fei, Zang, Xiaonan, Zhang, Xuecheng, Zhang, Ran, Huang, Xiaoyun, Hou, Lulu, Jiang, Minjie, Liu, Chang, Pang, Chunhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273044/
https://www.ncbi.nlm.nih.gov/pubmed/26999083
http://dx.doi.org/10.3390/molecules21030357
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author Wu, Fei
Zang, Xiaonan
Zhang, Xuecheng
Zhang, Ran
Huang, Xiaoyun
Hou, Lulu
Jiang, Minjie
Liu, Chang
Pang, Chunhong
author_facet Wu, Fei
Zang, Xiaonan
Zhang, Xuecheng
Zhang, Ran
Huang, Xiaoyun
Hou, Lulu
Jiang, Minjie
Liu, Chang
Pang, Chunhong
author_sort Wu, Fei
collection PubMed
description A new bilin lyase gene cpcU was cloned from Arthrospira platensis FACHB314 to study the assembly of the phycocyanin β-Subunit. Two recombinant plasmids, one contained the phycocyanobilin (PCB) producing genes (hoxI and pcyA), while the other contained the gene of the β-Subunit of phycobiliprotein (cpcB) and the lyase gene (cpcU, cpcS, or cpcU/S) were constructed and separately transferred into Escherichia coli in order to test the activities of relevant lyases for catalyzing PCB addition to CpcB during synthesizing fluorescent β-PC of A. platensis FACHB314. The fluorescence intensity examination showed that Cys-82 maybe the active site for the β-Subunit binding to PCBs and the attachment could be carried out by CpcU, CpcS, or co-expressed cpcU/S in A. platensis FACHB314.
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spelling pubmed-62730442018-12-28 Molecular Cloning of cpcU and Heterodimeric Bilin Lyase Activity Analysis of CpcU and CpcS for Attachment of Phycocyanobilin to Cys-82 on the β-Subunit of Phycocyanin in Arthrospira platensis FACHB314 Wu, Fei Zang, Xiaonan Zhang, Xuecheng Zhang, Ran Huang, Xiaoyun Hou, Lulu Jiang, Minjie Liu, Chang Pang, Chunhong Molecules Article A new bilin lyase gene cpcU was cloned from Arthrospira platensis FACHB314 to study the assembly of the phycocyanin β-Subunit. Two recombinant plasmids, one contained the phycocyanobilin (PCB) producing genes (hoxI and pcyA), while the other contained the gene of the β-Subunit of phycobiliprotein (cpcB) and the lyase gene (cpcU, cpcS, or cpcU/S) were constructed and separately transferred into Escherichia coli in order to test the activities of relevant lyases for catalyzing PCB addition to CpcB during synthesizing fluorescent β-PC of A. platensis FACHB314. The fluorescence intensity examination showed that Cys-82 maybe the active site for the β-Subunit binding to PCBs and the attachment could be carried out by CpcU, CpcS, or co-expressed cpcU/S in A. platensis FACHB314. MDPI 2016-03-16 /pmc/articles/PMC6273044/ /pubmed/26999083 http://dx.doi.org/10.3390/molecules21030357 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Fei
Zang, Xiaonan
Zhang, Xuecheng
Zhang, Ran
Huang, Xiaoyun
Hou, Lulu
Jiang, Minjie
Liu, Chang
Pang, Chunhong
Molecular Cloning of cpcU and Heterodimeric Bilin Lyase Activity Analysis of CpcU and CpcS for Attachment of Phycocyanobilin to Cys-82 on the β-Subunit of Phycocyanin in Arthrospira platensis FACHB314
title Molecular Cloning of cpcU and Heterodimeric Bilin Lyase Activity Analysis of CpcU and CpcS for Attachment of Phycocyanobilin to Cys-82 on the β-Subunit of Phycocyanin in Arthrospira platensis FACHB314
title_full Molecular Cloning of cpcU and Heterodimeric Bilin Lyase Activity Analysis of CpcU and CpcS for Attachment of Phycocyanobilin to Cys-82 on the β-Subunit of Phycocyanin in Arthrospira platensis FACHB314
title_fullStr Molecular Cloning of cpcU and Heterodimeric Bilin Lyase Activity Analysis of CpcU and CpcS for Attachment of Phycocyanobilin to Cys-82 on the β-Subunit of Phycocyanin in Arthrospira platensis FACHB314
title_full_unstemmed Molecular Cloning of cpcU and Heterodimeric Bilin Lyase Activity Analysis of CpcU and CpcS for Attachment of Phycocyanobilin to Cys-82 on the β-Subunit of Phycocyanin in Arthrospira platensis FACHB314
title_short Molecular Cloning of cpcU and Heterodimeric Bilin Lyase Activity Analysis of CpcU and CpcS for Attachment of Phycocyanobilin to Cys-82 on the β-Subunit of Phycocyanin in Arthrospira platensis FACHB314
title_sort molecular cloning of cpcu and heterodimeric bilin lyase activity analysis of cpcu and cpcs for attachment of phycocyanobilin to cys-82 on the β-subunit of phycocyanin in arthrospira platensis fachb314
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273044/
https://www.ncbi.nlm.nih.gov/pubmed/26999083
http://dx.doi.org/10.3390/molecules21030357
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