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The Traditional Chinese Medicine DangguiBuxue Tang Sensitizes Colorectal Cancer Cells to Chemoradiotherapy

Chemotherapy is an important treatment modality for colon cancer, and concurrent chemoradiation therapy (CCRT) is the preferred treatment route for patients with stage II and III rectal cancer. We examined whether DangguiBuxue Tang (DBT), a traditional Chinese herbal extract, sensitizes colorectal c...

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Autores principales: Chen, Shun-Ting, Lee, Tzung-Yan, Tsai, Tung-Hu, Lin, Yin-Cheng, Lin, Chin-Ping, Shieh, Hui-Ru, Hsu, Ming-Ling, Chi, Chih-Wen, Lee, Ming-Cheng, Chang, Hen-Hong, Chen, Yu-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273051/
https://www.ncbi.nlm.nih.gov/pubmed/27929437
http://dx.doi.org/10.3390/molecules21121677
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author Chen, Shun-Ting
Lee, Tzung-Yan
Tsai, Tung-Hu
Lin, Yin-Cheng
Lin, Chin-Ping
Shieh, Hui-Ru
Hsu, Ming-Ling
Chi, Chih-Wen
Lee, Ming-Cheng
Chang, Hen-Hong
Chen, Yu-Jen
author_facet Chen, Shun-Ting
Lee, Tzung-Yan
Tsai, Tung-Hu
Lin, Yin-Cheng
Lin, Chin-Ping
Shieh, Hui-Ru
Hsu, Ming-Ling
Chi, Chih-Wen
Lee, Ming-Cheng
Chang, Hen-Hong
Chen, Yu-Jen
author_sort Chen, Shun-Ting
collection PubMed
description Chemotherapy is an important treatment modality for colon cancer, and concurrent chemoradiation therapy (CCRT) is the preferred treatment route for patients with stage II and III rectal cancer. We examined whether DangguiBuxue Tang (DBT), a traditional Chinese herbal extract, sensitizes colorectal cancer cells to anticancer treatments. The polysaccharide-depleted fraction of DBT (DBT-PD) contains greater amounts of astragaloside IV (312.626 µg/g) and ferulic acid (1.404 µg/g) than does the original formula. Treatment of the murine colon carcinoma cell line (CT26) with DBT-PD inhibits growth, whereas treatment with comparable amounts of purified astragaloside IV and ferulic acid showed no significant effect. Concurrent treatment with DBT-PD increases the growth inhibitory effect of 5-fluorouracil up to 4.39-fold. DBT-PD enhances the effect of radiation therapy (RT) with a sensitizer enhancement ratio (SER) of up to 1.3. It also increases the therapeutic effect of CCRT on CT26 cells. Cells treated with DBP-PD showed ultrastructural changes characteristic of autophagy, including multiple cytoplasmic vacuoles with double-layered membranes, vacuoles containing remnants of degraded organelles, marked swelling and vacuolization of mitochondria, and autolysosome-like vacuoles. We conclude that DBT-PD induces autophagy-associated cell death in CT26 cells, and may have potential as a chemotherapy or radiotherapy sensitizer in colorectal cancer treatment.
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spelling pubmed-62730512018-12-28 The Traditional Chinese Medicine DangguiBuxue Tang Sensitizes Colorectal Cancer Cells to Chemoradiotherapy Chen, Shun-Ting Lee, Tzung-Yan Tsai, Tung-Hu Lin, Yin-Cheng Lin, Chin-Ping Shieh, Hui-Ru Hsu, Ming-Ling Chi, Chih-Wen Lee, Ming-Cheng Chang, Hen-Hong Chen, Yu-Jen Molecules Article Chemotherapy is an important treatment modality for colon cancer, and concurrent chemoradiation therapy (CCRT) is the preferred treatment route for patients with stage II and III rectal cancer. We examined whether DangguiBuxue Tang (DBT), a traditional Chinese herbal extract, sensitizes colorectal cancer cells to anticancer treatments. The polysaccharide-depleted fraction of DBT (DBT-PD) contains greater amounts of astragaloside IV (312.626 µg/g) and ferulic acid (1.404 µg/g) than does the original formula. Treatment of the murine colon carcinoma cell line (CT26) with DBT-PD inhibits growth, whereas treatment with comparable amounts of purified astragaloside IV and ferulic acid showed no significant effect. Concurrent treatment with DBT-PD increases the growth inhibitory effect of 5-fluorouracil up to 4.39-fold. DBT-PD enhances the effect of radiation therapy (RT) with a sensitizer enhancement ratio (SER) of up to 1.3. It also increases the therapeutic effect of CCRT on CT26 cells. Cells treated with DBP-PD showed ultrastructural changes characteristic of autophagy, including multiple cytoplasmic vacuoles with double-layered membranes, vacuoles containing remnants of degraded organelles, marked swelling and vacuolization of mitochondria, and autolysosome-like vacuoles. We conclude that DBT-PD induces autophagy-associated cell death in CT26 cells, and may have potential as a chemotherapy or radiotherapy sensitizer in colorectal cancer treatment. MDPI 2016-12-06 /pmc/articles/PMC6273051/ /pubmed/27929437 http://dx.doi.org/10.3390/molecules21121677 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Shun-Ting
Lee, Tzung-Yan
Tsai, Tung-Hu
Lin, Yin-Cheng
Lin, Chin-Ping
Shieh, Hui-Ru
Hsu, Ming-Ling
Chi, Chih-Wen
Lee, Ming-Cheng
Chang, Hen-Hong
Chen, Yu-Jen
The Traditional Chinese Medicine DangguiBuxue Tang Sensitizes Colorectal Cancer Cells to Chemoradiotherapy
title The Traditional Chinese Medicine DangguiBuxue Tang Sensitizes Colorectal Cancer Cells to Chemoradiotherapy
title_full The Traditional Chinese Medicine DangguiBuxue Tang Sensitizes Colorectal Cancer Cells to Chemoradiotherapy
title_fullStr The Traditional Chinese Medicine DangguiBuxue Tang Sensitizes Colorectal Cancer Cells to Chemoradiotherapy
title_full_unstemmed The Traditional Chinese Medicine DangguiBuxue Tang Sensitizes Colorectal Cancer Cells to Chemoradiotherapy
title_short The Traditional Chinese Medicine DangguiBuxue Tang Sensitizes Colorectal Cancer Cells to Chemoradiotherapy
title_sort traditional chinese medicine dangguibuxue tang sensitizes colorectal cancer cells to chemoradiotherapy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273051/
https://www.ncbi.nlm.nih.gov/pubmed/27929437
http://dx.doi.org/10.3390/molecules21121677
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