Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale

Genome mining is an effective tool used to discover novel natural products from actinomycetes. Genome sequence analysis of Streptomyces sp. FJS31-2 revealed the presence of one putative type II polyketide gene cluster (ABX), which may correspond to type II polyketide products including BE-24566B and...

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Autores principales: Lü, Yuhong, Yue, Changwu, Shao, Meiyun, Qian, Shengyan, Liu, Ning, Bao, Yuxin, Wang, Miao, Liu, Minghao, Li, Xiaoqian, Wang, Yinyin, Huang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273070/
https://www.ncbi.nlm.nih.gov/pubmed/27248985
http://dx.doi.org/10.3390/molecules21060711
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author Lü, Yuhong
Yue, Changwu
Shao, Meiyun
Qian, Shengyan
Liu, Ning
Bao, Yuxin
Wang, Miao
Liu, Minghao
Li, Xiaoqian
Wang, Yinyin
Huang, Ying
author_facet Lü, Yuhong
Yue, Changwu
Shao, Meiyun
Qian, Shengyan
Liu, Ning
Bao, Yuxin
Wang, Miao
Liu, Minghao
Li, Xiaoqian
Wang, Yinyin
Huang, Ying
author_sort Lü, Yuhong
collection PubMed
description Genome mining is an effective tool used to discover novel natural products from actinomycetes. Genome sequence analysis of Streptomyces sp. FJS31-2 revealed the presence of one putative type II polyketide gene cluster (ABX), which may correspond to type II polyketide products including BE-24566B and its chloro-derivatives. The addition of natural humus acid successfully activated the biosynthsis of the abx gene cluster. BE-24566B and its chloro-derivatives, named zunyimycin A, were also detected. The targeted deletion of the polyketide skeleton synthesis genes such as abxp, abxk, and abxs was performed in the wild strain to identify the gene cluster for BE-24566B biosynthesis.
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spelling pubmed-62730702018-12-28 Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale Lü, Yuhong Yue, Changwu Shao, Meiyun Qian, Shengyan Liu, Ning Bao, Yuxin Wang, Miao Liu, Minghao Li, Xiaoqian Wang, Yinyin Huang, Ying Molecules Article Genome mining is an effective tool used to discover novel natural products from actinomycetes. Genome sequence analysis of Streptomyces sp. FJS31-2 revealed the presence of one putative type II polyketide gene cluster (ABX), which may correspond to type II polyketide products including BE-24566B and its chloro-derivatives. The addition of natural humus acid successfully activated the biosynthsis of the abx gene cluster. BE-24566B and its chloro-derivatives, named zunyimycin A, were also detected. The targeted deletion of the polyketide skeleton synthesis genes such as abxp, abxk, and abxs was performed in the wild strain to identify the gene cluster for BE-24566B biosynthesis. MDPI 2016-05-30 /pmc/articles/PMC6273070/ /pubmed/27248985 http://dx.doi.org/10.3390/molecules21060711 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lü, Yuhong
Yue, Changwu
Shao, Meiyun
Qian, Shengyan
Liu, Ning
Bao, Yuxin
Wang, Miao
Liu, Minghao
Li, Xiaoqian
Wang, Yinyin
Huang, Ying
Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale
title Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale
title_full Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale
title_fullStr Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale
title_full_unstemmed Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale
title_short Molecular Genetic Characterization of an Anthrabenzoxocinones Gene Cluster in Streptomyces Sp. FJS31-2 for the Biosynthesis of BE-24566B and Zunyimycin Ale
title_sort molecular genetic characterization of an anthrabenzoxocinones gene cluster in streptomyces sp. fjs31-2 for the biosynthesis of be-24566b and zunyimycin ale
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273070/
https://www.ncbi.nlm.nih.gov/pubmed/27248985
http://dx.doi.org/10.3390/molecules21060711
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