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Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice

In this study, we found that chloroform fraction (CF) from TJP ethanolic extract inhibited lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and intracellular ROS in RAW264.7 cells. In addition, expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) genes wa...

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Autores principales: Kim, Yon-Suk, Hwang, Jin-Woo, Jang, Jae-Hyuk, Son, Sangkeun, Seo, Il-Bok, Jeong, Jae-Hyun, Kim, Ee-Hwa, Moon, Sang-Ho, Jeon, Byong-Tae, Park, Pyo-Jam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273097/
https://www.ncbi.nlm.nih.gov/pubmed/27007369
http://dx.doi.org/10.3390/molecules21030392
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author Kim, Yon-Suk
Hwang, Jin-Woo
Jang, Jae-Hyuk
Son, Sangkeun
Seo, Il-Bok
Jeong, Jae-Hyun
Kim, Ee-Hwa
Moon, Sang-Ho
Jeon, Byong-Tae
Park, Pyo-Jam
author_facet Kim, Yon-Suk
Hwang, Jin-Woo
Jang, Jae-Hyuk
Son, Sangkeun
Seo, Il-Bok
Jeong, Jae-Hyun
Kim, Ee-Hwa
Moon, Sang-Ho
Jeon, Byong-Tae
Park, Pyo-Jam
author_sort Kim, Yon-Suk
collection PubMed
description In this study, we found that chloroform fraction (CF) from TJP ethanolic extract inhibited lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and intracellular ROS in RAW264.7 cells. In addition, expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) genes was reduced, as evidenced by western blot. Our results indicate that CF exerts anti-inflammatory effects by down-regulating expression of iNOS and COX-2 genes through inhibition of MAPK (ERK, JNK and p38) and NF-κB signaling. Similarly we also evaluated the effects of CF on LPS-induced acute lung injury. Male Balb/c mice were pretreated with dexamethasone or CF 1 h before intranasal instillation of LPS. Eight hours after LPS administration, the inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The results indicated that CF inhibited LPS-induced TNF-α and IL-6 production in a dose dependent manner. It was also observed that CF attenuated LPS-induced lung histopathologic changes. In conclusion, these data demonstrate that the protective effect of CF on LPS-induced acute lung injury (ALI) in mice might relate to the suppression of excessive inflammatory responses in lung tissue. Thus, it can be suggested that CF might be a potential therapeutic agent for ALI.
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spelling pubmed-62730972018-12-28 Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice Kim, Yon-Suk Hwang, Jin-Woo Jang, Jae-Hyuk Son, Sangkeun Seo, Il-Bok Jeong, Jae-Hyun Kim, Ee-Hwa Moon, Sang-Ho Jeon, Byong-Tae Park, Pyo-Jam Molecules Article In this study, we found that chloroform fraction (CF) from TJP ethanolic extract inhibited lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and intracellular ROS in RAW264.7 cells. In addition, expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) genes was reduced, as evidenced by western blot. Our results indicate that CF exerts anti-inflammatory effects by down-regulating expression of iNOS and COX-2 genes through inhibition of MAPK (ERK, JNK and p38) and NF-κB signaling. Similarly we also evaluated the effects of CF on LPS-induced acute lung injury. Male Balb/c mice were pretreated with dexamethasone or CF 1 h before intranasal instillation of LPS. Eight hours after LPS administration, the inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The results indicated that CF inhibited LPS-induced TNF-α and IL-6 production in a dose dependent manner. It was also observed that CF attenuated LPS-induced lung histopathologic changes. In conclusion, these data demonstrate that the protective effect of CF on LPS-induced acute lung injury (ALI) in mice might relate to the suppression of excessive inflammatory responses in lung tissue. Thus, it can be suggested that CF might be a potential therapeutic agent for ALI. MDPI 2016-03-21 /pmc/articles/PMC6273097/ /pubmed/27007369 http://dx.doi.org/10.3390/molecules21030392 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Yon-Suk
Hwang, Jin-Woo
Jang, Jae-Hyuk
Son, Sangkeun
Seo, Il-Bok
Jeong, Jae-Hyun
Kim, Ee-Hwa
Moon, Sang-Ho
Jeon, Byong-Tae
Park, Pyo-Jam
Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice
title Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice
title_full Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice
title_fullStr Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice
title_full_unstemmed Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice
title_short Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice
title_sort trapa japonica pericarp extract reduces lps-induced inflammation in macrophages and acute lung injury in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273097/
https://www.ncbi.nlm.nih.gov/pubmed/27007369
http://dx.doi.org/10.3390/molecules21030392
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