Cargando…
Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats
Colloidal suspensions of 14 nm gold nanoparticles (AuNPs) were repeatedly administered intravenously at three dose levels (0.9, 9 and 90 µg) to male Sprague Dawley rats weekly for 7 weeks, followed by a 14-day washout period. After sacrificing, the amount of gold was quantified in the liver, lungs,...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273121/ https://www.ncbi.nlm.nih.gov/pubmed/27294904 http://dx.doi.org/10.3390/molecules21060763 |
_version_ | 1783377311960137728 |
---|---|
author | Rambanapasi, Clinton Zeevaart, Jan Rijn Buntting, Hylton Bester, Cornelius Kotze, Deon Hayeshi, Rose Grobler, Anne |
author_facet | Rambanapasi, Clinton Zeevaart, Jan Rijn Buntting, Hylton Bester, Cornelius Kotze, Deon Hayeshi, Rose Grobler, Anne |
author_sort | Rambanapasi, Clinton |
collection | PubMed |
description | Colloidal suspensions of 14 nm gold nanoparticles (AuNPs) were repeatedly administered intravenously at three dose levels (0.9, 9 and 90 µg) to male Sprague Dawley rats weekly for 7 weeks, followed by a 14-day washout period. After sacrificing, the amount of gold was quantified in the liver, lungs, spleen, skeleton and carcass using neutron activation analysis (NAA). During the study, pre- and post (24 h) administration blood samples were collected from both the test and control groups, the latter which received an equal injection volume of normal saline. General health indicators were monitored together with markers of kidney and liver damage for acute and subchronic toxicity assessment. Histopathological assessments were done on the heart, kidneys, liver, lungs and spleen to assess any morphological changes as a result of the exposure to AuNPs. The mass measurements of all the groups showed a steady increase with no signs of overt toxicity. The liver had the highest amount of gold (µg) per gram of tissue after 56 days followed by the spleen, lungs, skeleton and carcass. Markers of kidney and liver damage showed similar trends between the pre and post samples within each group and across groups. The histopathological examination also showed no hepatotoxicity and nephrotoxicity. There was accumulation of Au in tissues after repeated dosing, albeit with no observable overt toxicity, kidney or liver damage. |
format | Online Article Text |
id | pubmed-6273121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62731212018-12-28 Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats Rambanapasi, Clinton Zeevaart, Jan Rijn Buntting, Hylton Bester, Cornelius Kotze, Deon Hayeshi, Rose Grobler, Anne Molecules Article Colloidal suspensions of 14 nm gold nanoparticles (AuNPs) were repeatedly administered intravenously at three dose levels (0.9, 9 and 90 µg) to male Sprague Dawley rats weekly for 7 weeks, followed by a 14-day washout period. After sacrificing, the amount of gold was quantified in the liver, lungs, spleen, skeleton and carcass using neutron activation analysis (NAA). During the study, pre- and post (24 h) administration blood samples were collected from both the test and control groups, the latter which received an equal injection volume of normal saline. General health indicators were monitored together with markers of kidney and liver damage for acute and subchronic toxicity assessment. Histopathological assessments were done on the heart, kidneys, liver, lungs and spleen to assess any morphological changes as a result of the exposure to AuNPs. The mass measurements of all the groups showed a steady increase with no signs of overt toxicity. The liver had the highest amount of gold (µg) per gram of tissue after 56 days followed by the spleen, lungs, skeleton and carcass. Markers of kidney and liver damage showed similar trends between the pre and post samples within each group and across groups. The histopathological examination also showed no hepatotoxicity and nephrotoxicity. There was accumulation of Au in tissues after repeated dosing, albeit with no observable overt toxicity, kidney or liver damage. MDPI 2016-06-10 /pmc/articles/PMC6273121/ /pubmed/27294904 http://dx.doi.org/10.3390/molecules21060763 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rambanapasi, Clinton Zeevaart, Jan Rijn Buntting, Hylton Bester, Cornelius Kotze, Deon Hayeshi, Rose Grobler, Anne Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats |
title | Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats |
title_full | Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats |
title_fullStr | Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats |
title_full_unstemmed | Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats |
title_short | Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats |
title_sort | bioaccumulation and subchronic toxicity of 14 nm gold nanoparticles in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273121/ https://www.ncbi.nlm.nih.gov/pubmed/27294904 http://dx.doi.org/10.3390/molecules21060763 |
work_keys_str_mv | AT rambanapasiclinton bioaccumulationandsubchronictoxicityof14nmgoldnanoparticlesinrats AT zeevaartjanrijn bioaccumulationandsubchronictoxicityof14nmgoldnanoparticlesinrats AT bunttinghylton bioaccumulationandsubchronictoxicityof14nmgoldnanoparticlesinrats AT bestercornelius bioaccumulationandsubchronictoxicityof14nmgoldnanoparticlesinrats AT kotzedeon bioaccumulationandsubchronictoxicityof14nmgoldnanoparticlesinrats AT hayeshirose bioaccumulationandsubchronictoxicityof14nmgoldnanoparticlesinrats AT grobleranne bioaccumulationandsubchronictoxicityof14nmgoldnanoparticlesinrats |