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Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats

Colloidal suspensions of 14 nm gold nanoparticles (AuNPs) were repeatedly administered intravenously at three dose levels (0.9, 9 and 90 µg) to male Sprague Dawley rats weekly for 7 weeks, followed by a 14-day washout period. After sacrificing, the amount of gold was quantified in the liver, lungs,...

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Autores principales: Rambanapasi, Clinton, Zeevaart, Jan Rijn, Buntting, Hylton, Bester, Cornelius, Kotze, Deon, Hayeshi, Rose, Grobler, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273121/
https://www.ncbi.nlm.nih.gov/pubmed/27294904
http://dx.doi.org/10.3390/molecules21060763
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author Rambanapasi, Clinton
Zeevaart, Jan Rijn
Buntting, Hylton
Bester, Cornelius
Kotze, Deon
Hayeshi, Rose
Grobler, Anne
author_facet Rambanapasi, Clinton
Zeevaart, Jan Rijn
Buntting, Hylton
Bester, Cornelius
Kotze, Deon
Hayeshi, Rose
Grobler, Anne
author_sort Rambanapasi, Clinton
collection PubMed
description Colloidal suspensions of 14 nm gold nanoparticles (AuNPs) were repeatedly administered intravenously at three dose levels (0.9, 9 and 90 µg) to male Sprague Dawley rats weekly for 7 weeks, followed by a 14-day washout period. After sacrificing, the amount of gold was quantified in the liver, lungs, spleen, skeleton and carcass using neutron activation analysis (NAA). During the study, pre- and post (24 h) administration blood samples were collected from both the test and control groups, the latter which received an equal injection volume of normal saline. General health indicators were monitored together with markers of kidney and liver damage for acute and subchronic toxicity assessment. Histopathological assessments were done on the heart, kidneys, liver, lungs and spleen to assess any morphological changes as a result of the exposure to AuNPs. The mass measurements of all the groups showed a steady increase with no signs of overt toxicity. The liver had the highest amount of gold (µg) per gram of tissue after 56 days followed by the spleen, lungs, skeleton and carcass. Markers of kidney and liver damage showed similar trends between the pre and post samples within each group and across groups. The histopathological examination also showed no hepatotoxicity and nephrotoxicity. There was accumulation of Au in tissues after repeated dosing, albeit with no observable overt toxicity, kidney or liver damage.
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spelling pubmed-62731212018-12-28 Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats Rambanapasi, Clinton Zeevaart, Jan Rijn Buntting, Hylton Bester, Cornelius Kotze, Deon Hayeshi, Rose Grobler, Anne Molecules Article Colloidal suspensions of 14 nm gold nanoparticles (AuNPs) were repeatedly administered intravenously at three dose levels (0.9, 9 and 90 µg) to male Sprague Dawley rats weekly for 7 weeks, followed by a 14-day washout period. After sacrificing, the amount of gold was quantified in the liver, lungs, spleen, skeleton and carcass using neutron activation analysis (NAA). During the study, pre- and post (24 h) administration blood samples were collected from both the test and control groups, the latter which received an equal injection volume of normal saline. General health indicators were monitored together with markers of kidney and liver damage for acute and subchronic toxicity assessment. Histopathological assessments were done on the heart, kidneys, liver, lungs and spleen to assess any morphological changes as a result of the exposure to AuNPs. The mass measurements of all the groups showed a steady increase with no signs of overt toxicity. The liver had the highest amount of gold (µg) per gram of tissue after 56 days followed by the spleen, lungs, skeleton and carcass. Markers of kidney and liver damage showed similar trends between the pre and post samples within each group and across groups. The histopathological examination also showed no hepatotoxicity and nephrotoxicity. There was accumulation of Au in tissues after repeated dosing, albeit with no observable overt toxicity, kidney or liver damage. MDPI 2016-06-10 /pmc/articles/PMC6273121/ /pubmed/27294904 http://dx.doi.org/10.3390/molecules21060763 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rambanapasi, Clinton
Zeevaart, Jan Rijn
Buntting, Hylton
Bester, Cornelius
Kotze, Deon
Hayeshi, Rose
Grobler, Anne
Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats
title Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats
title_full Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats
title_fullStr Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats
title_full_unstemmed Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats
title_short Bioaccumulation and Subchronic Toxicity of 14 nm Gold Nanoparticles in Rats
title_sort bioaccumulation and subchronic toxicity of 14 nm gold nanoparticles in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273121/
https://www.ncbi.nlm.nih.gov/pubmed/27294904
http://dx.doi.org/10.3390/molecules21060763
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