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A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization

A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the...

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Autores principales: Pinasseau, Lucie, Verbaere, Arnaud, Roques, Maryline, Meudec, Emmanuelle, Vallverdú-Queralt, Anna, Terrier, Nancy, Boulet, Jean-Claude, Cheynier, Véronique, Sommerer, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273201/
https://www.ncbi.nlm.nih.gov/pubmed/27775674
http://dx.doi.org/10.3390/molecules21101409
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author Pinasseau, Lucie
Verbaere, Arnaud
Roques, Maryline
Meudec, Emmanuelle
Vallverdú-Queralt, Anna
Terrier, Nancy
Boulet, Jean-Claude
Cheynier, Véronique
Sommerer, Nicolas
author_facet Pinasseau, Lucie
Verbaere, Arnaud
Roques, Maryline
Meudec, Emmanuelle
Vallverdú-Queralt, Anna
Terrier, Nancy
Boulet, Jean-Claude
Cheynier, Véronique
Sommerer, Nicolas
author_sort Pinasseau, Lucie
collection PubMed
description A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour. This method was applied to a set of 214 grape skin samples from 107 different red and white grape cultivars grown under two conditions in the vineyard, irrigated or non-irrigated. The results of triplicate analyses confirmed the robustness of the method, which was thus proven to be suitable for high-throughput and large-scale metabolomics studies. Moreover, these preliminary results suggest that analysis of tannin composition is relevant to investigate the genetic bases of grape response to drought.
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spelling pubmed-62732012018-12-28 A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization Pinasseau, Lucie Verbaere, Arnaud Roques, Maryline Meudec, Emmanuelle Vallverdú-Queralt, Anna Terrier, Nancy Boulet, Jean-Claude Cheynier, Véronique Sommerer, Nicolas Molecules Article A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour. This method was applied to a set of 214 grape skin samples from 107 different red and white grape cultivars grown under two conditions in the vineyard, irrigated or non-irrigated. The results of triplicate analyses confirmed the robustness of the method, which was thus proven to be suitable for high-throughput and large-scale metabolomics studies. Moreover, these preliminary results suggest that analysis of tannin composition is relevant to investigate the genetic bases of grape response to drought. MDPI 2016-10-21 /pmc/articles/PMC6273201/ /pubmed/27775674 http://dx.doi.org/10.3390/molecules21101409 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pinasseau, Lucie
Verbaere, Arnaud
Roques, Maryline
Meudec, Emmanuelle
Vallverdú-Queralt, Anna
Terrier, Nancy
Boulet, Jean-Claude
Cheynier, Véronique
Sommerer, Nicolas
A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization
title A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization
title_full A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization
title_fullStr A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization
title_full_unstemmed A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization
title_short A Fast and Robust UHPLC-MRM-MS Method to Characterize and Quantify Grape Skin Tannins after Chemical Depolymerization
title_sort fast and robust uhplc-mrm-ms method to characterize and quantify grape skin tannins after chemical depolymerization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273201/
https://www.ncbi.nlm.nih.gov/pubmed/27775674
http://dx.doi.org/10.3390/molecules21101409
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