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Synthesis and Structural Investigation of New Bio-Relevant Complexes of Lanthanides with 5-Hydroxyflavone: DNA Binding and Protein Interaction Studies

In the present work, we attempted to develop new metal coordination complexes of the natural flavonoid 5-hydroxyflavone with Sm(III), Eu(III), Gd(III), Tb(III). The resultant hydroxo complexes have been characterized by a variety of spectroscopic techniques, including fluorescence, FT-IR, UV-Vis, EP...

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Autores principales: Munteanu, Alexandra-Cristina, Badea, Mihaela, Olar, Rodica, Silvestro, Luigi, Dulea, Constanţa, Negut, Constantin-Daniel, Uivarosi, Valentina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273368/
https://www.ncbi.nlm.nih.gov/pubmed/27999283
http://dx.doi.org/10.3390/molecules21121737
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author Munteanu, Alexandra-Cristina
Badea, Mihaela
Olar, Rodica
Silvestro, Luigi
Dulea, Constanţa
Negut, Constantin-Daniel
Uivarosi, Valentina
author_facet Munteanu, Alexandra-Cristina
Badea, Mihaela
Olar, Rodica
Silvestro, Luigi
Dulea, Constanţa
Negut, Constantin-Daniel
Uivarosi, Valentina
author_sort Munteanu, Alexandra-Cristina
collection PubMed
description In the present work, we attempted to develop new metal coordination complexes of the natural flavonoid 5-hydroxyflavone with Sm(III), Eu(III), Gd(III), Tb(III). The resultant hydroxo complexes have been characterized by a variety of spectroscopic techniques, including fluorescence, FT-IR, UV-Vis, EPR and mass spectral studies. The general chemical formula of the complexes is [Ln(C(15)H(9)O(3))(3)(OH)(2)(H(2)O)(x)]·nH(2)O, where Ln is the lanthanide cation and x = 0 for Sm(III), x = 1 for Eu(III), Gd(III), Tb(III) and n = 0 for Sm(III), Gd(III), Tb(III), n = 1 for Eu(III), respectively. The proposed structures of the complexes were optimized by DFT calculations. Theoretical calculations and experimental determinations sustain the proposed structures of the hydroxo complexes, with two molecules of 5-hydroxyflavone acting as monoanionic bidentate chelate ligands. The interaction of the complexes with calf thymus DNA has been explored by fluorescence titration and UV-Vis absorption binding studies, and revealed that the synthesized complexes interact with DNA with binding constants (K(b)) ~ 10(4). Human serum albumin (HSA) and transferrin (Tf) binding studies have also been performed by fluorescence titration techniques (fluorescence quenching studies, synchronous fluorescence spectra). The apparent association constants (K(a)) and thermodynamic parameters have been calculated from the fluorescence quenching experiment at 299 K, 308 K, and 318 K. The quenching curves indicate that the complexes bind to HSA with smaller affinity than the ligand, but to Tf with higher binding affinities than the ligand.
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spelling pubmed-62733682018-12-28 Synthesis and Structural Investigation of New Bio-Relevant Complexes of Lanthanides with 5-Hydroxyflavone: DNA Binding and Protein Interaction Studies Munteanu, Alexandra-Cristina Badea, Mihaela Olar, Rodica Silvestro, Luigi Dulea, Constanţa Negut, Constantin-Daniel Uivarosi, Valentina Molecules Article In the present work, we attempted to develop new metal coordination complexes of the natural flavonoid 5-hydroxyflavone with Sm(III), Eu(III), Gd(III), Tb(III). The resultant hydroxo complexes have been characterized by a variety of spectroscopic techniques, including fluorescence, FT-IR, UV-Vis, EPR and mass spectral studies. The general chemical formula of the complexes is [Ln(C(15)H(9)O(3))(3)(OH)(2)(H(2)O)(x)]·nH(2)O, where Ln is the lanthanide cation and x = 0 for Sm(III), x = 1 for Eu(III), Gd(III), Tb(III) and n = 0 for Sm(III), Gd(III), Tb(III), n = 1 for Eu(III), respectively. The proposed structures of the complexes were optimized by DFT calculations. Theoretical calculations and experimental determinations sustain the proposed structures of the hydroxo complexes, with two molecules of 5-hydroxyflavone acting as monoanionic bidentate chelate ligands. The interaction of the complexes with calf thymus DNA has been explored by fluorescence titration and UV-Vis absorption binding studies, and revealed that the synthesized complexes interact with DNA with binding constants (K(b)) ~ 10(4). Human serum albumin (HSA) and transferrin (Tf) binding studies have also been performed by fluorescence titration techniques (fluorescence quenching studies, synchronous fluorescence spectra). The apparent association constants (K(a)) and thermodynamic parameters have been calculated from the fluorescence quenching experiment at 299 K, 308 K, and 318 K. The quenching curves indicate that the complexes bind to HSA with smaller affinity than the ligand, but to Tf with higher binding affinities than the ligand. MDPI 2016-12-16 /pmc/articles/PMC6273368/ /pubmed/27999283 http://dx.doi.org/10.3390/molecules21121737 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Munteanu, Alexandra-Cristina
Badea, Mihaela
Olar, Rodica
Silvestro, Luigi
Dulea, Constanţa
Negut, Constantin-Daniel
Uivarosi, Valentina
Synthesis and Structural Investigation of New Bio-Relevant Complexes of Lanthanides with 5-Hydroxyflavone: DNA Binding and Protein Interaction Studies
title Synthesis and Structural Investigation of New Bio-Relevant Complexes of Lanthanides with 5-Hydroxyflavone: DNA Binding and Protein Interaction Studies
title_full Synthesis and Structural Investigation of New Bio-Relevant Complexes of Lanthanides with 5-Hydroxyflavone: DNA Binding and Protein Interaction Studies
title_fullStr Synthesis and Structural Investigation of New Bio-Relevant Complexes of Lanthanides with 5-Hydroxyflavone: DNA Binding and Protein Interaction Studies
title_full_unstemmed Synthesis and Structural Investigation of New Bio-Relevant Complexes of Lanthanides with 5-Hydroxyflavone: DNA Binding and Protein Interaction Studies
title_short Synthesis and Structural Investigation of New Bio-Relevant Complexes of Lanthanides with 5-Hydroxyflavone: DNA Binding and Protein Interaction Studies
title_sort synthesis and structural investigation of new bio-relevant complexes of lanthanides with 5-hydroxyflavone: dna binding and protein interaction studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273368/
https://www.ncbi.nlm.nih.gov/pubmed/27999283
http://dx.doi.org/10.3390/molecules21121737
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