Cargando…
Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways
Periodontitis is a chronic inflammatory disease that leads to destruction of tooth supporting tissues. Porphyromonas gingivalis (P. gingivalis), especially its lipopolysaccharides (LPS), is one of major pathogens that cause periodontitis. Bee venom (BV) has been widely used as a traditional medicine...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273372/ https://www.ncbi.nlm.nih.gov/pubmed/27834922 http://dx.doi.org/10.3390/molecules21111508 |
_version_ | 1783377369275301888 |
---|---|
author | Kim, Woon-Hae An, Hyun-Jin Kim, Jung-Yeon Gwon, Mi-Gyeong Gu, Hyemin Park, Jae-Bok Sung, Woo Jung Kwon, Yong-Chul Park, Kyung-Duck Han, Sang Mi Park, Kwan-Kyu |
author_facet | Kim, Woon-Hae An, Hyun-Jin Kim, Jung-Yeon Gwon, Mi-Gyeong Gu, Hyemin Park, Jae-Bok Sung, Woo Jung Kwon, Yong-Chul Park, Kyung-Duck Han, Sang Mi Park, Kwan-Kyu |
author_sort | Kim, Woon-Hae |
collection | PubMed |
description | Periodontitis is a chronic inflammatory disease that leads to destruction of tooth supporting tissues. Porphyromonas gingivalis (P. gingivalis), especially its lipopolysaccharides (LPS), is one of major pathogens that cause periodontitis. Bee venom (BV) has been widely used as a traditional medicine for various diseases. Previous studies have demonstrated the anti-inflammatory, anti-bacterial effects of BV. However, a direct role and cellular mechanism of BV on periodontitis-like human keratinocytes have not been explored. Therefore, we investigated the anti-inflammatory mechanism of BV against P. gingivalis LPS (PgLPS)-induced HaCaT human keratinocyte cell line. The anti-inflammatory effect of BV was demonstrated by various molecular biological methods. The results showed that PgLPS increased the expression of Toll-like receptor (TLR)-4 and pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, and interferon (IFN)-γ. In addition, PgLPS induced activation of the signaling pathways of inflammatory cytokines-related transcription factors, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein 1 (AP-1). BV effectively inhibited those pro-inflammatory cytokines through suppression of NF-κB and AP-1 signaling pathways. These results suggest that administration of BV attenuates PgLPS-induced inflammatory responses. Furthermore, BV may be a useful treatment to anti-inflammatory therapy for periodontitis. |
format | Online Article Text |
id | pubmed-6273372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62733722018-12-28 Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways Kim, Woon-Hae An, Hyun-Jin Kim, Jung-Yeon Gwon, Mi-Gyeong Gu, Hyemin Park, Jae-Bok Sung, Woo Jung Kwon, Yong-Chul Park, Kyung-Duck Han, Sang Mi Park, Kwan-Kyu Molecules Article Periodontitis is a chronic inflammatory disease that leads to destruction of tooth supporting tissues. Porphyromonas gingivalis (P. gingivalis), especially its lipopolysaccharides (LPS), is one of major pathogens that cause periodontitis. Bee venom (BV) has been widely used as a traditional medicine for various diseases. Previous studies have demonstrated the anti-inflammatory, anti-bacterial effects of BV. However, a direct role and cellular mechanism of BV on periodontitis-like human keratinocytes have not been explored. Therefore, we investigated the anti-inflammatory mechanism of BV against P. gingivalis LPS (PgLPS)-induced HaCaT human keratinocyte cell line. The anti-inflammatory effect of BV was demonstrated by various molecular biological methods. The results showed that PgLPS increased the expression of Toll-like receptor (TLR)-4 and pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, and interferon (IFN)-γ. In addition, PgLPS induced activation of the signaling pathways of inflammatory cytokines-related transcription factors, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein 1 (AP-1). BV effectively inhibited those pro-inflammatory cytokines through suppression of NF-κB and AP-1 signaling pathways. These results suggest that administration of BV attenuates PgLPS-induced inflammatory responses. Furthermore, BV may be a useful treatment to anti-inflammatory therapy for periodontitis. MDPI 2016-11-10 /pmc/articles/PMC6273372/ /pubmed/27834922 http://dx.doi.org/10.3390/molecules21111508 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Woon-Hae An, Hyun-Jin Kim, Jung-Yeon Gwon, Mi-Gyeong Gu, Hyemin Park, Jae-Bok Sung, Woo Jung Kwon, Yong-Chul Park, Kyung-Duck Han, Sang Mi Park, Kwan-Kyu Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways |
title | Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways |
title_full | Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways |
title_fullStr | Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways |
title_full_unstemmed | Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways |
title_short | Bee Venom Inhibits Porphyromonas gingivalis Lipopolysaccharides-Induced Pro-Inflammatory Cytokines through Suppression of NF-κB and AP-1 Signaling Pathways |
title_sort | bee venom inhibits porphyromonas gingivalis lipopolysaccharides-induced pro-inflammatory cytokines through suppression of nf-κb and ap-1 signaling pathways |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273372/ https://www.ncbi.nlm.nih.gov/pubmed/27834922 http://dx.doi.org/10.3390/molecules21111508 |
work_keys_str_mv | AT kimwoonhae beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT anhyunjin beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT kimjungyeon beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT gwonmigyeong beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT guhyemin beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT parkjaebok beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT sungwoojung beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT kwonyongchul beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT parkkyungduck beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT hansangmi beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways AT parkkwankyu beevenominhibitsporphyromonasgingivalislipopolysaccharidesinducedproinflammatorycytokinesthroughsuppressionofnfkbandap1signalingpathways |