Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy

The species Deguelia utilis and Deguelia rufescens var. urucu, popularly known as “timbó,” have been used for many years as rotenone sources in insecticide formulations. In this work, a method was developed and validated using a high-performance liquid chromatography-photodiode array (HPLC-PDA) syst...

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Autores principales: da Costa, Danielle, e Silva, Consuelo, Pinheiro, Aline, Frommenwiler, Débora, Arruda, Mara, Guilhon, Giselle, Alves, Cláudio, Arruda, Alberto, Da Silva, Milton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273376/
https://www.ncbi.nlm.nih.gov/pubmed/27144548
http://dx.doi.org/10.3390/molecules21050569
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author da Costa, Danielle
e Silva, Consuelo
Pinheiro, Aline
Frommenwiler, Débora
Arruda, Mara
Guilhon, Giselle
Alves, Cláudio
Arruda, Alberto
Da Silva, Milton
author_facet da Costa, Danielle
e Silva, Consuelo
Pinheiro, Aline
Frommenwiler, Débora
Arruda, Mara
Guilhon, Giselle
Alves, Cláudio
Arruda, Alberto
Da Silva, Milton
author_sort da Costa, Danielle
collection PubMed
description The species Deguelia utilis and Deguelia rufescens var. urucu, popularly known as “timbó,” have been used for many years as rotenone sources in insecticide formulations. In this work, a method was developed and validated using a high-performance liquid chromatography-photodiode array (HPLC-PDA) system, and results were analyzed using hierarchical cluster analysis (HCA). By quantifying the major rotenoids of these species, it was possible to establish a linear relation between them. The ratio between the concentrations of rotenone and deguelin for D. utilis is approximately 1:0.8, respectively, while for D. rufescens var. urucu it is 2:1. These results may help to distinguish these species contributing to their taxonomic identification.
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spelling pubmed-62733762018-12-28 Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy da Costa, Danielle e Silva, Consuelo Pinheiro, Aline Frommenwiler, Débora Arruda, Mara Guilhon, Giselle Alves, Cláudio Arruda, Alberto Da Silva, Milton Molecules Article The species Deguelia utilis and Deguelia rufescens var. urucu, popularly known as “timbó,” have been used for many years as rotenone sources in insecticide formulations. In this work, a method was developed and validated using a high-performance liquid chromatography-photodiode array (HPLC-PDA) system, and results were analyzed using hierarchical cluster analysis (HCA). By quantifying the major rotenoids of these species, it was possible to establish a linear relation between them. The ratio between the concentrations of rotenone and deguelin for D. utilis is approximately 1:0.8, respectively, while for D. rufescens var. urucu it is 2:1. These results may help to distinguish these species contributing to their taxonomic identification. MDPI 2016-04-30 /pmc/articles/PMC6273376/ /pubmed/27144548 http://dx.doi.org/10.3390/molecules21050569 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Costa, Danielle
e Silva, Consuelo
Pinheiro, Aline
Frommenwiler, Débora
Arruda, Mara
Guilhon, Giselle
Alves, Cláudio
Arruda, Alberto
Da Silva, Milton
Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy
title Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy
title_full Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy
title_fullStr Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy
title_full_unstemmed Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy
title_short Using LC and Hierarchical Cluster Analysis as Tools to Distinguish Timbó Collections into Two Deguelia Species: A Contribution to Chemotaxonomy
title_sort using lc and hierarchical cluster analysis as tools to distinguish timbó collections into two deguelia species: a contribution to chemotaxonomy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273376/
https://www.ncbi.nlm.nih.gov/pubmed/27144548
http://dx.doi.org/10.3390/molecules21050569
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