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Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse

3-Mercaptopyruvate sulfurtransferase (MST) is one of the principal enzymes for the production of hydrogen sulfide and polysulfides in mammalians, and emerging evidence supports the physiological significance of MST. As a fundamental study of the physiology and pathobiology of MST, it is necessary to...

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Autores principales: Tomita, Masahiro, Nagahara, Noriyuki, Ito, Takaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273466/
https://www.ncbi.nlm.nih.gov/pubmed/27973427
http://dx.doi.org/10.3390/molecules21121707
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author Tomita, Masahiro
Nagahara, Noriyuki
Ito, Takaaki
author_facet Tomita, Masahiro
Nagahara, Noriyuki
Ito, Takaaki
author_sort Tomita, Masahiro
collection PubMed
description 3-Mercaptopyruvate sulfurtransferase (MST) is one of the principal enzymes for the production of hydrogen sulfide and polysulfides in mammalians, and emerging evidence supports the physiological significance of MST. As a fundamental study of the physiology and pathobiology of MST, it is necessary to establish the tissue distribution of MST in mice. In the present study, the expression of MST in various organs of adult and fetal mice was analyzed by Western blotting and enzyme-immunohistochemistry. Moreover, the histology of MST gene–deficient mice was examined. Western blotting revealed that all organs examined had MST. The brain, liver, kidneys testes, and endocrine organs contained large amounts of MST, but the lungs, spleen, thymus, and small intestine did not. Immunohistochemically, the MST expression pattern varies in a cell-specific manner. In the brain, neural and glial cells are positively stained; in the lung, bronchiolar cells are preferentially stained; in the liver, hepatocytes around central veins are more strongly stained; renal convoluted cells are strongly stained; and pancreatic islets are strongly stained. Fetal tissues were studied, and MST expression was found to be similar before and after birth. Histological observation revealed no remarkable findings in MST gene–deficient mice. The present study revealed fundamental information regarding the MST expression of various organs in adult and fetal mice, and the morphological phenotype of MST gene–deficient mice.
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spelling pubmed-62734662018-12-28 Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse Tomita, Masahiro Nagahara, Noriyuki Ito, Takaaki Molecules Article 3-Mercaptopyruvate sulfurtransferase (MST) is one of the principal enzymes for the production of hydrogen sulfide and polysulfides in mammalians, and emerging evidence supports the physiological significance of MST. As a fundamental study of the physiology and pathobiology of MST, it is necessary to establish the tissue distribution of MST in mice. In the present study, the expression of MST in various organs of adult and fetal mice was analyzed by Western blotting and enzyme-immunohistochemistry. Moreover, the histology of MST gene–deficient mice was examined. Western blotting revealed that all organs examined had MST. The brain, liver, kidneys testes, and endocrine organs contained large amounts of MST, but the lungs, spleen, thymus, and small intestine did not. Immunohistochemically, the MST expression pattern varies in a cell-specific manner. In the brain, neural and glial cells are positively stained; in the lung, bronchiolar cells are preferentially stained; in the liver, hepatocytes around central veins are more strongly stained; renal convoluted cells are strongly stained; and pancreatic islets are strongly stained. Fetal tissues were studied, and MST expression was found to be similar before and after birth. Histological observation revealed no remarkable findings in MST gene–deficient mice. The present study revealed fundamental information regarding the MST expression of various organs in adult and fetal mice, and the morphological phenotype of MST gene–deficient mice. MDPI 2016-12-11 /pmc/articles/PMC6273466/ /pubmed/27973427 http://dx.doi.org/10.3390/molecules21121707 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tomita, Masahiro
Nagahara, Noriyuki
Ito, Takaaki
Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse
title Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse
title_full Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse
title_fullStr Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse
title_full_unstemmed Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse
title_short Expression of 3-Mercaptopyruvate Sulfurtransferase in the Mouse
title_sort expression of 3-mercaptopyruvate sulfurtransferase in the mouse
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273466/
https://www.ncbi.nlm.nih.gov/pubmed/27973427
http://dx.doi.org/10.3390/molecules21121707
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