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Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ

Background: Spermatozoa become mature and acquire fertilizing capacity during their passage through the epididymal lumen. In this study, we identified new epididymal luminal fluid proteins involved in sperm maturation in infertile rats by dutasteride, a dual 5α-reductase inhibitor, in order to provi...

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Autores principales: Xie, Shu-Wu, Li, Guo-Ting, Qu, Li-Juan, Cao, Yang, Wang, Qian, Zhou, Jie-Yun, Zhong, Rui-Hua, Guo, Xiang-Jie, Zhu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273551/
https://www.ncbi.nlm.nih.gov/pubmed/27187330
http://dx.doi.org/10.3390/molecules21050602
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author Xie, Shu-Wu
Li, Guo-Ting
Qu, Li-Juan
Cao, Yang
Wang, Qian
Zhou, Jie-Yun
Zhong, Rui-Hua
Guo, Xiang-Jie
Zhu, Yan
author_facet Xie, Shu-Wu
Li, Guo-Ting
Qu, Li-Juan
Cao, Yang
Wang, Qian
Zhou, Jie-Yun
Zhong, Rui-Hua
Guo, Xiang-Jie
Zhu, Yan
author_sort Xie, Shu-Wu
collection PubMed
description Background: Spermatozoa become mature and acquire fertilizing capacity during their passage through the epididymal lumen. In this study, we identified new epididymal luminal fluid proteins involved in sperm maturation in infertile rats by dutasteride, a dual 5α-reductase inhibitor, in order to provide potential epididymal targets for new contraceptives and infertility treatment. Methods: Male rats were treated with dutasteride for 28 consecutive days. We observed the protein expression profiles in the epididymal luminal fluids in infertile and normal rats using isobaric tags for relative and absolute quantitation (iTRAQ) technique. The confidence of proteome data was validated by enzyme-linked immunosorbent assays. Results: 1045 proteins were tested, and 23 of them presented different expression profiling in the infertile and normal rats. The seven proteins were down-regulated, and 16 proteins were up-regulated. Among the seven proteins which were significantly down-regulated by dutasteride in the epididymal luminal fluids, there were three β-defensins (Defb2, Defb18 and Defb39), which maybe the key proteins involved in epididymal sperm maturation and male fertility. Conclusions: We report for the first time that dutasteride influences the protein expression profiling in the epididymal luminal fluids of rats, and this result provides some new epididymal targets for male contraception and infertility therapy.
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spelling pubmed-62735512018-12-28 Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ Xie, Shu-Wu Li, Guo-Ting Qu, Li-Juan Cao, Yang Wang, Qian Zhou, Jie-Yun Zhong, Rui-Hua Guo, Xiang-Jie Zhu, Yan Molecules Article Background: Spermatozoa become mature and acquire fertilizing capacity during their passage through the epididymal lumen. In this study, we identified new epididymal luminal fluid proteins involved in sperm maturation in infertile rats by dutasteride, a dual 5α-reductase inhibitor, in order to provide potential epididymal targets for new contraceptives and infertility treatment. Methods: Male rats were treated with dutasteride for 28 consecutive days. We observed the protein expression profiles in the epididymal luminal fluids in infertile and normal rats using isobaric tags for relative and absolute quantitation (iTRAQ) technique. The confidence of proteome data was validated by enzyme-linked immunosorbent assays. Results: 1045 proteins were tested, and 23 of them presented different expression profiling in the infertile and normal rats. The seven proteins were down-regulated, and 16 proteins were up-regulated. Among the seven proteins which were significantly down-regulated by dutasteride in the epididymal luminal fluids, there were three β-defensins (Defb2, Defb18 and Defb39), which maybe the key proteins involved in epididymal sperm maturation and male fertility. Conclusions: We report for the first time that dutasteride influences the protein expression profiling in the epididymal luminal fluids of rats, and this result provides some new epididymal targets for male contraception and infertility therapy. MDPI 2016-05-11 /pmc/articles/PMC6273551/ /pubmed/27187330 http://dx.doi.org/10.3390/molecules21050602 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xie, Shu-Wu
Li, Guo-Ting
Qu, Li-Juan
Cao, Yang
Wang, Qian
Zhou, Jie-Yun
Zhong, Rui-Hua
Guo, Xiang-Jie
Zhu, Yan
Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ
title Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ
title_full Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ
title_fullStr Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ
title_full_unstemmed Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ
title_short Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ
title_sort identification of new epididymal luminal fluid proteins involved in sperm maturation in infertile rats treated by dutasteride using itraq
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273551/
https://www.ncbi.nlm.nih.gov/pubmed/27187330
http://dx.doi.org/10.3390/molecules21050602
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