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Trans-Fatty Acids Aggravate Obesity, Insulin Resistance and Hepatic Steatosis in C57BL/6 Mice, Possibly by Suppressing the IRS1 Dependent Pathway

Trans-fatty acid consumption has been reported as a risk factor for metabolic disorders and targeted organ damages. Nonetheless, little is known about the roles and mechanisms of trans-fatty acids in obesity, insulin resistance (IR) and hepatic steatosis. Adult C57BL/6 male mice were fed with four d...

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Autores principales: Zhao, Xiaona, Shen, Cheng, Zhu, Hong, Wang, Cong, Liu, Xiangwei, Sun, Xiaolei, Han, Shasha, Wang, Peng, Dong, Zhen, Ma, Xin, Hu, Kai, Sun, Aijun, Ge, Junbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273562/
https://www.ncbi.nlm.nih.gov/pubmed/27248994
http://dx.doi.org/10.3390/molecules21060705
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author Zhao, Xiaona
Shen, Cheng
Zhu, Hong
Wang, Cong
Liu, Xiangwei
Sun, Xiaolei
Han, Shasha
Wang, Peng
Dong, Zhen
Ma, Xin
Hu, Kai
Sun, Aijun
Ge, Junbo
author_facet Zhao, Xiaona
Shen, Cheng
Zhu, Hong
Wang, Cong
Liu, Xiangwei
Sun, Xiaolei
Han, Shasha
Wang, Peng
Dong, Zhen
Ma, Xin
Hu, Kai
Sun, Aijun
Ge, Junbo
author_sort Zhao, Xiaona
collection PubMed
description Trans-fatty acid consumption has been reported as a risk factor for metabolic disorders and targeted organ damages. Nonetheless, little is known about the roles and mechanisms of trans-fatty acids in obesity, insulin resistance (IR) and hepatic steatosis. Adult C57BL/6 male mice were fed with four different diets for 20 weeks: normal diet (ND), high fat diet (HFD), low trans-fatty acids diet (LTD) and high trans-fatty acid diet (HTD). The diet-induced metabolic disorders were assessed by evaluating body weight, glucose tolerance test, hepatic steatosis and plasma lipid profiles post 20-week diet. Histological (H&E, Oil-Red-O) staining and western blot analysis were employed to assess liver steatosis and potential signaling pathways. After 20-weeks of diet, the body weights of the four groups were 29.61 ± 1.89 g (ND), 39.04 ± 4.27 g (HFD), 34.09 ± 2.62 g (LTD) and 43.78 ± 4.27 g (HTD) (p < 0.05), respectively. HFD intake significantly impaired glucose tolerance, which was impaired further in the mice consuming the HTD diet. The effect was further exacerbated by HTD diet. Moreover, the HTD group exhibited significantly more severe liver steatosis compared with HFD group possibly through regulating adipose triglyceride lipase. The group consuming the HTD also exhibited significantly reduced levels of IRS1, phosphor-PKC and phosphor-AKT. These results support our hypothesis that consumption of a diet high in trans-fatty acids induces higher rates of obesity, IR and hepatic steatosis in male C57BL/6 mice, possibly by suppressing the IRS1dependent pathway.
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spelling pubmed-62735622018-12-28 Trans-Fatty Acids Aggravate Obesity, Insulin Resistance and Hepatic Steatosis in C57BL/6 Mice, Possibly by Suppressing the IRS1 Dependent Pathway Zhao, Xiaona Shen, Cheng Zhu, Hong Wang, Cong Liu, Xiangwei Sun, Xiaolei Han, Shasha Wang, Peng Dong, Zhen Ma, Xin Hu, Kai Sun, Aijun Ge, Junbo Molecules Article Trans-fatty acid consumption has been reported as a risk factor for metabolic disorders and targeted organ damages. Nonetheless, little is known about the roles and mechanisms of trans-fatty acids in obesity, insulin resistance (IR) and hepatic steatosis. Adult C57BL/6 male mice were fed with four different diets for 20 weeks: normal diet (ND), high fat diet (HFD), low trans-fatty acids diet (LTD) and high trans-fatty acid diet (HTD). The diet-induced metabolic disorders were assessed by evaluating body weight, glucose tolerance test, hepatic steatosis and plasma lipid profiles post 20-week diet. Histological (H&E, Oil-Red-O) staining and western blot analysis were employed to assess liver steatosis and potential signaling pathways. After 20-weeks of diet, the body weights of the four groups were 29.61 ± 1.89 g (ND), 39.04 ± 4.27 g (HFD), 34.09 ± 2.62 g (LTD) and 43.78 ± 4.27 g (HTD) (p < 0.05), respectively. HFD intake significantly impaired glucose tolerance, which was impaired further in the mice consuming the HTD diet. The effect was further exacerbated by HTD diet. Moreover, the HTD group exhibited significantly more severe liver steatosis compared with HFD group possibly through regulating adipose triglyceride lipase. The group consuming the HTD also exhibited significantly reduced levels of IRS1, phosphor-PKC and phosphor-AKT. These results support our hypothesis that consumption of a diet high in trans-fatty acids induces higher rates of obesity, IR and hepatic steatosis in male C57BL/6 mice, possibly by suppressing the IRS1dependent pathway. MDPI 2016-05-30 /pmc/articles/PMC6273562/ /pubmed/27248994 http://dx.doi.org/10.3390/molecules21060705 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Xiaona
Shen, Cheng
Zhu, Hong
Wang, Cong
Liu, Xiangwei
Sun, Xiaolei
Han, Shasha
Wang, Peng
Dong, Zhen
Ma, Xin
Hu, Kai
Sun, Aijun
Ge, Junbo
Trans-Fatty Acids Aggravate Obesity, Insulin Resistance and Hepatic Steatosis in C57BL/6 Mice, Possibly by Suppressing the IRS1 Dependent Pathway
title Trans-Fatty Acids Aggravate Obesity, Insulin Resistance and Hepatic Steatosis in C57BL/6 Mice, Possibly by Suppressing the IRS1 Dependent Pathway
title_full Trans-Fatty Acids Aggravate Obesity, Insulin Resistance and Hepatic Steatosis in C57BL/6 Mice, Possibly by Suppressing the IRS1 Dependent Pathway
title_fullStr Trans-Fatty Acids Aggravate Obesity, Insulin Resistance and Hepatic Steatosis in C57BL/6 Mice, Possibly by Suppressing the IRS1 Dependent Pathway
title_full_unstemmed Trans-Fatty Acids Aggravate Obesity, Insulin Resistance and Hepatic Steatosis in C57BL/6 Mice, Possibly by Suppressing the IRS1 Dependent Pathway
title_short Trans-Fatty Acids Aggravate Obesity, Insulin Resistance and Hepatic Steatosis in C57BL/6 Mice, Possibly by Suppressing the IRS1 Dependent Pathway
title_sort trans-fatty acids aggravate obesity, insulin resistance and hepatic steatosis in c57bl/6 mice, possibly by suppressing the irs1 dependent pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273562/
https://www.ncbi.nlm.nih.gov/pubmed/27248994
http://dx.doi.org/10.3390/molecules21060705
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