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Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter

Measuring the antioxidant capacity of foods is essential, as a means of quality control to ensure that the final product reaching the consumer will be of high standards. Despite the already existing assays with which the antioxidant activity is estimated, new, faster and low cost methods are always...

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Autores principales: Priftis, Alexandros, Stagos, Dimitrios, Tzioumakis, Nikolaos, Konstantinopoulos, Konstantinos, Patouna, Anastasia, Papadopoulos, Georgios E., Tsatsakis, Aristides, Kouretas, Dimitrios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273567/
https://www.ncbi.nlm.nih.gov/pubmed/27589706
http://dx.doi.org/10.3390/molecules21091154
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author Priftis, Alexandros
Stagos, Dimitrios
Tzioumakis, Nikolaos
Konstantinopoulos, Konstantinos
Patouna, Anastasia
Papadopoulos, Georgios E.
Tsatsakis, Aristides
Kouretas, Dimitrios
author_facet Priftis, Alexandros
Stagos, Dimitrios
Tzioumakis, Nikolaos
Konstantinopoulos, Konstantinos
Patouna, Anastasia
Papadopoulos, Georgios E.
Tsatsakis, Aristides
Kouretas, Dimitrios
author_sort Priftis, Alexandros
collection PubMed
description Measuring the antioxidant capacity of foods is essential, as a means of quality control to ensure that the final product reaching the consumer will be of high standards. Despite the already existing assays with which the antioxidant activity is estimated, new, faster and low cost methods are always sought. Therefore, we have developed a novel colorimeter and combined it with a slightly modified DPPH assay, thus creating a kit that can assess the antioxidant capacity of liquids (e.g., different types of coffee, beer, wine, juices) in a quite fast and low cost manner. The accuracy of the colorimeter was ensured by comparing it to a fully validated Hitachi U-1900 spectrophotometer, and a coefficient was calculated to eliminate the observed differences. In addition, a new, user friendly software was developed, in order to render the procedure as easy as possible, while allowing a central monitoring of the obtained results. Overall, a novel kit was developed, with which the antioxidant activity of liquids can be measured, firstly to ensure their quality and secondly to assess the amount of antioxidants consumed with the respective food.
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spelling pubmed-62735672018-12-28 Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter Priftis, Alexandros Stagos, Dimitrios Tzioumakis, Nikolaos Konstantinopoulos, Konstantinos Patouna, Anastasia Papadopoulos, Georgios E. Tsatsakis, Aristides Kouretas, Dimitrios Molecules Article Measuring the antioxidant capacity of foods is essential, as a means of quality control to ensure that the final product reaching the consumer will be of high standards. Despite the already existing assays with which the antioxidant activity is estimated, new, faster and low cost methods are always sought. Therefore, we have developed a novel colorimeter and combined it with a slightly modified DPPH assay, thus creating a kit that can assess the antioxidant capacity of liquids (e.g., different types of coffee, beer, wine, juices) in a quite fast and low cost manner. The accuracy of the colorimeter was ensured by comparing it to a fully validated Hitachi U-1900 spectrophotometer, and a coefficient was calculated to eliminate the observed differences. In addition, a new, user friendly software was developed, in order to render the procedure as easy as possible, while allowing a central monitoring of the obtained results. Overall, a novel kit was developed, with which the antioxidant activity of liquids can be measured, firstly to ensure their quality and secondly to assess the amount of antioxidants consumed with the respective food. MDPI 2016-08-30 /pmc/articles/PMC6273567/ /pubmed/27589706 http://dx.doi.org/10.3390/molecules21091154 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Priftis, Alexandros
Stagos, Dimitrios
Tzioumakis, Nikolaos
Konstantinopoulos, Konstantinos
Patouna, Anastasia
Papadopoulos, Georgios E.
Tsatsakis, Aristides
Kouretas, Dimitrios
Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter
title Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter
title_full Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter
title_fullStr Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter
title_full_unstemmed Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter
title_short Development and Validation of a Kit to Measure Drink Antioxidant Capacity Using a Novel Colorimeter
title_sort development and validation of a kit to measure drink antioxidant capacity using a novel colorimeter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273567/
https://www.ncbi.nlm.nih.gov/pubmed/27589706
http://dx.doi.org/10.3390/molecules21091154
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