The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units

We report the draft genome sequence of Actinokineospora bangkokensis 44EHW(T), the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clus...

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Autores principales: Greule, Anja, Intra, Bungonsiri, Flemming, Stephan, Rommel, Marcel G. E., Panbangred, Watanalai, Bechthold, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273641/
https://www.ncbi.nlm.nih.gov/pubmed/27886115
http://dx.doi.org/10.3390/molecules21111607
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author Greule, Anja
Intra, Bungonsiri
Flemming, Stephan
Rommel, Marcel G. E.
Panbangred, Watanalai
Bechthold, Andreas
author_facet Greule, Anja
Intra, Bungonsiri
Flemming, Stephan
Rommel, Marcel G. E.
Panbangred, Watanalai
Bechthold, Andreas
author_sort Greule, Anja
collection PubMed
description We report the draft genome sequence of Actinokineospora bangkokensis 44EHW(T), the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain.
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spelling pubmed-62736412018-12-28 The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units Greule, Anja Intra, Bungonsiri Flemming, Stephan Rommel, Marcel G. E. Panbangred, Watanalai Bechthold, Andreas Molecules Article We report the draft genome sequence of Actinokineospora bangkokensis 44EHW(T), the producer of the antifungal polyene compounds, thailandins A and B. The sequence contains 7.45 Mb, 74.1% GC content and 35 putative gene clusters for the biosynthesis of secondary metabolites. There are three gene clusters encoding large polyketide synthases of type I. Annotation of the ORF functions and targeted gene disruption enabled us to identify the cluster for thailandin biosynthesis. We propose a plausible biosynthetic pathway for thailandin, where the unusual butylmalonyl-CoA extender unit is incorporated and results in an untypical side chain. MDPI 2016-11-23 /pmc/articles/PMC6273641/ /pubmed/27886115 http://dx.doi.org/10.3390/molecules21111607 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Greule, Anja
Intra, Bungonsiri
Flemming, Stephan
Rommel, Marcel G. E.
Panbangred, Watanalai
Bechthold, Andreas
The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units
title The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units
title_full The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units
title_fullStr The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units
title_full_unstemmed The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units
title_short The Draft Genome Sequence of Actinokineospora bangkokensis 44EHW(T) Reveals the Biosynthetic Pathway of the Antifungal Thailandin Compounds with Unusual Butylmalonyl-CoA Extender Units
title_sort draft genome sequence of actinokineospora bangkokensis 44ehw(t) reveals the biosynthetic pathway of the antifungal thailandin compounds with unusual butylmalonyl-coa extender units
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273641/
https://www.ncbi.nlm.nih.gov/pubmed/27886115
http://dx.doi.org/10.3390/molecules21111607
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