Cargando…

Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages

In the present investigation, we prepared four different solvent fractions (chloroform, hexane, butanol, and ethyl acetate) of Moringa oleifera extract to evaluate its anti-inflammatory potential and cellular mechanism of action in lipopolysaccharide (LPS)-induced RAW264.7 cells. Cell cytotoxicity a...

Descripción completa

Detalles Bibliográficos
Autores principales: Arulselvan, Palanisamy, Tan, Woan Sean, Gothai, Sivapragasam, Muniandy, Katyakyini, Fakurazi, Sharida, Esa, Norhaizan Mohd, Alarfaj, Abdullah A., Kumar, S. Suresh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273666/
https://www.ncbi.nlm.nih.gov/pubmed/27809259
http://dx.doi.org/10.3390/molecules21111452
_version_ 1783377439988121600
author Arulselvan, Palanisamy
Tan, Woan Sean
Gothai, Sivapragasam
Muniandy, Katyakyini
Fakurazi, Sharida
Esa, Norhaizan Mohd
Alarfaj, Abdullah A.
Kumar, S. Suresh
author_facet Arulselvan, Palanisamy
Tan, Woan Sean
Gothai, Sivapragasam
Muniandy, Katyakyini
Fakurazi, Sharida
Esa, Norhaizan Mohd
Alarfaj, Abdullah A.
Kumar, S. Suresh
author_sort Arulselvan, Palanisamy
collection PubMed
description In the present investigation, we prepared four different solvent fractions (chloroform, hexane, butanol, and ethyl acetate) of Moringa oleifera extract to evaluate its anti-inflammatory potential and cellular mechanism of action in lipopolysaccharide (LPS)-induced RAW264.7 cells. Cell cytotoxicity assay suggested that the solvent fractions were not cytotoxic to macrophages at concentrations up to 200 µg/mL. The ethyl acetate fraction suppressed LPS-induced production of nitric oxide and proinflammatory cytokines in macrophages in a concentration-dependent manner and was more effective than the other fractions. Immunoblot observations revealed that the ethyl acetate fraction effectively inhibited the expression of inflammatory mediators including cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor (NF)-κB p65 through suppression of the NF-κB signaling pathway. Furthermore, it upregulated the expression of the inhibitor of κB (IκBα) and blocked the nuclear translocation of NF-κB. These findings indicated that the ethyl acetate fraction of M. oleifera exhibited potent anti-inflammatory activity in LPS-stimulated macrophages via suppression of the NF-κB signaling pathway.
format Online
Article
Text
id pubmed-6273666
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62736662018-12-28 Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages Arulselvan, Palanisamy Tan, Woan Sean Gothai, Sivapragasam Muniandy, Katyakyini Fakurazi, Sharida Esa, Norhaizan Mohd Alarfaj, Abdullah A. Kumar, S. Suresh Molecules Article In the present investigation, we prepared four different solvent fractions (chloroform, hexane, butanol, and ethyl acetate) of Moringa oleifera extract to evaluate its anti-inflammatory potential and cellular mechanism of action in lipopolysaccharide (LPS)-induced RAW264.7 cells. Cell cytotoxicity assay suggested that the solvent fractions were not cytotoxic to macrophages at concentrations up to 200 µg/mL. The ethyl acetate fraction suppressed LPS-induced production of nitric oxide and proinflammatory cytokines in macrophages in a concentration-dependent manner and was more effective than the other fractions. Immunoblot observations revealed that the ethyl acetate fraction effectively inhibited the expression of inflammatory mediators including cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor (NF)-κB p65 through suppression of the NF-κB signaling pathway. Furthermore, it upregulated the expression of the inhibitor of κB (IκBα) and blocked the nuclear translocation of NF-κB. These findings indicated that the ethyl acetate fraction of M. oleifera exhibited potent anti-inflammatory activity in LPS-stimulated macrophages via suppression of the NF-κB signaling pathway. MDPI 2016-10-31 /pmc/articles/PMC6273666/ /pubmed/27809259 http://dx.doi.org/10.3390/molecules21111452 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arulselvan, Palanisamy
Tan, Woan Sean
Gothai, Sivapragasam
Muniandy, Katyakyini
Fakurazi, Sharida
Esa, Norhaizan Mohd
Alarfaj, Abdullah A.
Kumar, S. Suresh
Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages
title Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages
title_full Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages
title_fullStr Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages
title_full_unstemmed Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages
title_short Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages
title_sort anti-inflammatory potential of ethyl acetate fraction of moringa oleifera in downregulating the nf-κb signaling pathway in lipopolysaccharide-stimulated macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273666/
https://www.ncbi.nlm.nih.gov/pubmed/27809259
http://dx.doi.org/10.3390/molecules21111452
work_keys_str_mv AT arulselvanpalanisamy antiinflammatorypotentialofethylacetatefractionofmoringaoleiferaindownregulatingthenfkbsignalingpathwayinlipopolysaccharidestimulatedmacrophages
AT tanwoansean antiinflammatorypotentialofethylacetatefractionofmoringaoleiferaindownregulatingthenfkbsignalingpathwayinlipopolysaccharidestimulatedmacrophages
AT gothaisivapragasam antiinflammatorypotentialofethylacetatefractionofmoringaoleiferaindownregulatingthenfkbsignalingpathwayinlipopolysaccharidestimulatedmacrophages
AT muniandykatyakyini antiinflammatorypotentialofethylacetatefractionofmoringaoleiferaindownregulatingthenfkbsignalingpathwayinlipopolysaccharidestimulatedmacrophages
AT fakurazisharida antiinflammatorypotentialofethylacetatefractionofmoringaoleiferaindownregulatingthenfkbsignalingpathwayinlipopolysaccharidestimulatedmacrophages
AT esanorhaizanmohd antiinflammatorypotentialofethylacetatefractionofmoringaoleiferaindownregulatingthenfkbsignalingpathwayinlipopolysaccharidestimulatedmacrophages
AT alarfajabdullaha antiinflammatorypotentialofethylacetatefractionofmoringaoleiferaindownregulatingthenfkbsignalingpathwayinlipopolysaccharidestimulatedmacrophages
AT kumarssuresh antiinflammatorypotentialofethylacetatefractionofmoringaoleiferaindownregulatingthenfkbsignalingpathwayinlipopolysaccharidestimulatedmacrophages