Cargando…
Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability
The main purpose of this study was to optimize the preparation of lysozyme nanoliposomes using response surface methodology and measure their stability. The stabilities of lysozyme nanoliposomes in simulated gastrointestinal fluid (SGF), simulated intestinal fluid (SIF), as well as pH, temperature a...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273706/ https://www.ncbi.nlm.nih.gov/pubmed/27338315 http://dx.doi.org/10.3390/molecules21060741 |
_version_ | 1783377449241804800 |
---|---|
author | Wu, Zhipan Guan, Rongfa Lyu, Fei Liu, Mingqi Gao, Jianguo Cao, Guozou |
author_facet | Wu, Zhipan Guan, Rongfa Lyu, Fei Liu, Mingqi Gao, Jianguo Cao, Guozou |
author_sort | Wu, Zhipan |
collection | PubMed |
description | The main purpose of this study was to optimize the preparation of lysozyme nanoliposomes using response surface methodology and measure their stability. The stabilities of lysozyme nanoliposomes in simulated gastrointestinal fluid (SGF), simulated intestinal fluid (SIF), as well as pH, temperature and sonication treatment time were evaluated. Reverse-phase evaporation method is an easy, speedy, and beneficial approach for nanoliposomes’ preparation and optimization. The optimal preparative conditions were as follows: phosphatidylcholine-to-cholesterol ratio of 3.86, lysozyme concentration of 1.96 mg/mL, magnetic stirring time of 40.61 min, and ultrasound time of 14.15 min. At the optimal point, encapsulation efficiency and particle size were found to be 75.36% ± 3.20% and 245.6 nm ± 5.2 nm, respectively. The lysozyme nanoliposomes demonstrated certain stability in SGF and SIF at a temperature of 37 °C for 4 h, and short sonication handling times were required to attain nano-scaled liposomes. Under conditions of high temperature, acidity and alkalinity, lysozyme nanoliposomes are unstable. |
format | Online Article Text |
id | pubmed-6273706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62737062018-12-28 Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability Wu, Zhipan Guan, Rongfa Lyu, Fei Liu, Mingqi Gao, Jianguo Cao, Guozou Molecules Article The main purpose of this study was to optimize the preparation of lysozyme nanoliposomes using response surface methodology and measure their stability. The stabilities of lysozyme nanoliposomes in simulated gastrointestinal fluid (SGF), simulated intestinal fluid (SIF), as well as pH, temperature and sonication treatment time were evaluated. Reverse-phase evaporation method is an easy, speedy, and beneficial approach for nanoliposomes’ preparation and optimization. The optimal preparative conditions were as follows: phosphatidylcholine-to-cholesterol ratio of 3.86, lysozyme concentration of 1.96 mg/mL, magnetic stirring time of 40.61 min, and ultrasound time of 14.15 min. At the optimal point, encapsulation efficiency and particle size were found to be 75.36% ± 3.20% and 245.6 nm ± 5.2 nm, respectively. The lysozyme nanoliposomes demonstrated certain stability in SGF and SIF at a temperature of 37 °C for 4 h, and short sonication handling times were required to attain nano-scaled liposomes. Under conditions of high temperature, acidity and alkalinity, lysozyme nanoliposomes are unstable. MDPI 2016-06-08 /pmc/articles/PMC6273706/ /pubmed/27338315 http://dx.doi.org/10.3390/molecules21060741 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wu, Zhipan Guan, Rongfa Lyu, Fei Liu, Mingqi Gao, Jianguo Cao, Guozou Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability |
title | Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability |
title_full | Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability |
title_fullStr | Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability |
title_full_unstemmed | Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability |
title_short | Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability |
title_sort | optimization of preparation conditions for lysozyme nanoliposomes using response surface methodology and evaluation of their stability |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273706/ https://www.ncbi.nlm.nih.gov/pubmed/27338315 http://dx.doi.org/10.3390/molecules21060741 |
work_keys_str_mv | AT wuzhipan optimizationofpreparationconditionsforlysozymenanoliposomesusingresponsesurfacemethodologyandevaluationoftheirstability AT guanrongfa optimizationofpreparationconditionsforlysozymenanoliposomesusingresponsesurfacemethodologyandevaluationoftheirstability AT lyufei optimizationofpreparationconditionsforlysozymenanoliposomesusingresponsesurfacemethodologyandevaluationoftheirstability AT liumingqi optimizationofpreparationconditionsforlysozymenanoliposomesusingresponsesurfacemethodologyandevaluationoftheirstability AT gaojianguo optimizationofpreparationconditionsforlysozymenanoliposomesusingresponsesurfacemethodologyandevaluationoftheirstability AT caoguozou optimizationofpreparationconditionsforlysozymenanoliposomesusingresponsesurfacemethodologyandevaluationoftheirstability |