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Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study

In the present study we investigated activity of oleuropein, a complex phenol present in large quantities in olive tree products, against opportunistic fungal pathogen Candida albicans. Oleuropein was found to have in vitro antifungal activity with a minimal inhibitory concentration (MIC) value of 1...

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Autores principales: Zorić, Nataša, Kopjar, Nevenka, Bobnjarić, Ivan, Horvat, Igor, Tomić, Siniša, Kosalec, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273721/
https://www.ncbi.nlm.nih.gov/pubmed/27916806
http://dx.doi.org/10.3390/molecules21121631
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author Zorić, Nataša
Kopjar, Nevenka
Bobnjarić, Ivan
Horvat, Igor
Tomić, Siniša
Kosalec, Ivan
author_facet Zorić, Nataša
Kopjar, Nevenka
Bobnjarić, Ivan
Horvat, Igor
Tomić, Siniša
Kosalec, Ivan
author_sort Zorić, Nataša
collection PubMed
description In the present study we investigated activity of oleuropein, a complex phenol present in large quantities in olive tree products, against opportunistic fungal pathogen Candida albicans. Oleuropein was found to have in vitro antifungal activity with a minimal inhibitory concentration (MIC) value of 12.5 mg·mL(−1). Morphological changes in the nuclei after staining with fluorescent DNA-binding dyes revealed that apoptosis was a primary mode of cell death in the analyzed samples treated with subinhibitory concentrations of oleuropein. Our results suggest that this antifungal agent targets virulence factors essential for establishment of the fungal infection. We noticed that oleuropein modulates morphogenetic conversion and inhibits filamentation of C. albicans. The hydrophobicity assay showed that oleuropein in sub-MIC values has significantly decreased, in both aerobic and anaerobic conditions, the cellular surface hydrophobicity (CSH) of C. albicans, a factor associated with adhesion to epithelial cells. It was also demonstrated that the tested compound inhibits the activity of SAPs, cellular enzymes secreted by C. albicans, which are reported to be related to the pathogenicity of the fungi. Additionally, we detected that oleuropein causes a reduction in total sterol content in the membrane of C. albicans cells, which might be involved in the mechanism of its antifungal activity.
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spelling pubmed-62737212018-12-28 Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study Zorić, Nataša Kopjar, Nevenka Bobnjarić, Ivan Horvat, Igor Tomić, Siniša Kosalec, Ivan Molecules Article In the present study we investigated activity of oleuropein, a complex phenol present in large quantities in olive tree products, against opportunistic fungal pathogen Candida albicans. Oleuropein was found to have in vitro antifungal activity with a minimal inhibitory concentration (MIC) value of 12.5 mg·mL(−1). Morphological changes in the nuclei after staining with fluorescent DNA-binding dyes revealed that apoptosis was a primary mode of cell death in the analyzed samples treated with subinhibitory concentrations of oleuropein. Our results suggest that this antifungal agent targets virulence factors essential for establishment of the fungal infection. We noticed that oleuropein modulates morphogenetic conversion and inhibits filamentation of C. albicans. The hydrophobicity assay showed that oleuropein in sub-MIC values has significantly decreased, in both aerobic and anaerobic conditions, the cellular surface hydrophobicity (CSH) of C. albicans, a factor associated with adhesion to epithelial cells. It was also demonstrated that the tested compound inhibits the activity of SAPs, cellular enzymes secreted by C. albicans, which are reported to be related to the pathogenicity of the fungi. Additionally, we detected that oleuropein causes a reduction in total sterol content in the membrane of C. albicans cells, which might be involved in the mechanism of its antifungal activity. MDPI 2016-11-28 /pmc/articles/PMC6273721/ /pubmed/27916806 http://dx.doi.org/10.3390/molecules21121631 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zorić, Nataša
Kopjar, Nevenka
Bobnjarić, Ivan
Horvat, Igor
Tomić, Siniša
Kosalec, Ivan
Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study
title Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study
title_full Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study
title_fullStr Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study
title_full_unstemmed Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study
title_short Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study
title_sort antifungal activity of oleuropein against candida albicans—the in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273721/
https://www.ncbi.nlm.nih.gov/pubmed/27916806
http://dx.doi.org/10.3390/molecules21121631
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