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Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry

To evaluate the nutritional and functional value of Sipunculus nudus, a rapid, simple and sensitive analytical method was developed using ultra-high performance liquid chromatography coupled with a triple quadrupole mass detection in multiple-reaction monitoring mode for the simultaneous quantitativ...

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Autores principales: Ge, Yahui, Tang, Yuping, Guo, Sheng, Liu, Xin, Zhu, Zhenhua, Zhang, Lili, Liu, Pei, Ding, Shaoxiong, Lin, Xiangzhi, Lin, Rurong, Duan, Jin-ao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273726/
https://www.ncbi.nlm.nih.gov/pubmed/27023507
http://dx.doi.org/10.3390/molecules21040408
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author Ge, Yahui
Tang, Yuping
Guo, Sheng
Liu, Xin
Zhu, Zhenhua
Zhang, Lili
Liu, Pei
Ding, Shaoxiong
Lin, Xiangzhi
Lin, Rurong
Duan, Jin-ao
author_facet Ge, Yahui
Tang, Yuping
Guo, Sheng
Liu, Xin
Zhu, Zhenhua
Zhang, Lili
Liu, Pei
Ding, Shaoxiong
Lin, Xiangzhi
Lin, Rurong
Duan, Jin-ao
author_sort Ge, Yahui
collection PubMed
description To evaluate the nutritional and functional value of Sipunculus nudus, a rapid, simple and sensitive analytical method was developed using ultra-high performance liquid chromatography coupled with a triple quadrupole mass detection in multiple-reaction monitoring mode for the simultaneous quantitative determination of 25 free amino acids and 16 nucleosides and nucleobases in S. nudus within 20 min, which was confirmed to be reproducible and accurate. The limits of detection (LODs) and quantification (LOQs) were between 0.003–0.229 μg/mL and 0.008–0.763 μg/mL for the 41 analytes, respectively. The established method was applied to analyze 19 batches of S. nudus samples from four habitats with two different processing methods. The results showed that S. nudus contained a variety of free amino acids, nucleosides and nucleobases in sufficient quantity and reasonable proportion. They also demonstrated that the contents of these compounds in different parts of S. nudus were significantly discriminating, which were in the order: (highest) coelomic fluid > body wall > intestine (lowest). The method is simple and accurate, and could serve as a technical support for establishing quality control of S. nudus and other functional seafoods. Moreover, the research results also laid foundation for further exploitation and development of S. nudus.
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spelling pubmed-62737262018-12-28 Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry Ge, Yahui Tang, Yuping Guo, Sheng Liu, Xin Zhu, Zhenhua Zhang, Lili Liu, Pei Ding, Shaoxiong Lin, Xiangzhi Lin, Rurong Duan, Jin-ao Molecules Article To evaluate the nutritional and functional value of Sipunculus nudus, a rapid, simple and sensitive analytical method was developed using ultra-high performance liquid chromatography coupled with a triple quadrupole mass detection in multiple-reaction monitoring mode for the simultaneous quantitative determination of 25 free amino acids and 16 nucleosides and nucleobases in S. nudus within 20 min, which was confirmed to be reproducible and accurate. The limits of detection (LODs) and quantification (LOQs) were between 0.003–0.229 μg/mL and 0.008–0.763 μg/mL for the 41 analytes, respectively. The established method was applied to analyze 19 batches of S. nudus samples from four habitats with two different processing methods. The results showed that S. nudus contained a variety of free amino acids, nucleosides and nucleobases in sufficient quantity and reasonable proportion. They also demonstrated that the contents of these compounds in different parts of S. nudus were significantly discriminating, which were in the order: (highest) coelomic fluid > body wall > intestine (lowest). The method is simple and accurate, and could serve as a technical support for establishing quality control of S. nudus and other functional seafoods. Moreover, the research results also laid foundation for further exploitation and development of S. nudus. MDPI 2016-03-25 /pmc/articles/PMC6273726/ /pubmed/27023507 http://dx.doi.org/10.3390/molecules21040408 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ge, Yahui
Tang, Yuping
Guo, Sheng
Liu, Xin
Zhu, Zhenhua
Zhang, Lili
Liu, Pei
Ding, Shaoxiong
Lin, Xiangzhi
Lin, Rurong
Duan, Jin-ao
Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry
title Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry
title_full Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry
title_fullStr Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry
title_full_unstemmed Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry
title_short Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry
title_sort simultaneous quantitation of free amino acids, nucleosides and nucleobases in sipunculus nudus by ultra-high performance liquid chromatography with triple quadrupole mass spectrometry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273726/
https://www.ncbi.nlm.nih.gov/pubmed/27023507
http://dx.doi.org/10.3390/molecules21040408
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