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Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages

The tung tree (Vernicia fordii), a non-model woody plant belonging to the Euphorbiaceae family, is a promising economic plant due to the high content of a novel high-value oil in its seeds. Many metabolic pathways are active during seed development. Oil (triacylglycerols (TAGs)) accumulates in oil b...

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Detalles Bibliográficos
Autores principales: Zhan, Zhiyong, Chen, Yicun, Shockey, Jay, Han, Xiaojiao, Wang, Yangdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273751/
https://www.ncbi.nlm.nih.gov/pubmed/27834836
http://dx.doi.org/10.3390/molecules21111486
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author Zhan, Zhiyong
Chen, Yicun
Shockey, Jay
Han, Xiaojiao
Wang, Yangdong
author_facet Zhan, Zhiyong
Chen, Yicun
Shockey, Jay
Han, Xiaojiao
Wang, Yangdong
author_sort Zhan, Zhiyong
collection PubMed
description The tung tree (Vernicia fordii), a non-model woody plant belonging to the Euphorbiaceae family, is a promising economic plant due to the high content of a novel high-value oil in its seeds. Many metabolic pathways are active during seed development. Oil (triacylglycerols (TAGs)) accumulates in oil bodies distributed in the endosperm cells of tung tree seeds. The relationship between oil bodies and oil content during tung tree seed development was analyzed using ultrastructural observations, which confirmed that oil accumulation was correlated with the volumes and numbers of oil bodies in the endosperm cells during three different developmental stages. For a deeper understanding of seed development, we carried out proteomic analyses. At least 144 proteins were differentially expressed during three different developmental stages. A total of 76 proteins were successfully identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry/mass spectrometry (MALDI-TOF/MS/MS). These proteins were grouped into 11 classes according to their functions. The major groups of differentially expressed proteins were associated with energy metabolism (25%), fatty acid metabolism (15.79%) and defense (14.47%). These results strongly suggested that a very high percentage of gene expression in seed development is dedicated to the synthesis and accumulation of TAGs.
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spelling pubmed-62737512018-12-28 Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages Zhan, Zhiyong Chen, Yicun Shockey, Jay Han, Xiaojiao Wang, Yangdong Molecules Article The tung tree (Vernicia fordii), a non-model woody plant belonging to the Euphorbiaceae family, is a promising economic plant due to the high content of a novel high-value oil in its seeds. Many metabolic pathways are active during seed development. Oil (triacylglycerols (TAGs)) accumulates in oil bodies distributed in the endosperm cells of tung tree seeds. The relationship between oil bodies and oil content during tung tree seed development was analyzed using ultrastructural observations, which confirmed that oil accumulation was correlated with the volumes and numbers of oil bodies in the endosperm cells during three different developmental stages. For a deeper understanding of seed development, we carried out proteomic analyses. At least 144 proteins were differentially expressed during three different developmental stages. A total of 76 proteins were successfully identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry/mass spectrometry (MALDI-TOF/MS/MS). These proteins were grouped into 11 classes according to their functions. The major groups of differentially expressed proteins were associated with energy metabolism (25%), fatty acid metabolism (15.79%) and defense (14.47%). These results strongly suggested that a very high percentage of gene expression in seed development is dedicated to the synthesis and accumulation of TAGs. MDPI 2016-11-08 /pmc/articles/PMC6273751/ /pubmed/27834836 http://dx.doi.org/10.3390/molecules21111486 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhan, Zhiyong
Chen, Yicun
Shockey, Jay
Han, Xiaojiao
Wang, Yangdong
Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages
title Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages
title_full Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages
title_fullStr Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages
title_full_unstemmed Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages
title_short Proteomic Analysis of Tung Tree (Vernicia fordii) Oilseeds during the Developmental Stages
title_sort proteomic analysis of tung tree (vernicia fordii) oilseeds during the developmental stages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273751/
https://www.ncbi.nlm.nih.gov/pubmed/27834836
http://dx.doi.org/10.3390/molecules21111486
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