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The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr

The amount of secondary metabolites in plants can be enhanced or reduced by various external factors. In this study, the effect of strontium ions on the production of phytoestrogens in soybeans was investigated. The plants were treated with Hoagland’s solution, modified with Sr(2+) with concentratio...

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Autores principales: Wójciak-Kosior, Magdalena, Sowa, Ireneusz, Blicharski, Tomasz, Strzemski, Maciej, Dresler, Sławomir, Szymczak, Grażyna, Wnorowski, Artur, Kocjan, Ryszard, Świeboda, Ryszard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273764/
https://www.ncbi.nlm.nih.gov/pubmed/26784151
http://dx.doi.org/10.3390/molecules21010090
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author Wójciak-Kosior, Magdalena
Sowa, Ireneusz
Blicharski, Tomasz
Strzemski, Maciej
Dresler, Sławomir
Szymczak, Grażyna
Wnorowski, Artur
Kocjan, Ryszard
Świeboda, Ryszard
author_facet Wójciak-Kosior, Magdalena
Sowa, Ireneusz
Blicharski, Tomasz
Strzemski, Maciej
Dresler, Sławomir
Szymczak, Grażyna
Wnorowski, Artur
Kocjan, Ryszard
Świeboda, Ryszard
author_sort Wójciak-Kosior, Magdalena
collection PubMed
description The amount of secondary metabolites in plants can be enhanced or reduced by various external factors. In this study, the effect of strontium ions on the production of phytoestrogens in soybeans was investigated. The plants were treated with Hoagland’s solution, modified with Sr(2+) with concentrations ranging from 0.5 to 3.0 mM, and were grown for 14 days in hydroponic cultivation. After harvest, soybean plants were separated into roots and shoots, dried, and pulverized. The plant material was extracted with methanol and hydrolyzed. Phytoestrogens were quantified by HPLC. The significant increase in the concentration of the compounds of interest was observed for all tested concentrations of strontium ions when compared to control. Sr(2+) at a concentration of 2 mM was the strongest elicitor, and the amount of phytoestrogens in plant increased ca. 2.70, 1.92, 3.77 and 2.88-fold, for daidzein, coumestrol, genistein and formononetin, respectively. Moreover, no cytotoxic effects were observed in HepG2 liver cell models after treatment with extracts from 2 mM Sr(2+)-stressed soybean plants when compared to extracts from non-stressed plants. Our results indicate that the addition of strontium ions to the culture media may be used to functionalize soybean plants with enhanced phytoestrogen content.
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spelling pubmed-62737642018-12-28 The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr Wójciak-Kosior, Magdalena Sowa, Ireneusz Blicharski, Tomasz Strzemski, Maciej Dresler, Sławomir Szymczak, Grażyna Wnorowski, Artur Kocjan, Ryszard Świeboda, Ryszard Molecules Article The amount of secondary metabolites in plants can be enhanced or reduced by various external factors. In this study, the effect of strontium ions on the production of phytoestrogens in soybeans was investigated. The plants were treated with Hoagland’s solution, modified with Sr(2+) with concentrations ranging from 0.5 to 3.0 mM, and were grown for 14 days in hydroponic cultivation. After harvest, soybean plants were separated into roots and shoots, dried, and pulverized. The plant material was extracted with methanol and hydrolyzed. Phytoestrogens were quantified by HPLC. The significant increase in the concentration of the compounds of interest was observed for all tested concentrations of strontium ions when compared to control. Sr(2+) at a concentration of 2 mM was the strongest elicitor, and the amount of phytoestrogens in plant increased ca. 2.70, 1.92, 3.77 and 2.88-fold, for daidzein, coumestrol, genistein and formononetin, respectively. Moreover, no cytotoxic effects were observed in HepG2 liver cell models after treatment with extracts from 2 mM Sr(2+)-stressed soybean plants when compared to extracts from non-stressed plants. Our results indicate that the addition of strontium ions to the culture media may be used to functionalize soybean plants with enhanced phytoestrogen content. MDPI 2016-01-14 /pmc/articles/PMC6273764/ /pubmed/26784151 http://dx.doi.org/10.3390/molecules21010090 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wójciak-Kosior, Magdalena
Sowa, Ireneusz
Blicharski, Tomasz
Strzemski, Maciej
Dresler, Sławomir
Szymczak, Grażyna
Wnorowski, Artur
Kocjan, Ryszard
Świeboda, Ryszard
The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr
title The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr
title_full The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr
title_fullStr The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr
title_full_unstemmed The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr
title_short The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr
title_sort stimulatory effect of strontium ions on phytoestrogens content in glycine max (l.) merr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273764/
https://www.ncbi.nlm.nih.gov/pubmed/26784151
http://dx.doi.org/10.3390/molecules21010090
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