Cargando…

Methods for Improving Aptamer Binding Affinity

Aptamers are single stranded oligonucleotides that bind a wide range of biological targets. Although aptamers can be isolated from pools of random sequence oligonucleotides using affinity-based selection, aptamers with high affinities are not always obtained. Therefore, further refinement of aptamer...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hasegawa, Hijiri, Savory, Nasa, Abe, Koichi, Ikebukuro, Kazunori
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2016
Materias:	Review
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273865/ https://www.ncbi.nlm.nih.gov/pubmed/27043498 http://dx.doi.org/10.3390/molecules21040421

Ejemplares similares

Improvement of Aptamer Affinity by Dimerization
por: Hasegawa, Hijiri, et al.
Publicado: (2008)

Control of Aptamer Function Using Radiofrequency Magnetic Field
por: Taira, Kenichi, et al.
Publicado: (2011)

Screening and Improvement of an Anti-VEGF DNA Aptamer
por: Nonaka, Yoshihiko, et al.
Publicado: (2010)

Vascular Endothelial Growth Factor (VEGF) Detection Using an Aptamer and PNA-Based Bound/Free Separation System
por: Mita, Chifuku, et al.
Publicado: (2014)

G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol
por: Tsukakoshi, Kaori, et al.
Publicado: (2021)

A novel method of screening thrombin-inhibiting DNA aptamers using an evolution-mimicking algorithm
por: Ikebukuro, Kazunori, et al.
Publicado: (2005)

Application of a Glucose Dehydrogenase-Fused with Zinc Finger Protein to Label DNA Aptamers for the Electrochemical Detection of VEGF
por: Lee, Jinhee, et al.
Publicado: (2020)

Homogeneous DNA sensing using enzyme-inhibiting DNA aptamers
por: Yoshida, Wataru, et al.
Publicado: (2006)

Identification of RNA Oligonucleotides Binding to Several Proteins from Potential G-Quadruplex Forming Regions in Transcribed Pre-mRNA
por: Saito, Taiki, et al.
Publicado: (2015)

Aptamer Selection Based on G4-Forming Promoter Region
por: Yoshida, Wataru, et al.
Publicado: (2013)

Detection of CpG Methylation in G-Quadruplex Forming Sequences Using G-Quadruplex Ligands
por: Hasegawa, Hijiri, et al.
Publicado: (2021)

Anti-Idiotype DNA Aptamer Affinity Purification–High-Temperature Reversed-Phase Liquid Chromatography: A Simple, Accurate, and Selective Bioanalysis of Bevacizumab
por: Yamada, Tomohiro, et al.
Publicado: (2019)

Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects
por: Elskens, Jan P., et al.
Publicado: (2020)

Identification of two aptamers binding to Legionella pneumophila with high affinity and specificity
por: Saad, Mariam, et al.
Publicado: (2020)

Development of an Anti-Idiotype Aptamer-Based Electrochemical Sensor for a Humanized Therapeutic Antibody Monitoring
por: Nagata, Madoka, et al.
Publicado: (2023)

Chemically modified aptamers for improving binding affinity to the target proteins via enhanced non-covalent bonding
por: Chen, Zefeng, et al.
Publicado: (2023)

An Amine-Reactive Phenazine Ethosulfate (arPES)—A Novel Redox Probe for Electrochemical Aptamer-Based Sensor
por: Nagata, Madoka, et al.
Publicado: (2022)

Peptide ligand screening of α-synuclein aggregation modulators by in silico panning
por: Abe, Koichi, et al.
Publicado: (2007)

Rationally Manipulating Aptamer Binding Affinities in a Stem-Loop Molecular Beacon
por: Armstrong, Rachel E., et al.
Publicado: (2014)

Selecting RNA aptamers for synthetic biology: investigating magnesium dependence and predicting binding affinity
por: Carothers, James M., et al.
Publicado: (2010)

Development of an aptamer-based affinity purification method for vascular endothelial growth factor
por: Lönne, Maren, et al.
Publicado: (2015)

Direct Detection of Thrombin Binding to 8-Bromodeoxyguanosine-Modified Aptamer: Effects of Modification on Affinity and Kinetics
por: Goji, Shou, et al.
Publicado: (2011)

Characterisation of aptamer-anchored poly(EDMA-co-GMA) monolith for high throughput affinity binding
por: Acquah, Caleb, et al.
Publicado: (2019)

The Influence of Protein Charge and Molecular Weight on the Affinity of Aptamers
por: Drees, Alissa, et al.
Publicado: (2023)

Artificial Intelligence in Aptamer–Target Binding Prediction
por: Chen, Zihao, et al.
Publicado: (2021)

Recent Advances in Identifying Biomarkers and High-Affinity Aptamers for Gynecologic Cancers Diagnosis and Therapy
por: Ma, Xiaoqun, et al.
Publicado: (2019)

Identification and Affinity Determination of Protein-Antibody and Protein-Aptamer Epitopes by Biosensor-Mass Spectrometry Combination †
por: Lupu, Loredana-Mirela, et al.
Publicado: (2021)

Selection of 2′-deoxy-2′-fluoroarabinonucleotide (FANA) aptamers that bind HIV-1 reverse transcriptase with picomolar affinity
por: Alves Ferreira-Bravo, Irani, et al.
Publicado: (2015)

Mapping the affinity landscape of Thrombin-binding aptamers on 2΄F-ANA/DNA chimeric G-Quadruplex microarrays
por: Lietard, Jory, et al.
Publicado: (2017)

Integrative structural biology studies of HIV-1 reverse transcriptase binding to a high-affinity DNA aptamer
por: Tuske, Steve, et al.
Publicado: (2020)

PEI-assisted boronate affinity magnetic nanoparticle-based SELEX for efficient in vitro evolution of saponin-binding aptamers
por: Zhang, Hui, et al.
Publicado: (2021)

Microarrays as Model Biosensor Platforms to Investigate the Structure and Affinity of Aptamers
por: Martin, Jennifer A., et al.
Publicado: (2016)

Probing the Limits of Aptamer Affinity with a Microfluidic SELEX Platform
por: Ahmad, Kareem M., et al.
Publicado: (2011)

Target Affinity and Structural Analysis for a Selection of Norovirus Aptamers
por: Schilling-Loeffler, Katja, et al.
Publicado: (2021)

The MM/PBSA and MM/GBSA methods to estimate ligand-binding affinities
por: Genheden, Samuel, et al.
Publicado: (2015)

A Sensitive Aptamer Fluorescence Anisotropy Sensor for Cd(2+) Using Affinity-Enhanced Aptamers with Phosphorothioate Modification
por: Yu, Hao, et al.
Publicado: (2022)

Engineering of a green-light inducible gene expression system in Synechocystis sp. PCC6803
por: Abe, Koichi, et al.
Publicado: (2014)

The importance of helix P1 stability for structural pre-organization and ligand binding affinity of the adenine riboswitch aptamer domain
por: Nozinovic, Senada, et al.
Publicado: (2014)

Selection of 2′-Deoxy-2′-Fluoroarabino Nucleic Acid (FANA) Aptamers That Bind HIV-1 Integrase with Picomolar Affinity
por: Rose, Kevin M., et al.
Publicado: (2019)

What defines a synthetic riboswitch? – Conformational dynamics of ciprofloxacin aptamers with similar binding affinities but varying regulatory potentials
por: Kaiser, Christoph, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_59ibtt09oiomr1p5j2s7l562vc