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Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion

Background: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min...

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Autores principales: Chamorro, Virginia, Pandolfi, Rachele, Moreno, Laura, Barreira, Bianca, Martínez-Ramas, Andrea, Morales-Cano, Daniel, Ruiz-Cabello, Jesús, Lorente, José Angel, Duarte, Juan, Cogolludo, Ángel, Alvarez-Sala, José Luis, Perez-Vizcaino, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273949/
https://www.ncbi.nlm.nih.gov/pubmed/27999410
http://dx.doi.org/10.3390/molecules21121739
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author Chamorro, Virginia
Pandolfi, Rachele
Moreno, Laura
Barreira, Bianca
Martínez-Ramas, Andrea
Morales-Cano, Daniel
Ruiz-Cabello, Jesús
Lorente, José Angel
Duarte, Juan
Cogolludo, Ángel
Alvarez-Sala, José Luis
Perez-Vizcaino, Francisco
author_facet Chamorro, Virginia
Pandolfi, Rachele
Moreno, Laura
Barreira, Bianca
Martínez-Ramas, Andrea
Morales-Cano, Daniel
Ruiz-Cabello, Jesús
Lorente, José Angel
Duarte, Juan
Cogolludo, Ángel
Alvarez-Sala, José Luis
Perez-Vizcaino, Francisco
author_sort Chamorro, Virginia
collection PubMed
description Background: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min (trauma), hemorrhagic shock (blood withdrawal to reduce the mean arterial pressure to 35 mmHg) for 75 min and resuscitation by re-infusion of all the shed blood plus lactate Ringer for 90 min. Intravenous quercetin (50 mg/kg) or vehicle were administered during resuscitation. Results: There was a trend for increased survival 84.6% (11/13) in the treated group vs. the shock group 68.4% (13/19, p > 0.05 Kaplan–Meier). Quercetin fully prevented the development of lung edema. The activity of aSMase was increased in the shock group compared to the sham group and the quercetin prevented this effect. However, other inflammatory markers such as myeloperoxidase activity, interleukin-6 in plasma or bronchoalveolar fluid were similar in the sham and shock groups. We found no bacterial DNA in plasma in these animals. Conclusions: Quercetin partially prevented the changes in blood pressure and lung injury in shock associated to hemorrhage and reperfusion.
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spelling pubmed-62739492018-12-28 Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion Chamorro, Virginia Pandolfi, Rachele Moreno, Laura Barreira, Bianca Martínez-Ramas, Andrea Morales-Cano, Daniel Ruiz-Cabello, Jesús Lorente, José Angel Duarte, Juan Cogolludo, Ángel Alvarez-Sala, José Luis Perez-Vizcaino, Francisco Molecules Article Background: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min (trauma), hemorrhagic shock (blood withdrawal to reduce the mean arterial pressure to 35 mmHg) for 75 min and resuscitation by re-infusion of all the shed blood plus lactate Ringer for 90 min. Intravenous quercetin (50 mg/kg) or vehicle were administered during resuscitation. Results: There was a trend for increased survival 84.6% (11/13) in the treated group vs. the shock group 68.4% (13/19, p > 0.05 Kaplan–Meier). Quercetin fully prevented the development of lung edema. The activity of aSMase was increased in the shock group compared to the sham group and the quercetin prevented this effect. However, other inflammatory markers such as myeloperoxidase activity, interleukin-6 in plasma or bronchoalveolar fluid were similar in the sham and shock groups. We found no bacterial DNA in plasma in these animals. Conclusions: Quercetin partially prevented the changes in blood pressure and lung injury in shock associated to hemorrhage and reperfusion. MDPI 2016-12-20 /pmc/articles/PMC6273949/ /pubmed/27999410 http://dx.doi.org/10.3390/molecules21121739 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chamorro, Virginia
Pandolfi, Rachele
Moreno, Laura
Barreira, Bianca
Martínez-Ramas, Andrea
Morales-Cano, Daniel
Ruiz-Cabello, Jesús
Lorente, José Angel
Duarte, Juan
Cogolludo, Ángel
Alvarez-Sala, José Luis
Perez-Vizcaino, Francisco
Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion
title Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion
title_full Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion
title_fullStr Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion
title_full_unstemmed Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion
title_short Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion
title_sort effects of quercetin in a rat model of hemorrhagic traumatic shock and reperfusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273949/
https://www.ncbi.nlm.nih.gov/pubmed/27999410
http://dx.doi.org/10.3390/molecules21121739
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