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Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion
Background: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273949/ https://www.ncbi.nlm.nih.gov/pubmed/27999410 http://dx.doi.org/10.3390/molecules21121739 |
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author | Chamorro, Virginia Pandolfi, Rachele Moreno, Laura Barreira, Bianca Martínez-Ramas, Andrea Morales-Cano, Daniel Ruiz-Cabello, Jesús Lorente, José Angel Duarte, Juan Cogolludo, Ángel Alvarez-Sala, José Luis Perez-Vizcaino, Francisco |
author_facet | Chamorro, Virginia Pandolfi, Rachele Moreno, Laura Barreira, Bianca Martínez-Ramas, Andrea Morales-Cano, Daniel Ruiz-Cabello, Jesús Lorente, José Angel Duarte, Juan Cogolludo, Ángel Alvarez-Sala, José Luis Perez-Vizcaino, Francisco |
author_sort | Chamorro, Virginia |
collection | PubMed |
description | Background: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min (trauma), hemorrhagic shock (blood withdrawal to reduce the mean arterial pressure to 35 mmHg) for 75 min and resuscitation by re-infusion of all the shed blood plus lactate Ringer for 90 min. Intravenous quercetin (50 mg/kg) or vehicle were administered during resuscitation. Results: There was a trend for increased survival 84.6% (11/13) in the treated group vs. the shock group 68.4% (13/19, p > 0.05 Kaplan–Meier). Quercetin fully prevented the development of lung edema. The activity of aSMase was increased in the shock group compared to the sham group and the quercetin prevented this effect. However, other inflammatory markers such as myeloperoxidase activity, interleukin-6 in plasma or bronchoalveolar fluid were similar in the sham and shock groups. We found no bacterial DNA in plasma in these animals. Conclusions: Quercetin partially prevented the changes in blood pressure and lung injury in shock associated to hemorrhage and reperfusion. |
format | Online Article Text |
id | pubmed-6273949 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62739492018-12-28 Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion Chamorro, Virginia Pandolfi, Rachele Moreno, Laura Barreira, Bianca Martínez-Ramas, Andrea Morales-Cano, Daniel Ruiz-Cabello, Jesús Lorente, José Angel Duarte, Juan Cogolludo, Ángel Alvarez-Sala, José Luis Perez-Vizcaino, Francisco Molecules Article Background: We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock. Methods: Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min (trauma), hemorrhagic shock (blood withdrawal to reduce the mean arterial pressure to 35 mmHg) for 75 min and resuscitation by re-infusion of all the shed blood plus lactate Ringer for 90 min. Intravenous quercetin (50 mg/kg) or vehicle were administered during resuscitation. Results: There was a trend for increased survival 84.6% (11/13) in the treated group vs. the shock group 68.4% (13/19, p > 0.05 Kaplan–Meier). Quercetin fully prevented the development of lung edema. The activity of aSMase was increased in the shock group compared to the sham group and the quercetin prevented this effect. However, other inflammatory markers such as myeloperoxidase activity, interleukin-6 in plasma or bronchoalveolar fluid were similar in the sham and shock groups. We found no bacterial DNA in plasma in these animals. Conclusions: Quercetin partially prevented the changes in blood pressure and lung injury in shock associated to hemorrhage and reperfusion. MDPI 2016-12-20 /pmc/articles/PMC6273949/ /pubmed/27999410 http://dx.doi.org/10.3390/molecules21121739 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chamorro, Virginia Pandolfi, Rachele Moreno, Laura Barreira, Bianca Martínez-Ramas, Andrea Morales-Cano, Daniel Ruiz-Cabello, Jesús Lorente, José Angel Duarte, Juan Cogolludo, Ángel Alvarez-Sala, José Luis Perez-Vizcaino, Francisco Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
title | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
title_full | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
title_fullStr | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
title_full_unstemmed | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
title_short | Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion |
title_sort | effects of quercetin in a rat model of hemorrhagic traumatic shock and reperfusion |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6273949/ https://www.ncbi.nlm.nih.gov/pubmed/27999410 http://dx.doi.org/10.3390/molecules21121739 |
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