A Method for Determining the Content of Glycoproteins in Biological Samples

The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectromet...

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Autores principales: Gao, Yang, Xu, Duoduo, Li, Hongyue, Yang, Xianling, Wang, Mingxing, Gao, Qipin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274010/
https://www.ncbi.nlm.nih.gov/pubmed/27898038
http://dx.doi.org/10.3390/molecules21121625
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author Gao, Yang
Xu, Duoduo
Li, Hongyue
Yang, Xianling
Wang, Mingxing
Gao, Qipin
author_facet Gao, Yang
Xu, Duoduo
Li, Hongyue
Yang, Xianling
Wang, Mingxing
Gao, Qipin
author_sort Gao, Yang
collection PubMed
description The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs.
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spelling pubmed-62740102018-12-28 A Method for Determining the Content of Glycoproteins in Biological Samples Gao, Yang Xu, Duoduo Li, Hongyue Yang, Xianling Wang, Mingxing Gao, Qipin Molecules Article The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs. MDPI 2016-11-26 /pmc/articles/PMC6274010/ /pubmed/27898038 http://dx.doi.org/10.3390/molecules21121625 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gao, Yang
Xu, Duoduo
Li, Hongyue
Yang, Xianling
Wang, Mingxing
Gao, Qipin
A Method for Determining the Content of Glycoproteins in Biological Samples
title A Method for Determining the Content of Glycoproteins in Biological Samples
title_full A Method for Determining the Content of Glycoproteins in Biological Samples
title_fullStr A Method for Determining the Content of Glycoproteins in Biological Samples
title_full_unstemmed A Method for Determining the Content of Glycoproteins in Biological Samples
title_short A Method for Determining the Content of Glycoproteins in Biological Samples
title_sort method for determining the content of glycoproteins in biological samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274010/
https://www.ncbi.nlm.nih.gov/pubmed/27898038
http://dx.doi.org/10.3390/molecules21121625
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