Identification and Characterization of Two New Degradation Products of Saikosaponin A under Acid Hydrolytic Conditions

Saikosaponin (SS) A is a compound with various pharmacological properties and is easily degraded into SS-B1 and SS-G under acid conditions. In the present work, two new degradation products of SS-A, formed under acid hydrolytic conditions, were detected and isolated using analytical and semi-prepara...

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Detalles Bibliográficos
Autores principales: Li, Jun, Xu, Qiang, Jiang, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274035/
https://www.ncbi.nlm.nih.gov/pubmed/27649123
http://dx.doi.org/10.3390/molecules21091232
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author Li, Jun
Xu, Qiang
Jiang, Hua
author_facet Li, Jun
Xu, Qiang
Jiang, Hua
author_sort Li, Jun
collection PubMed
description Saikosaponin (SS) A is a compound with various pharmacological properties and is easily degraded into SS-B1 and SS-G under acid conditions. In the present work, two new degradation products of SS-A, formed under acid hydrolytic conditions, were detected and isolated using analytical and semi-preparative liquid chromatography technology; furthermore, their structures were characterized as hydroxy-saikosaponin A and SS-B2 by spectral analysis, which is not only essential in stability-indicating method development and validation, but also could be used as a worst case scenario to assess the analytical method performance of SS-A. Moreover, their structural transformation pathways are also proposed.
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spelling pubmed-62740352018-12-28 Identification and Characterization of Two New Degradation Products of Saikosaponin A under Acid Hydrolytic Conditions Li, Jun Xu, Qiang Jiang, Hua Molecules Article Saikosaponin (SS) A is a compound with various pharmacological properties and is easily degraded into SS-B1 and SS-G under acid conditions. In the present work, two new degradation products of SS-A, formed under acid hydrolytic conditions, were detected and isolated using analytical and semi-preparative liquid chromatography technology; furthermore, their structures were characterized as hydroxy-saikosaponin A and SS-B2 by spectral analysis, which is not only essential in stability-indicating method development and validation, but also could be used as a worst case scenario to assess the analytical method performance of SS-A. Moreover, their structural transformation pathways are also proposed. MDPI 2016-09-14 /pmc/articles/PMC6274035/ /pubmed/27649123 http://dx.doi.org/10.3390/molecules21091232 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Jun
Xu, Qiang
Jiang, Hua
Identification and Characterization of Two New Degradation Products of Saikosaponin A under Acid Hydrolytic Conditions
title Identification and Characterization of Two New Degradation Products of Saikosaponin A under Acid Hydrolytic Conditions
title_full Identification and Characterization of Two New Degradation Products of Saikosaponin A under Acid Hydrolytic Conditions
title_fullStr Identification and Characterization of Two New Degradation Products of Saikosaponin A under Acid Hydrolytic Conditions
title_full_unstemmed Identification and Characterization of Two New Degradation Products of Saikosaponin A under Acid Hydrolytic Conditions
title_short Identification and Characterization of Two New Degradation Products of Saikosaponin A under Acid Hydrolytic Conditions
title_sort identification and characterization of two new degradation products of saikosaponin a under acid hydrolytic conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274035/
https://www.ncbi.nlm.nih.gov/pubmed/27649123
http://dx.doi.org/10.3390/molecules21091232
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AT xuqiang identificationandcharacterizationoftwonewdegradationproductsofsaikosaponinaunderacidhydrolyticconditions
AT jianghua identificationandcharacterizationoftwonewdegradationproductsofsaikosaponinaunderacidhydrolyticconditions

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