DNA Three Way Junction Core Decorated with Amino Acids-Like Residues-Synthesis and Characterization †

Construction and physico-chemical behavior of DNA three way junction (3WJ) functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 5′-C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react...

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Detalles Bibliográficos
Autores principales: Addamiano, Claudia, Gerland, Béatrice, Payrastre, Corinne, Escudier, Jean-Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274049/
https://www.ncbi.nlm.nih.gov/pubmed/27563857
http://dx.doi.org/10.3390/molecules21091082
Descripción
Sumario:Construction and physico-chemical behavior of DNA three way junction (3WJ) functionalized by protein-like residues (imidazole, alcohol and carboxylic acid) at unpaired positions at the core is described. One 5′-C(S)-propargyl-thymidine nucleotide was specifically incorporated on each strand to react through a post synthetic CuACC reaction with either protected imidazolyl-, hydroxyl- or carboxyl-azide. Structural impacts of 5′-C(S)-functionalization were investigated to evaluate how 3WJ flexibility/stability is affected.

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