Site-Selective Ribosylation of Fluorescent Nucleobase Analogs Using Purine-Nucleoside Phosphorylase as a Catalyst: Effects of Point Mutations

Enzymatic ribosylation of fluorescent 8-azapurine derivatives, like 8-azaguanine and 2,6-diamino-8-azapurine, with purine-nucleoside phosphorylase (PNP) as a catalyst, leads to N9, N8, and N7-ribosides. The final proportion of the products may be modulated by point mutations in the enzyme active sit...

Descripción completa

Detalles Bibliográficos
Autores principales: Stachelska-Wierzchowska, Alicja, Wierzchowski, Jacek, Bzowska, Agnieszka, Wielgus-Kutrowska, Beata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274182/
https://www.ncbi.nlm.nih.gov/pubmed/26729076
http://dx.doi.org/10.3390/molecules21010044

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274182/
https://www.ncbi.nlm.nih.gov/pubmed/26729076
http://dx.doi.org/10.3390/molecules21010044

Ejemplares similares