Cargando…
Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs
Background: Rhaphiodon echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characteri...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274211/ https://www.ncbi.nlm.nih.gov/pubmed/27338314 http://dx.doi.org/10.3390/molecules21060743 |
_version_ | 1783377566253449216 |
---|---|
author | Duarte, Antonia Eliene de Menezes, Irwin Rose Alencar Bezerra Morais Braga, Maria Flaviana Leite, Nadghia Figueiredo Barros, Luiz Marivando Waczuk, Emily Pansera Pessoa da Silva, Maria Arlene Boligon, Aline Teixeira Rocha, João Batista Souza, Diogo Onofre Kamdem, Jean Paul Melo Coutinho, Henrique Douglas Escobar Burger, Marilise |
author_facet | Duarte, Antonia Eliene de Menezes, Irwin Rose Alencar Bezerra Morais Braga, Maria Flaviana Leite, Nadghia Figueiredo Barros, Luiz Marivando Waczuk, Emily Pansera Pessoa da Silva, Maria Arlene Boligon, Aline Teixeira Rocha, João Batista Souza, Diogo Onofre Kamdem, Jean Paul Melo Coutinho, Henrique Douglas Escobar Burger, Marilise |
author_sort | Duarte, Antonia Eliene |
collection | PubMed |
description | Background: Rhaphiodon echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs. |
format | Online Article Text |
id | pubmed-6274211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62742112018-12-28 Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs Duarte, Antonia Eliene de Menezes, Irwin Rose Alencar Bezerra Morais Braga, Maria Flaviana Leite, Nadghia Figueiredo Barros, Luiz Marivando Waczuk, Emily Pansera Pessoa da Silva, Maria Arlene Boligon, Aline Teixeira Rocha, João Batista Souza, Diogo Onofre Kamdem, Jean Paul Melo Coutinho, Henrique Douglas Escobar Burger, Marilise Molecules Article Background: Rhaphiodon echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs. MDPI 2016-06-08 /pmc/articles/PMC6274211/ /pubmed/27338314 http://dx.doi.org/10.3390/molecules21060743 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Duarte, Antonia Eliene de Menezes, Irwin Rose Alencar Bezerra Morais Braga, Maria Flaviana Leite, Nadghia Figueiredo Barros, Luiz Marivando Waczuk, Emily Pansera Pessoa da Silva, Maria Arlene Boligon, Aline Teixeira Rocha, João Batista Souza, Diogo Onofre Kamdem, Jean Paul Melo Coutinho, Henrique Douglas Escobar Burger, Marilise Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs |
title | Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs |
title_full | Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs |
title_fullStr | Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs |
title_full_unstemmed | Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs |
title_short | Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs |
title_sort | antimicrobial activity and modulatory effect of essential oil from the leaf of rhaphiodon echinus (nees & mart) schauer on some antimicrobial drugs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274211/ https://www.ncbi.nlm.nih.gov/pubmed/27338314 http://dx.doi.org/10.3390/molecules21060743 |
work_keys_str_mv | AT duarteantoniaeliene antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT demenezesirwinrosealencar antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT bezerramoraisbragamariaflaviana antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT leitenadghiafigueiredo antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT barrosluizmarivando antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT waczukemilypansera antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT pessoadasilvamariaarlene antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT boligonaline antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT teixeirarochajoaobatista antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT souzadiogoonofre antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT kamdemjeanpaul antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT melocoutinhohenriquedouglas antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs AT escobarburgermarilise antimicrobialactivityandmodulatoryeffectofessentialoilfromtheleafofrhaphiodonechinusneesmartschaueronsomeantimicrobialdrugs |