Cargando…

Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita

Natural products, known for their medicinal properties since antiquity, are continuously being studied for their biological properties. In the present study, we analyzed the composition of the volatile preparations of essential oils of the Greek plants Ocimum basilicum (sweet basil), Mentha spicata...

Descripción completa

Detalles Bibliográficos
Autores principales: Fitsiou, Eleni, Mitropoulou, Gregoria, Spyridopoulou, Katerina, Tiptiri-Kourpeti, Angeliki, Vamvakias, Manolis, Bardouki, Haido, Panayiotidis, Mihalis Ι., Galanis, Alex, Kourkoutas, Yiannis, Chlichlia, Katerina, Pappa, Aglaia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274325/
https://www.ncbi.nlm.nih.gov/pubmed/27537869
http://dx.doi.org/10.3390/molecules21081069
_version_ 1783377592281202688
author Fitsiou, Eleni
Mitropoulou, Gregoria
Spyridopoulou, Katerina
Tiptiri-Kourpeti, Angeliki
Vamvakias, Manolis
Bardouki, Haido
Panayiotidis, Mihalis Ι.
Galanis, Alex
Kourkoutas, Yiannis
Chlichlia, Katerina
Pappa, Aglaia
author_facet Fitsiou, Eleni
Mitropoulou, Gregoria
Spyridopoulou, Katerina
Tiptiri-Kourpeti, Angeliki
Vamvakias, Manolis
Bardouki, Haido
Panayiotidis, Mihalis Ι.
Galanis, Alex
Kourkoutas, Yiannis
Chlichlia, Katerina
Pappa, Aglaia
author_sort Fitsiou, Eleni
collection PubMed
description Natural products, known for their medicinal properties since antiquity, are continuously being studied for their biological properties. In the present study, we analyzed the composition of the volatile preparations of essential oils of the Greek plants Ocimum basilicum (sweet basil), Mentha spicata (spearmint), Pimpinella anisum (anise) and Fortunella margarita (kumquat). GC/MS analyses revealed that the major components in the essential oil fractions, were carvone (85.4%) in spearmint, methyl chavicol (74.9%) in sweet basil, trans-anethole (88.1%) in anise, and limonene (93.8%) in kumquat. We further explored their biological potential by studying their antimicrobial, antioxidant and antiproliferative activities. Only the essential oils from spearmint and sweet basil demonstrated cytotoxicity against common foodborne bacteria, while all preparations were active against the fungi Saccharomyces cerevisiae and Aspergillus niger. Antioxidant evaluation by DPPH and ABTS radical scavenging activity assays revealed a variable degree of antioxidant potency. Finally, their antiproliferative potential was tested against a panel of human cancer cell lines and evaluated by using the sulforhodamine B (SRB) assay. All essential oil preparations exhibited a variable degree of antiproliferative activity, depending on the cancer model used, with the most potent one being sweet basil against an in vitro model of human colon carcinoma.
format Online
Article
Text
id pubmed-6274325
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62743252018-12-28 Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita Fitsiou, Eleni Mitropoulou, Gregoria Spyridopoulou, Katerina Tiptiri-Kourpeti, Angeliki Vamvakias, Manolis Bardouki, Haido Panayiotidis, Mihalis Ι. Galanis, Alex Kourkoutas, Yiannis Chlichlia, Katerina Pappa, Aglaia Molecules Article Natural products, known for their medicinal properties since antiquity, are continuously being studied for their biological properties. In the present study, we analyzed the composition of the volatile preparations of essential oils of the Greek plants Ocimum basilicum (sweet basil), Mentha spicata (spearmint), Pimpinella anisum (anise) and Fortunella margarita (kumquat). GC/MS analyses revealed that the major components in the essential oil fractions, were carvone (85.4%) in spearmint, methyl chavicol (74.9%) in sweet basil, trans-anethole (88.1%) in anise, and limonene (93.8%) in kumquat. We further explored their biological potential by studying their antimicrobial, antioxidant and antiproliferative activities. Only the essential oils from spearmint and sweet basil demonstrated cytotoxicity against common foodborne bacteria, while all preparations were active against the fungi Saccharomyces cerevisiae and Aspergillus niger. Antioxidant evaluation by DPPH and ABTS radical scavenging activity assays revealed a variable degree of antioxidant potency. Finally, their antiproliferative potential was tested against a panel of human cancer cell lines and evaluated by using the sulforhodamine B (SRB) assay. All essential oil preparations exhibited a variable degree of antiproliferative activity, depending on the cancer model used, with the most potent one being sweet basil against an in vitro model of human colon carcinoma. MDPI 2016-08-16 /pmc/articles/PMC6274325/ /pubmed/27537869 http://dx.doi.org/10.3390/molecules21081069 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fitsiou, Eleni
Mitropoulou, Gregoria
Spyridopoulou, Katerina
Tiptiri-Kourpeti, Angeliki
Vamvakias, Manolis
Bardouki, Haido
Panayiotidis, Mihalis Ι.
Galanis, Alex
Kourkoutas, Yiannis
Chlichlia, Katerina
Pappa, Aglaia
Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita
title Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita
title_full Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita
title_fullStr Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita
title_full_unstemmed Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita
title_short Phytochemical Profile and Evaluation of the Biological Activities of Essential Oils Derived from the Greek Aromatic Plant Species Ocimum basilicum, Mentha spicata, Pimpinella anisum and Fortunella margarita
title_sort phytochemical profile and evaluation of the biological activities of essential oils derived from the greek aromatic plant species ocimum basilicum, mentha spicata, pimpinella anisum and fortunella margarita
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274325/
https://www.ncbi.nlm.nih.gov/pubmed/27537869
http://dx.doi.org/10.3390/molecules21081069
work_keys_str_mv AT fitsioueleni phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT mitropoulougregoria phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT spyridopouloukaterina phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT tiptirikourpetiangeliki phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT vamvakiasmanolis phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT bardoukihaido phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT panayiotidismihalisi phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT galanisalex phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT kourkoutasyiannis phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT chlichliakaterina phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita
AT pappaaglaia phytochemicalprofileandevaluationofthebiologicalactivitiesofessentialoilsderivedfromthegreekaromaticplantspeciesocimumbasilicummenthaspicatapimpinellaanisumandfortunellamargarita