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Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings
In this work we try to address the large interest existing nowadays in the better understanding of the interaction between microbial biofilms and metallic implants. Our aimed was to identify a new preventive strategy to control drug release, biofilm formation and contamination of medical devices wit...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274373/ https://www.ncbi.nlm.nih.gov/pubmed/27294895 http://dx.doi.org/10.3390/molecules21060740 |
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author | Floroian, Laura Ristoscu, Carmen Mihailescu, Natalia Negut, Irina Badea, Mihaela Ursutiu, Doru Chifiriuc, Mariana Carmen Urzica, Iuliana Dyia, Hussien Mohammed Bleotu, Coralia Mihailescu, Ion N. |
author_facet | Floroian, Laura Ristoscu, Carmen Mihailescu, Natalia Negut, Irina Badea, Mihaela Ursutiu, Doru Chifiriuc, Mariana Carmen Urzica, Iuliana Dyia, Hussien Mohammed Bleotu, Coralia Mihailescu, Ion N. |
author_sort | Floroian, Laura |
collection | PubMed |
description | In this work we try to address the large interest existing nowadays in the better understanding of the interaction between microbial biofilms and metallic implants. Our aimed was to identify a new preventive strategy to control drug release, biofilm formation and contamination of medical devices with microbes. The transfer and printing of novel bioactive glass-polymer-antibiotic composites by Matrix-Assisted Pulsed Laser Evaporation into uniform thin films onto 316 L stainless steel substrates of the type used in implants are reported. The targets were prepared by freezing in liquid nitrogen mixtures containing polymer and antibiotic reinforced with bioglass powder. The cryogenic targets were submitted to multipulse evaporation by irradiation with an UV KrF* (λ = 248 nm, τ(FWHM) ≤ 25 ns) excimer laser source. The prepared structures were analyzed by infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and profilometry, before and after immersion in physiological fluids. The bioactivity and the release of the antibiotic have been evaluated. We showed that the incorporated antibiotic underwent a gradually dissolution in physiological fluids thus supporting a high local treatment efficiency. Electrochemical measurements including linear sweep voltammetry and impedance spectroscopy studies were carried out to investigate the corrosion resistance of the coatings in physiological environments. The in vitro biocompatibility assay using the MG63 mammalian cell line revealed that the obtained nanostructured composite films are non-cytotoxic. The antimicrobial effect of the coatings was tested against Staphylococcus aureus and Escherichia coli strains, usually present in implant-associated infections. An anti-biofilm activity was evidenced, stronger against E. coli than the S. aureus strain. The results proved that the applied method allows for the fabrication of implantable biomaterials which shield metal ion release and possess increased biocompatibility and resistance to microbial colonization and biofilm growth. |
format | Online Article Text |
id | pubmed-6274373 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62743732018-12-28 Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings Floroian, Laura Ristoscu, Carmen Mihailescu, Natalia Negut, Irina Badea, Mihaela Ursutiu, Doru Chifiriuc, Mariana Carmen Urzica, Iuliana Dyia, Hussien Mohammed Bleotu, Coralia Mihailescu, Ion N. Molecules Article In this work we try to address the large interest existing nowadays in the better understanding of the interaction between microbial biofilms and metallic implants. Our aimed was to identify a new preventive strategy to control drug release, biofilm formation and contamination of medical devices with microbes. The transfer and printing of novel bioactive glass-polymer-antibiotic composites by Matrix-Assisted Pulsed Laser Evaporation into uniform thin films onto 316 L stainless steel substrates of the type used in implants are reported. The targets were prepared by freezing in liquid nitrogen mixtures containing polymer and antibiotic reinforced with bioglass powder. The cryogenic targets were submitted to multipulse evaporation by irradiation with an UV KrF* (λ = 248 nm, τ(FWHM) ≤ 25 ns) excimer laser source. The prepared structures were analyzed by infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and profilometry, before and after immersion in physiological fluids. The bioactivity and the release of the antibiotic have been evaluated. We showed that the incorporated antibiotic underwent a gradually dissolution in physiological fluids thus supporting a high local treatment efficiency. Electrochemical measurements including linear sweep voltammetry and impedance spectroscopy studies were carried out to investigate the corrosion resistance of the coatings in physiological environments. The in vitro biocompatibility assay using the MG63 mammalian cell line revealed that the obtained nanostructured composite films are non-cytotoxic. The antimicrobial effect of the coatings was tested against Staphylococcus aureus and Escherichia coli strains, usually present in implant-associated infections. An anti-biofilm activity was evidenced, stronger against E. coli than the S. aureus strain. The results proved that the applied method allows for the fabrication of implantable biomaterials which shield metal ion release and possess increased biocompatibility and resistance to microbial colonization and biofilm growth. MDPI 2016-06-09 /pmc/articles/PMC6274373/ /pubmed/27294895 http://dx.doi.org/10.3390/molecules21060740 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Floroian, Laura Ristoscu, Carmen Mihailescu, Natalia Negut, Irina Badea, Mihaela Ursutiu, Doru Chifiriuc, Mariana Carmen Urzica, Iuliana Dyia, Hussien Mohammed Bleotu, Coralia Mihailescu, Ion N. Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings |
title | Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings |
title_full | Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings |
title_fullStr | Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings |
title_full_unstemmed | Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings |
title_short | Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings |
title_sort | functionalized antimicrobial composite thin films printing for stainless steel implant coatings |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274373/ https://www.ncbi.nlm.nih.gov/pubmed/27294895 http://dx.doi.org/10.3390/molecules21060740 |
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