Cargando…

Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings

In this work we try to address the large interest existing nowadays in the better understanding of the interaction between microbial biofilms and metallic implants. Our aimed was to identify a new preventive strategy to control drug release, biofilm formation and contamination of medical devices wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Floroian, Laura, Ristoscu, Carmen, Mihailescu, Natalia, Negut, Irina, Badea, Mihaela, Ursutiu, Doru, Chifiriuc, Mariana Carmen, Urzica, Iuliana, Dyia, Hussien Mohammed, Bleotu, Coralia, Mihailescu, Ion N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274373/
https://www.ncbi.nlm.nih.gov/pubmed/27294895
http://dx.doi.org/10.3390/molecules21060740
_version_ 1783377602993455104
author Floroian, Laura
Ristoscu, Carmen
Mihailescu, Natalia
Negut, Irina
Badea, Mihaela
Ursutiu, Doru
Chifiriuc, Mariana Carmen
Urzica, Iuliana
Dyia, Hussien Mohammed
Bleotu, Coralia
Mihailescu, Ion N.
author_facet Floroian, Laura
Ristoscu, Carmen
Mihailescu, Natalia
Negut, Irina
Badea, Mihaela
Ursutiu, Doru
Chifiriuc, Mariana Carmen
Urzica, Iuliana
Dyia, Hussien Mohammed
Bleotu, Coralia
Mihailescu, Ion N.
author_sort Floroian, Laura
collection PubMed
description In this work we try to address the large interest existing nowadays in the better understanding of the interaction between microbial biofilms and metallic implants. Our aimed was to identify a new preventive strategy to control drug release, biofilm formation and contamination of medical devices with microbes. The transfer and printing of novel bioactive glass-polymer-antibiotic composites by Matrix-Assisted Pulsed Laser Evaporation into uniform thin films onto 316 L stainless steel substrates of the type used in implants are reported. The targets were prepared by freezing in liquid nitrogen mixtures containing polymer and antibiotic reinforced with bioglass powder. The cryogenic targets were submitted to multipulse evaporation by irradiation with an UV KrF* (λ = 248 nm, τ(FWHM) ≤ 25 ns) excimer laser source. The prepared structures were analyzed by infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and profilometry, before and after immersion in physiological fluids. The bioactivity and the release of the antibiotic have been evaluated. We showed that the incorporated antibiotic underwent a gradually dissolution in physiological fluids thus supporting a high local treatment efficiency. Electrochemical measurements including linear sweep voltammetry and impedance spectroscopy studies were carried out to investigate the corrosion resistance of the coatings in physiological environments. The in vitro biocompatibility assay using the MG63 mammalian cell line revealed that the obtained nanostructured composite films are non-cytotoxic. The antimicrobial effect of the coatings was tested against Staphylococcus aureus and Escherichia coli strains, usually present in implant-associated infections. An anti-biofilm activity was evidenced, stronger against E. coli than the S. aureus strain. The results proved that the applied method allows for the fabrication of implantable biomaterials which shield metal ion release and possess increased biocompatibility and resistance to microbial colonization and biofilm growth.
format Online
Article
Text
id pubmed-6274373
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-62743732018-12-28 Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings Floroian, Laura Ristoscu, Carmen Mihailescu, Natalia Negut, Irina Badea, Mihaela Ursutiu, Doru Chifiriuc, Mariana Carmen Urzica, Iuliana Dyia, Hussien Mohammed Bleotu, Coralia Mihailescu, Ion N. Molecules Article In this work we try to address the large interest existing nowadays in the better understanding of the interaction between microbial biofilms and metallic implants. Our aimed was to identify a new preventive strategy to control drug release, biofilm formation and contamination of medical devices with microbes. The transfer and printing of novel bioactive glass-polymer-antibiotic composites by Matrix-Assisted Pulsed Laser Evaporation into uniform thin films onto 316 L stainless steel substrates of the type used in implants are reported. The targets were prepared by freezing in liquid nitrogen mixtures containing polymer and antibiotic reinforced with bioglass powder. The cryogenic targets were submitted to multipulse evaporation by irradiation with an UV KrF* (λ = 248 nm, τ(FWHM) ≤ 25 ns) excimer laser source. The prepared structures were analyzed by infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray spectroscopy and profilometry, before and after immersion in physiological fluids. The bioactivity and the release of the antibiotic have been evaluated. We showed that the incorporated antibiotic underwent a gradually dissolution in physiological fluids thus supporting a high local treatment efficiency. Electrochemical measurements including linear sweep voltammetry and impedance spectroscopy studies were carried out to investigate the corrosion resistance of the coatings in physiological environments. The in vitro biocompatibility assay using the MG63 mammalian cell line revealed that the obtained nanostructured composite films are non-cytotoxic. The antimicrobial effect of the coatings was tested against Staphylococcus aureus and Escherichia coli strains, usually present in implant-associated infections. An anti-biofilm activity was evidenced, stronger against E. coli than the S. aureus strain. The results proved that the applied method allows for the fabrication of implantable biomaterials which shield metal ion release and possess increased biocompatibility and resistance to microbial colonization and biofilm growth. MDPI 2016-06-09 /pmc/articles/PMC6274373/ /pubmed/27294895 http://dx.doi.org/10.3390/molecules21060740 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Floroian, Laura
Ristoscu, Carmen
Mihailescu, Natalia
Negut, Irina
Badea, Mihaela
Ursutiu, Doru
Chifiriuc, Mariana Carmen
Urzica, Iuliana
Dyia, Hussien Mohammed
Bleotu, Coralia
Mihailescu, Ion N.
Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings
title Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings
title_full Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings
title_fullStr Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings
title_full_unstemmed Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings
title_short Functionalized Antimicrobial Composite Thin Films Printing for Stainless Steel Implant Coatings
title_sort functionalized antimicrobial composite thin films printing for stainless steel implant coatings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274373/
https://www.ncbi.nlm.nih.gov/pubmed/27294895
http://dx.doi.org/10.3390/molecules21060740
work_keys_str_mv AT floroianlaura functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT ristoscucarmen functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT mihailescunatalia functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT negutirina functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT badeamihaela functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT ursutiudoru functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT chifiriucmarianacarmen functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT urzicaiuliana functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT dyiahussienmohammed functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT bleotucoralia functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings
AT mihailescuionn functionalizedantimicrobialcompositethinfilmsprintingforstainlesssteelimplantcoatings