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DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole

The DNA binding property of 5,10,15-Tris(4-carboxyphenyl) corrole (TCPC) was studied by UV-Visible, fluorescence and circular dichroism (CD) spectroscopic methods. TCPC can bind to ct-DNA via an outside binding mode with the binding constant of K(b) = 1.05 × 10(5) M(−1). TCPC also displayed good pho...

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Detalles Bibliográficos
Autores principales: Na, Ning, Zhao, Da-Qiang, Li, Heng, Jiang, Nan, Wen, Jin-Yan, Liu, Hai-Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274405/
https://www.ncbi.nlm.nih.gov/pubmed/26729089
http://dx.doi.org/10.3390/molecules21010054
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author Na, Ning
Zhao, Da-Qiang
Li, Heng
Jiang, Nan
Wen, Jin-Yan
Liu, Hai-Yang
author_facet Na, Ning
Zhao, Da-Qiang
Li, Heng
Jiang, Nan
Wen, Jin-Yan
Liu, Hai-Yang
author_sort Na, Ning
collection PubMed
description The DNA binding property of 5,10,15-Tris(4-carboxyphenyl) corrole (TCPC) was studied by UV-Visible, fluorescence and circular dichroism (CD) spectroscopic methods. TCPC can bind to ct-DNA via an outside binding mode with the binding constant of K(b) = 1.05 × 10(5) M(−1). TCPC also displayed good photonuclease activity, which involves singlet oxygen species ((1)O(2)). The binding constant between TCPC and human serum albumin (HSA) is K(A) = 2.24 × 10(5) M(−1) with a simulated binding distance of 2.06 nm. The fluorescence quenching of HSA by TCPC followed a static quenching process. Site marker competitive displacement experiments indicated that warfarin site I is the main binding site. The secondary structure of HSA was changed upon interaction with TCPC, which was confirmed by UV-Visible and CD spectroscopy.
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spelling pubmed-62744052018-12-28 DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole Na, Ning Zhao, Da-Qiang Li, Heng Jiang, Nan Wen, Jin-Yan Liu, Hai-Yang Molecules Article The DNA binding property of 5,10,15-Tris(4-carboxyphenyl) corrole (TCPC) was studied by UV-Visible, fluorescence and circular dichroism (CD) spectroscopic methods. TCPC can bind to ct-DNA via an outside binding mode with the binding constant of K(b) = 1.05 × 10(5) M(−1). TCPC also displayed good photonuclease activity, which involves singlet oxygen species ((1)O(2)). The binding constant between TCPC and human serum albumin (HSA) is K(A) = 2.24 × 10(5) M(−1) with a simulated binding distance of 2.06 nm. The fluorescence quenching of HSA by TCPC followed a static quenching process. Site marker competitive displacement experiments indicated that warfarin site I is the main binding site. The secondary structure of HSA was changed upon interaction with TCPC, which was confirmed by UV-Visible and CD spectroscopy. MDPI 2015-12-31 /pmc/articles/PMC6274405/ /pubmed/26729089 http://dx.doi.org/10.3390/molecules21010054 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Na, Ning
Zhao, Da-Qiang
Li, Heng
Jiang, Nan
Wen, Jin-Yan
Liu, Hai-Yang
DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole
title DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole
title_full DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole
title_fullStr DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole
title_full_unstemmed DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole
title_short DNA Binding, Photonuclease Activity and Human Serum Albumin Interaction of a Water-Soluble Freebase Carboxyl Corrole
title_sort dna binding, photonuclease activity and human serum albumin interaction of a water-soluble freebase carboxyl corrole
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274405/
https://www.ncbi.nlm.nih.gov/pubmed/26729089
http://dx.doi.org/10.3390/molecules21010054
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