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Elucidation of Differential Accumulation of 1-Phenylethanol in Flowers and Leaves of Tea (Camellia sinensis) Plants

1-Phenylethanol (1PE) is a major aromatic volatile in tea (Camellia sinensis) flowers, whereas it occurs in a much smaller amounts in leaves. Enzymes involved in the formation of 1PE in plants and the reason why 1PE differentially accumulates in plants is unknown. In the present study, enzymes in th...

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Autores principales: Dong, Fang, Zhou, Ying, Zeng, Lanting, Peng, Qiyuan, Chen, Yiyong, Zhang, Ling, Su, Xinguo, Watanabe, Naoharu, Yang, Ziyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274408/
https://www.ncbi.nlm.nih.gov/pubmed/27563859
http://dx.doi.org/10.3390/molecules21091106
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author Dong, Fang
Zhou, Ying
Zeng, Lanting
Peng, Qiyuan
Chen, Yiyong
Zhang, Ling
Su, Xinguo
Watanabe, Naoharu
Yang, Ziyin
author_facet Dong, Fang
Zhou, Ying
Zeng, Lanting
Peng, Qiyuan
Chen, Yiyong
Zhang, Ling
Su, Xinguo
Watanabe, Naoharu
Yang, Ziyin
author_sort Dong, Fang
collection PubMed
description 1-Phenylethanol (1PE) is a major aromatic volatile in tea (Camellia sinensis) flowers, whereas it occurs in a much smaller amounts in leaves. Enzymes involved in the formation of 1PE in plants and the reason why 1PE differentially accumulates in plants is unknown. In the present study, enzymes in the last step leading from acetophenone to 1PE were isolated from tea flowers by traditional biochemical chromatography. The two types of partially purified enzymes were proposed to be responsible for formations of (R)-1PE and (S)-1PE, respectively. Tea leaves also contained such enzymes having equivalent activities with flowers. Stable isotope labeling experiments indicated that weak transformation from l-phenylalanine to acetophenone in leaves mainly resulted in little occurrence of 1PE in leaves. This study provided an example that differential distribution of some metabolites in plant tissues was not only determined by enzyme(s) in the last step of metabolite formation, but also can be due to substrate availability.
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spelling pubmed-62744082018-12-28 Elucidation of Differential Accumulation of 1-Phenylethanol in Flowers and Leaves of Tea (Camellia sinensis) Plants Dong, Fang Zhou, Ying Zeng, Lanting Peng, Qiyuan Chen, Yiyong Zhang, Ling Su, Xinguo Watanabe, Naoharu Yang, Ziyin Molecules Article 1-Phenylethanol (1PE) is a major aromatic volatile in tea (Camellia sinensis) flowers, whereas it occurs in a much smaller amounts in leaves. Enzymes involved in the formation of 1PE in plants and the reason why 1PE differentially accumulates in plants is unknown. In the present study, enzymes in the last step leading from acetophenone to 1PE were isolated from tea flowers by traditional biochemical chromatography. The two types of partially purified enzymes were proposed to be responsible for formations of (R)-1PE and (S)-1PE, respectively. Tea leaves also contained such enzymes having equivalent activities with flowers. Stable isotope labeling experiments indicated that weak transformation from l-phenylalanine to acetophenone in leaves mainly resulted in little occurrence of 1PE in leaves. This study provided an example that differential distribution of some metabolites in plant tissues was not only determined by enzyme(s) in the last step of metabolite formation, but also can be due to substrate availability. MDPI 2016-08-23 /pmc/articles/PMC6274408/ /pubmed/27563859 http://dx.doi.org/10.3390/molecules21091106 Text en © 2016 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dong, Fang
Zhou, Ying
Zeng, Lanting
Peng, Qiyuan
Chen, Yiyong
Zhang, Ling
Su, Xinguo
Watanabe, Naoharu
Yang, Ziyin
Elucidation of Differential Accumulation of 1-Phenylethanol in Flowers and Leaves of Tea (Camellia sinensis) Plants
title Elucidation of Differential Accumulation of 1-Phenylethanol in Flowers and Leaves of Tea (Camellia sinensis) Plants
title_full Elucidation of Differential Accumulation of 1-Phenylethanol in Flowers and Leaves of Tea (Camellia sinensis) Plants
title_fullStr Elucidation of Differential Accumulation of 1-Phenylethanol in Flowers and Leaves of Tea (Camellia sinensis) Plants
title_full_unstemmed Elucidation of Differential Accumulation of 1-Phenylethanol in Flowers and Leaves of Tea (Camellia sinensis) Plants
title_short Elucidation of Differential Accumulation of 1-Phenylethanol in Flowers and Leaves of Tea (Camellia sinensis) Plants
title_sort elucidation of differential accumulation of 1-phenylethanol in flowers and leaves of tea (camellia sinensis) plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274408/
https://www.ncbi.nlm.nih.gov/pubmed/27563859
http://dx.doi.org/10.3390/molecules21091106
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