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Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis

The fat body plays key roles in energy storage and utilization as well as biosynthetic and metabolic activities in insects. During metamorphosis from larva to pupa, the fat body undergoes dramatic changes in morphology and metabolic processes. However, the genetic basis underlying these changes has...

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Autores principales: Peng, Jian, Li, Zheng, Yang, Yan, Wang, Peng, Zhou, Xuan, Zhao, Tujing, Guo, Mengpei, Meng, Meng, Zhang, Tianlei, Qian, Wenliang, Xia, Qingyou, Cheng, Daojun, Zhao, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274779/
https://www.ncbi.nlm.nih.gov/pubmed/30423910
http://dx.doi.org/10.3390/ijms19113525
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author Peng, Jian
Li, Zheng
Yang, Yan
Wang, Peng
Zhou, Xuan
Zhao, Tujing
Guo, Mengpei
Meng, Meng
Zhang, Tianlei
Qian, Wenliang
Xia, Qingyou
Cheng, Daojun
Zhao, Ping
author_facet Peng, Jian
Li, Zheng
Yang, Yan
Wang, Peng
Zhou, Xuan
Zhao, Tujing
Guo, Mengpei
Meng, Meng
Zhang, Tianlei
Qian, Wenliang
Xia, Qingyou
Cheng, Daojun
Zhao, Ping
author_sort Peng, Jian
collection PubMed
description The fat body plays key roles in energy storage and utilization as well as biosynthetic and metabolic activities in insects. During metamorphosis from larva to pupa, the fat body undergoes dramatic changes in morphology and metabolic processes. However, the genetic basis underlying these changes has not been completely understood. In this study, the authors performed a time-course transcriptome analysis of the fat body during silkworm metamorphosis using RNA-sequencing. A total of 5217 differentially expressed genes (DEGs) were identified in the fat body at different developmental time points. DEGs involved in lipid synthesis and degradation were highly expressed at the third day of the last larval instar and during the prepupal-pupal transition, respectively. DEGs involved in the ecdysone signaling and bone morphogenetic protein (BMP) signaling pathways that modulate organ development exhibited a high expression level during the fat body remodeling process from prepupa to pupa. Intriguingly, the RNA interference-mediated knockdown of either decapentaplegic (Dpp) or protein 60A (Gbb), two DEGs involved in the BMP signaling pathway, inhibited fat body dissociation but promoted lipid mobilization, suggesting that the BMP signaling pathway not only is required for fat body remodeling, but also moderately inhibits lipid mobilization to ensure an appropriate lipid supply during the pupal-adult transition. In conclusion, the comparative transcriptome analysis provides novel insight into morphologic and metabolic changes in the fat body during silkworm metamorphosis.
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spelling pubmed-62747792018-12-15 Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis Peng, Jian Li, Zheng Yang, Yan Wang, Peng Zhou, Xuan Zhao, Tujing Guo, Mengpei Meng, Meng Zhang, Tianlei Qian, Wenliang Xia, Qingyou Cheng, Daojun Zhao, Ping Int J Mol Sci Article The fat body plays key roles in energy storage and utilization as well as biosynthetic and metabolic activities in insects. During metamorphosis from larva to pupa, the fat body undergoes dramatic changes in morphology and metabolic processes. However, the genetic basis underlying these changes has not been completely understood. In this study, the authors performed a time-course transcriptome analysis of the fat body during silkworm metamorphosis using RNA-sequencing. A total of 5217 differentially expressed genes (DEGs) were identified in the fat body at different developmental time points. DEGs involved in lipid synthesis and degradation were highly expressed at the third day of the last larval instar and during the prepupal-pupal transition, respectively. DEGs involved in the ecdysone signaling and bone morphogenetic protein (BMP) signaling pathways that modulate organ development exhibited a high expression level during the fat body remodeling process from prepupa to pupa. Intriguingly, the RNA interference-mediated knockdown of either decapentaplegic (Dpp) or protein 60A (Gbb), two DEGs involved in the BMP signaling pathway, inhibited fat body dissociation but promoted lipid mobilization, suggesting that the BMP signaling pathway not only is required for fat body remodeling, but also moderately inhibits lipid mobilization to ensure an appropriate lipid supply during the pupal-adult transition. In conclusion, the comparative transcriptome analysis provides novel insight into morphologic and metabolic changes in the fat body during silkworm metamorphosis. MDPI 2018-11-09 /pmc/articles/PMC6274779/ /pubmed/30423910 http://dx.doi.org/10.3390/ijms19113525 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peng, Jian
Li, Zheng
Yang, Yan
Wang, Peng
Zhou, Xuan
Zhao, Tujing
Guo, Mengpei
Meng, Meng
Zhang, Tianlei
Qian, Wenliang
Xia, Qingyou
Cheng, Daojun
Zhao, Ping
Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis
title Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis
title_full Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis
title_fullStr Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis
title_full_unstemmed Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis
title_short Comparative Transcriptome Analysis Provides Novel Insight into Morphologic and Metabolic Changes in the Fat Body during Silkworm Metamorphosis
title_sort comparative transcriptome analysis provides novel insight into morphologic and metabolic changes in the fat body during silkworm metamorphosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274779/
https://www.ncbi.nlm.nih.gov/pubmed/30423910
http://dx.doi.org/10.3390/ijms19113525
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