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Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering
The quaternary transition between the relaxed (R) and tense (T) states of heme-binding proteins is a textbook example for the allosteric structural transition. Homodimeric hemoglobin (HbI) from Scapharca inaequivalvis is a useful model system for investigating the allosteric behavior because of the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274816/ https://www.ncbi.nlm.nih.gov/pubmed/30453670 http://dx.doi.org/10.3390/ijms19113633 |
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author | Yang, Cheolhee Choi, Minseo Kim, Jong Goo Kim, Hanui Muniyappan, Srinivasan Nozawa, Shunsuke Adachi, Shin-ichi Henning, Robert Kosheleva, Irina Ihee, Hyotcherl |
author_facet | Yang, Cheolhee Choi, Minseo Kim, Jong Goo Kim, Hanui Muniyappan, Srinivasan Nozawa, Shunsuke Adachi, Shin-ichi Henning, Robert Kosheleva, Irina Ihee, Hyotcherl |
author_sort | Yang, Cheolhee |
collection | PubMed |
description | The quaternary transition between the relaxed (R) and tense (T) states of heme-binding proteins is a textbook example for the allosteric structural transition. Homodimeric hemoglobin (HbI) from Scapharca inaequivalvis is a useful model system for investigating the allosteric behavior because of the relatively simple quaternary structure. To understand the cooperative transition of HbI, wild-type and mutants of HbI have been studied by using time-resolved X-ray solution scattering (TRXSS), which is sensitive to the conformational changes. Herein, we review the structural dynamics of HbI investigated by TRXSS and compare the results of TRXSS with those of other techniques. |
format | Online Article Text |
id | pubmed-6274816 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-62748162018-12-15 Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering Yang, Cheolhee Choi, Minseo Kim, Jong Goo Kim, Hanui Muniyappan, Srinivasan Nozawa, Shunsuke Adachi, Shin-ichi Henning, Robert Kosheleva, Irina Ihee, Hyotcherl Int J Mol Sci Review The quaternary transition between the relaxed (R) and tense (T) states of heme-binding proteins is a textbook example for the allosteric structural transition. Homodimeric hemoglobin (HbI) from Scapharca inaequivalvis is a useful model system for investigating the allosteric behavior because of the relatively simple quaternary structure. To understand the cooperative transition of HbI, wild-type and mutants of HbI have been studied by using time-resolved X-ray solution scattering (TRXSS), which is sensitive to the conformational changes. Herein, we review the structural dynamics of HbI investigated by TRXSS and compare the results of TRXSS with those of other techniques. MDPI 2018-11-18 /pmc/articles/PMC6274816/ /pubmed/30453670 http://dx.doi.org/10.3390/ijms19113633 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Yang, Cheolhee Choi, Minseo Kim, Jong Goo Kim, Hanui Muniyappan, Srinivasan Nozawa, Shunsuke Adachi, Shin-ichi Henning, Robert Kosheleva, Irina Ihee, Hyotcherl Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering |
title | Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering |
title_full | Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering |
title_fullStr | Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering |
title_full_unstemmed | Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering |
title_short | Protein Structural Dynamics of Wild-Type and Mutant Homodimeric Hemoglobin Studied by Time-Resolved X-Ray Solution Scattering |
title_sort | protein structural dynamics of wild-type and mutant homodimeric hemoglobin studied by time-resolved x-ray solution scattering |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6274816/ https://www.ncbi.nlm.nih.gov/pubmed/30453670 http://dx.doi.org/10.3390/ijms19113633 |
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